The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지

Antimicrobial Effect of Plagiorhegama dubium Extracts on Food-borne Pathogen

식중독 유발세균의 증식에 미치는 황련 추출물의 효과

  • Bae Ji-Hyun (Department of Food Science and Nutrition, Keimyung University)
  • 배지현 (계명대학교 식품영양학과)
  • Published : 2005.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to investigate the antimicrobial activities of the Plagiorhegama dubium extracts against food-borne pathogens. The methanol extract was partitioned into petroleum ether, chloroform, ethyl acetate, and methanol portions. The antimicrobial activity of the P. dubium extracts was determined using a paper disc method against food-borne pathogens and food spoilage bacteria. The methanol extracts of P. dubium showed the highest antimicrobial activity against Staphylococcus aureus and Escherichia coli. The synergistic effect has been found in combined extracts of P. dubium and Hedyotis diffsa as compared to each extracts alone. Finally, the growth inhibition curve was determined using methanol extracts of P. dubium against S. aureus and E. coli. The methanol extract of P. dubium showed strong antimicrobial activity against S. aureus at the concentration of 4,000 ppm. The 4,000 ppm of ethyl acetate portion from P. dubium retarded the growth of S. aureus more than 36 hours and E. coli up to 60 hours. The methanol extracts of P. dubium has been shown the antimicrobial activity against S. aureus and E. coli.

본 연구에서는 식중독 유발 세균에 대한 항균 활성이 우수한 천연 항균성 물질을 검색하기 위해 예로부터 민간과 한방에서 널리 이용되어온 황련을 각종 유기용매로 추출하여 식중독 유발 세균에 대한 항균 활성을 조사해 보았다. 황련을 petroleum ether, chloroform, ethyl acetate, methanol를 이용하여 실온에서 각각 용매별로 계통 분획하고, 열수추출물을 얻은 후, 9종의 식중독 유발세균(Staphylococcus aureus, Bacillus cereus, Salmonella enteritidis, Shigella flexneri, Escherichia coli, Salmonella typhimurium, Shigella dysenteriae, Pseudomonas aruginosa, Shigella sonnei)에 대하여 항균효과를 조사하였다. 황련 추출물의 농도별 항균 활성 검색에서는 황련의 methanol 추출물이 가장 큰 항균효과를 보였으며 S. aureus와 E. coli가 가장 민감하게 반응하는 균주였다. 황련의 methanol 추출물과 백화사설초의 methanol 추출물을 혼합하여 항균력을 측정해 본 결과 두 추출물을 섞어 첨가했을 경우가 단독으로 사용했을 시 보다 상승효과를 나타내었다. 또한 황련의 methanol 추출물이 식중독 유발세균의 성장에 미치는 효과를 검정하기 위해 S. aureus 및 E. coli의 배양액에 황련의 methanol 추출물을 각각 4,000 ppm 농도로 첨가했을 시, E. coli이 생육이 60시간 이상까지 억제됨을 관찰할 수 있었고, S. aureus의 생육도 36시간까지 지연시킬 수 있었다. 이상의 결과 황련의 methanol 추출물은 S. aureus와 E. coli이 성장을 효과적으로 억제시킬 수 있음을 알 수 있었다.

Keywords

References

  1. Moon, BS. Food Sanitation, 2nd ed., pp. 75-116. Shinkwang Publishing Co. Seoul, Korea. 2003
  2. Kang, SK. Isolation and antimicrobial activity of antimicrobial substance obtained from leaf mustard (Brassica juncea). Kor. J. Food Sci. Technol. 24 :697-698. 1995
  3. Park, SK, Park, JR, Lee, SW, Seo, KI, Kim, SK and Shim, KH. Antimicrabial activity and heat stability of water-pretreated extract of leaf mustard dolsan(Brassica juncea). J. Kor. Soc. Food Nutr. 24:710-715. 1995
  4. Sin, KH, An, DK, Woo, ER, Park, HK, Lee, JS and Yun, WS. A Study on the extraction efficiency of berberine in Coptidis rhizoma. J. Kor. Analyical Sci. 10(2):83-91. 1997
  5. Kim, YJ, Park, JW, Lee, MJ, Kim, JK and Choi, DY. Nitrite scavenging activity of water-extract from Scutellaria baicalensis Georgi and Coptis chinensis Franch. J. Orintal Med. Pathol. 14(2):108-118. 2000
  6. Jang, GH, An BY, Oh SH, Choi DS and Kown YJ. Anticariogenic effects of Coptis chinensis Franch Extract. Kor. J. Food Sci. Technol. 32(6): 1396-1402 (2000)
  7. Cho, SH, Park, UP and Jung, SK. Changes in the keeping quality of strawberry and cucumber treated with Korean medical herb extracts. Kor. J. Gerontol. 7(2): 145-150. 2000
  8. James, GC and Sherman, J. Chemotheraperapeutic agent in microbiology, A laboratory manual chemical agents of control, 2nd ed., pp. 247-254. Prentice Hall, New Jersey, U.S.A. 1987
  9. Karapinar, M. Inhibitory effects of anethole and eugenol on the growth and toxin production of Aspergillus parasiticus. Int'l J. Food Microbiol. 10:193-200. 1990 https://doi.org/10.1016/0168-1605(90)90066-E
  10. Yoo, SJ, Lee, KB and Kwak, JH. Original articles;studies on the seasonal variation of berberine contents in Berberis koreana. Kor. J. Pharmacognosy. 17(2):123-128. 1986
  11. Park, JW, Kim, CH, Lee, MJ, Kim, JK and Choi, DY. Antioxidant and nitrite scavenging activity of water- extract from Phellodendron amurense Rupr. J. Orintal Med. Pathol. 13(1):112-118. 1999
  12. Lee, JA and Park IS. Growth retardation of Escherichia coli and Staphylococcus aureus by leek extract J. Food Sci. Nutr, 30(1):196-198. 2001
  13. Kim, SY, Sin, WS, Kim, MH and Lee, JS. Antimicrobial activity of garlic juice against Escherichia coli O157:H7 Kor. J. Food Sci. Technol. 35(4): 752-755. 2003
  14. Sim, KW, Kim, J, Cho, YS, Seo, KI and Ju, OS. Antimicrobial activities of Zanthoxylum schinifolium and Zanthoxylum piperitum leaves. Kor. J. Gerontol. 7(2): 195-200. 2000
  15. Heo, TR, Lee, YM Choi, SI and Chae, H. Research papers: Antimicrobial effects of herbal medicine extracts on Staphylococcus aureus and Escherichia coli O157:H7. Kor. J. Biotechnol. Bioengineering 17(6): 537-542. 2002
  16. Kim, DW, Nam, ES, Jung, CI and Joun HI. Studies on the biological function and antibacterial Effect of lactoperoxidase system in milk and antibacterial effect of lactoperoxidase system against Escherichia coli O157:H7. J. Kor. Food Hygiene Safety 13(1): 1-5. 1998
  17. Kim, CS, Jung, SK, Oh, YK and Kim, RY. Antimicrobial activity of green tea against putrefactive microorganism in steamed bread. J. Food Sci. Nutr. 32(3):413-417. 2003 https://doi.org/10.3746/jkfn.2003.32.3.413