Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Semi-selective Medium for Monitoring Colletotrichum acutatum Causing Pepper Anthracnose in the Field

고추 탄저병균Colletotrichum acutatum 의 포장 밀도 조사를 위한 반선택 배지의 확립 및 활용

  • Kang, Beum-Kwan (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Min, Ji-Young (Department of Plant Pathology, The University of Nebraska) ;
  • Kim, Yun-Sik (Research Center of Bioactive Materials, Chonbuk National University) ;
  • Park, Sung-Woo (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Nguyen, Van-Bach (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University) ;
  • Kim, Heung-Tae (Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
  • 강범관 (충북대학교 농업생명환경대학 식물의학과) ;
  • 민지영 ;
  • 김윤식 (전북대학교 생명과학부) ;
  • 박성우 (충북대학교 농업생명환경대학 식물의학과) ;
  • ;
  • 김흥태 (충북대학교 농업생명환경대학 식물의학과)
  • Published : 2005.06.01
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Abstract

It was confirmed that anthracnose pathogen, Colletotrichum acutatum, could specifically grow on PDA amended with $100\mu\textrm{g}$ /ml of ampicillin and tetracycline, and 100 $100\mu\textrm{g}$/ml of mixture with carbendazim and diethofencarb. There was a positive correlation between the number of colony enumerated on semi-selective media and the disease severity on pepper fruits caused by C. acutatum. Using semi-selective media for C. acutatum, the number of pathogen on soil and plant debris infected by anthracnose pathogen was investigated. In plant debris, the colony number of C. acutatum was more than in soil. For the identification of colony appeared on semi-selective media, 10 isolates were selected randomly. They were identified as C. acutatum through PCR using C. acutatum-specific primer.

고추 탄저병균인(Colletotrichum acutatum)은 100$\mu\textrm{g}$ / $m\ell$ 의 ampicillin과 tetracycline과 같은 항생물질과 100$\mu\textrm{g}$ / $m\ell$의 carbendazim과 diethofencarb의 혼합체를 첨가한 PDA 배지에서 특이적으로 생장하였다. 탄저병의 발병 정도가 다른 고추 열매르 ㄹ갈아서 C.acutatum의 반선택 배지에 도말한 결과, 발병 정도와 반선택 배지에 나타나는 병원균의 균총 수는 정의 상관 관계를 나타냈다. 고추를 재배했던 포장에서 이병 잔재물과 토양을 채집하여 반선택 배지 상에서 병원균의 수를 조사한 결과, C.acutatum은 토양보다는 이병 잔재물에서 더 많은 수가 검출되었다. 반선택 배지에 나타난 병원균을 무작위로 10균주를 선발하여 동정하였다. 선발한 균주들의 DNA를 추출하여 C. acutatum 특이적인 printer를 사용한 PCR 을 수행한 결과, 모든 균주가 C. acutatum으로 확인되었다.

Keywords

References

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