Anatomy and Cell Biology

Korean Association of Anatomists (대한해부학회)
권호 표지

The Effect of BF-7 on the Ischemia-induced Learning and Memory Deficits

BF-7의 뇌보호작용 및 학습과 기억력 개선 효과

Lee, Jun-Yeong;Lee, Sang-Hyeong;Seong, Jeong-Jun;Kim, Ui-Tae;Jo, Hye-Jin;Kim, Gyeong-Hwan;Gang, Yong-Gu;Kim, Seong-Su;Gwon, O-Sang;Lee, Won-Bok
이준영;이상형;성정준;김의태;조혜진;김경환;강용구;김성수;권오상;이원복

  • Published : 20050400
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Abstract

Abnormal blood supply to brain, such as ischemia induce neuronal damages, possibly leading to dementia. Such damages should be attenuated by neuroprotective materials which make neurons tolerable against limited blood supply or reinforce of blood. For the animal study, transient middle cerebral artery occlusion was operated with SD rat. To check whether BF-7 attenuated the ischemic damage, BF-7 (10 mg/kg) was oral treated for 7days once a day. To evaluate the learning and memory of the rat, 8-arm maze test was conducted. For clinical study, Rey Complex Figure Test (RCFT) was used with control group (32 person), 200 mg treated group (33 person) and 400mg treated group (34 person). Treatment of BF-7 greatly reduced the infarct size. Also the neuronal damages in the hippocampus were significantly diminished. Furthermore, The memory impairment by ischemia was recovered. Theclinical test showed that BF-7 greatly enhanced the brain function recognizing and memorizing complex two dimensional figures. Our results implicated that BF-7 plays a positive roles on protecting brain and enhancing brain function clinically.

허혈과 같이 뇌로 비정상적인 혈액 공급이 이루어지는 경우 신경세포의 손상을 가져오며 이러한 신경세포의 손상은 여러 신경세포 보호물질에 의해 저해될 수 있다. 본 연구에서는 가잠 추출물인 BF-7의 보호효과를 확인하고자 동물실험과 임상시험을 실시하였으며, 동물실험을 위하여 SD rat의 중간대뇌동맥의 일시적인결찰을 유도하고, 허혈성 손상에 대해 BF-7의 보호작용여부를 확인하고자 BF-7 (10 mg/kg)을 하루에 한번씩 7일간 경구 투여하였다. 또한 쥐의 기억과 학습능력을 시험하기 위하여 방사형 8방미로 시험을 시행하였다. 또한Rey Complex Figure Test (RCFT) 방법을 이용하여 대조군(32명), 200 mg 투여군(33명)과 400 mg 투여군(34명)을대상으로 임상시험을 실시하였다. 동물실험의 결과 BF-7을 경구 투여한 경우 infarct size가 현저하게 감소함을확인할 수 있었고, 해마의 신경세포 손상 역시 의미 있게 감소하였음을 확인하였다. 더욱이 허혈에 의한 기억력손상도 BF-7에 의해 회복될 수 있었다. 또한 임상시험의 결과 BF-7이 복잡한 2차원 그림을 기억하고 인지하는두뇌기능이 현저하게 활성화되었다. 따라서 본 연구의 결과는 BF-7이 두뇌를 보호하고, 두뇌기능을 활성화 시키는 데 효과적으로 작용하는 것을 보여주었고, BF-7의 기능과 역할에 대한 연구를 통해 신경 세포 손상을 효과적으로 치료할 수 있을 것으로 생각된다.

Keywords

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