Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Isolation and Quantitative Analysis of Alisol B 23-Acetate from the Rhizome of Alisma orientale

택사에서 Alisol B 23-Acetate의 분리 및 함량분석

  • 박종철 (순천대학교 한약자원학과 및 한의약연구소) ;
  • 허종문 (순천대학교 한약자원학과 및 한의약연구소) ;
  • 김세은 (순천대학교 한약자원학과 및 한의약연구소)
  • Published : 2005.02.01
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Abstract

Alismatis Rhizoma is an oriental medicine originated from the rhizome of Alisma orientale or Alisma plangtago-aquatica var. orientale (Alismataceae). As an standard compound of this plant, alisol B 23-acetate was isolated from the dichloromethane fraction of Alisma orientale and identified by the spectroscopic evidences. A Quantitative analysis of alisol B 23-acetate using HPLC method showed that the average content was 0.47$\pm$0.11% in 33 samples throughout the various regions of Korea.

택사 MeOH 추출물에서 얻은 dichlorornethane 분획물을 칼럼 크로마토그라피를 실시하여 alisol B 23-acetate를 순수히 분리하였다. Alisol B 23-acetate를 여러가지 용매와 추출 조건을 달리하여 HPLC를 실시하여 이들의 분리능을 검토한 결과 70% $CH_3$CN 혼합용매를 사용할 때 분리능이 가장 양호하였다. 국내 각지에서 수집한 택사 33종과 중국 현지에서 수집한 중국택사 5종에 대해 HPLC를 이용하여 함량분석을 하였다. 국내구입 검체중 지표성분 alisol B 23-acetate의 함량은 0.25%에서 0.67%까지의 범위를 보였으며 평균값 및 표준편차가 0.47$\pm$0.11%이었다. 실험에 사용한 검체중 재배산지에서 직접 구입한 순천시 해룡산 택사가 alisol B 23-acetate의 함량이 0.67%로서 가장 많았다. 중국에서 구입한 택사에는 alisol B 23-acetate가 포함되어 있지 않거나 0.27%에서 0.33%함유하였다. 이 검체들은 한국에서 구입한 택사와 종이 다르거나 또는 재배조건이 상이하여 이러한 결과가 나온 것으로 추정된다. 택사의 표준화를 위해서는 지표성 분 alisol B 23-acetate의 함량은 0.25% 이상으로 규정함이 타당하다고 사료된다.

Keywords

References

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