Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Synergistic Effects of Low Dose Gamma Irradiation and Growth Regulators on Seed Germination, Growth and Photosynthesis in Rice (Oryza sativa L.)

벼의 종자 발아와 생육 및 광합성에 대한 저선량 감마선과 생장조절물질의 상승작용 효과

  • Baek Myung-Hwa (Radiation Application Research Team, Korea Atomic Energy Research Institute) ;
  • Chung Byung Yeoup (Radiation Application Research Team, Korea Atomic Energy Research Institute) ;
  • Kim Jin-Hong (Radiation Application Research Team, Korea Atomic Energy Research Institute) ;
  • Wi Seung Gon (Radiation Application Research Team, Korea Atomic Energy Research Institute) ;
  • Kim Jae-Sung (Radiation Application Research Team, Korea Atomic Energy Research Institute) ;
  • Lee In-Jung (Department of Agronomy, Kyungpook National University)
  • 백명화 (한국원자력연구소 방사선이용연구부) ;
  • 정병엽 (한국원자력연구소 방사선이용연구부) ;
  • 김진홍 (한국원자력연구소 방사선이용연구부) ;
  • 위승곤 (한국원자력연구소 방사선이용연구부) ;
  • 김재성 (한국원자력연구소 방사선이용연구부) ;
  • 이인중 (경북대학교 농학과)
  • Published : 2005.03.01
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Abstract

To investigate the synergistic effects of low dose gamma irradiation and growth regulators on the growth and photosynthesis in rice (Oryza sativa L.), laboratory and greenhouse experiments were conducted using 4-year-old rice seeds. In the laboratory experiment, the germination rate was increased in 0.001 ppm IBA treatment, showing the synergistic effect of gamma irradiation and growth regulators. The seedling growth was increased by treatment of GA₃ and IBA, the irradiated groups having higher than the non-irradiated ones. Particularly, it was remarkable in 0.001 ppm IBA. In greenhouse experiment, seedling growth was increased in response to a combination of gamma irradiation and 0.001 ppm IBA. Effective quantum yield of PSⅡ(Ф/sub PSⅡ/) and photochemical quenching (qP) were increased, while non-photochemical quenching (qN) was decreased by 0.001 ppm IBA. A synergistic effect of gamma irradiation and IBA was only found in seedling growth. The present results suggest that low dose gamma irradiation and growth regulator could synergistically stimulate seedling growth.

벼의 유묘 생육과 광합성 효율에 대한 저선량 방사선과 생장조절물질 간의 상승작용 효과를 확인하기 위해 4년 된 종자로 기내실험과 온실실험을 수행하였다. 기내실험에서 관찰한 종자 발아의 경우 방사선을 조사한 IBA 0.001 ppm 처리구에서 가장 높은 값을 보였다. 또한, 파종 6일 후 측정한 유묘 생육은 방사선 무 조사구보다 방사선을 조사한 GA₃와 IBA 처리구에서 대체로 높은 값을 보였으며, 그 중 IBA 0.001 ppm 처리에서 저선량 방사선과 생장조절물질간의 유의성 있는 상승작용 효과를 확인 할 수 있었다. 저선량 방사선을 조사 한 후 IBA 0.001 ppm을 처리하여 관찰한 온실 실험에서도 유묘 생육에서의 상승작용 효과를 확인할 수 있었다. 광합성 효율을 확인하기 위해 측정한 엽록소 형광은 생장조절물질에 의해서만 차이를 보여, IBA 처리에 의해 광계 2의 양자 수율을 나타내주는 Ф/sub PSⅡ/와 광화학적 소멸을 나타내는 qP는 증가하였고 비광화학적 소멸을 나타내는 형광지표인 qN은 감소하였다. 이러한 결과를 통해 저선량 방사선을 조사한 후 생장조절물질을 처리하였을 때 벼의 종자 발아와 더불어 생육이 두 요인간의 상승작용 효과로 촉진됨을 확인할 수 있었다.

Keywords

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