Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Evaluation of Genotoxicity in Blood Cells of a Polychaetous Worm (Perinereis aibuhitensis), Using Comet Assay

Comet assay를 이용한 갯지렁이 (Perinereis aibuhitensis)의 혈구세포에 대한 유전독성 평가

  • Seo Jin Young (Department of Marine Environmental Engineering, Marine Industry Institute, Gyeongsang National University) ;
  • Sung Chan Gyoung (Department of Marine Environmental Engineering, Marine Industry Institute, Gyeongsang National University) ;
  • Choi Jin Woo (South Sea Institute, KORDI) ;
  • Lee Chang Hoon (South Sea Institute, KORDI) ;
  • Ryul Tae Kwon (South Sea Institute, KORDI) ;
  • Han Gi Myung (South Sea Institute, KORDI) ;
  • Kim Gi Beum (Department of Marine Environmental Engineering, Marine Industry Institute, Gyeongsang National University)
  • 서진영 (경상대학교 해양환경공학과 해양산업연구소) ;
  • 성찬경 (경상대학교 해양환경공학과 해양산업연구소) ;
  • 최진우 (한국해양연구원 남해연구소) ;
  • 이창훈 (한국해양연구원 남해연구소) ;
  • 류태권 (한국해양연구원 남해연구소) ;
  • 한기명 (한국해양연구원 남해연구소) ;
  • 김기범 (경상대학교 해양환경공학과 해양산업연구소)
  • Published : 2005.12.01
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Abstract

In order to know whether polychaetes could be used as an appropriate organism for the detection of genotoxicity, DNA strand breaks were evaluated in blood cells of a nereidae worm (Perinereis aibuhitensis) exposed to various aquatic chemical pollutants (e.g. Cd, Pb, Pyrene, Benaor[a]pyrene). Hydrogen peroxide increased DNA strand breaks up to the highest concentration (10 $\mu$M). Higher concentration than 0.1 $\mu$M showed a significantly more DNA damage than control. Cadmium and lead also showed higher DNA damage than control, over 1.0 and 1 $\mu$g/L, respectively. In case of pyrene, DNA damage was detected even at 0.001 $\mu$g/L. However, DNA damage decreased due to apoptosis at the highest concentration of pyrene and Pb. This study suggested that the polythaetous blood cells could be used effectively for screening genotoxic contaminants in the environment.

Keywords

References

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