Characteristics of Soybean Hydrolysates Prepared with Various Protease

단백질 분해효소 종류에 따른 콩 가수분해물의 특성

  • Jeong, Kyu-Ho Jeong (Dept. of Food Science and Technology, Keimyung University and Keimyung Foodex Co.) ;
  • Seo, Ji-Hyung (Div. of Food, Berverage & Culinary Arts, Yeungnam College of Science & Technology) ;
  • Jeong, Yong-Jin (Dept. of Food Science and Technology, Keimyung University and Keimyung Foodex Co.)
  • 정규호 (계명대학교 식품가공학과 및 (주)계명푸덱스) ;
  • 서지형 (영남이공대학 식음료조리계열) ;
  • 정용진 (계명대학교 식품가공학과 및 (주)계명푸덱스)
  • Published : 2005.10.01

Abstract

In order to produce functional soy hydrolysates, we investigated the characteristics of soy hydrolysates prepared with 4 kinds of commercial proteases. The yield was high in protease(B), in which 43.2% soy flour and 61.6% SPI were obtained. The solubility and the contents of total phenolic compound were greatly increased by the treatment of protease(B) along with protease(C). The calcium intolerance was improved after the protease(B) treatment in soy flour or Soybean Protein isolate (SPI). Consideration for the physicochemical characteristics including yield, protease(B) has potential application for the production of soy hydrolysates. After the protease treatment, the beany flavor of soy flour became weak and the bitter taste was strong in both soy flour and SPI. However, there was no difference of beany flavor and bitter taste among delete protease hydrolysates. Nevertheless, further modifications and improvements to the sensory characteristics would be required for the development of a range of products with the hydrolysate.

콩 가수분해물의 기능성 강화를 위하여 콩분말 및 SPI에 4종의 protease를 처리하여 가수분해물의 특성을 조사하였다. 수율은 protease(B)를 처리하였을 때 콩분말에서 43.2%, SPI에서 61.6%로 가장 높게 나타났다. 용해도와 총페놀성 물질은 protease(B)와 (C)처리구에서 크게 증가하였으며, 칼슘내인성은 protease(B)를 처리하였을 때 향상되었다. 콩분말에서 콩 특유의 비린내는 protease처리로 감소하였으나 콩분말과 SPI 모두 protease 처리로 쓴맛이 강해졌으며 protease의 종류에 따른 차이는 확인되지 않았다. 가수분해물의 수율을 비롯한 이화학적 특성을 감안할 때, protease (B)가 가수분해물 제조에 적합하였으며, 가수분해물의 활용 형태에 따라 관능적 특성의 보완이 요구되었다.

Keywords

References

  1. Son, Y.S. and Kwon, T.W. (2000) Hypochlesterolemic effect of soybean and soy products. Food Industry and Nutrition, 5, 36-41
  2. Sung, M.K. (1996) The anticarcinogenic properties of soybeans. Korea Soybean Digest., 13, 19-31
  3. Tovar-Palacio, C., Potter, S.M., Hafermann, J.C. and Sahy, N.F. (1998) Intake of soy protein and soy protein extracts influences lipid metabolism and hepatic gene expression in gerbils. J. Nutr., 128, 839-842
  4. Kang, J.H. (1999) Funtional characterization of soy protein hydrolysate. Food Industry and Nutrition, 4, 66-72
  5. Jianping, W.U. and Xiaolin, D. (2002) Characterization of inhibition and stability of soy protein derived angiotensinI-converting enzyme inhibitory peptides. Food Res. Int., 35, 367-375 https://doi.org/10.1016/S0963-9969(01)00131-4
  6. Kim, C.S., Lee, Y.S., Kim, J.S. and Hahn, Y.H. (2000) High performance liquid chromatographic analysis of isoflavones in soybean foods. Korean J. Food Sci. Technol., 32, 25-30
  7. Liu, K. (2000) Expanding soybean food utilization. Food Technol., 54, 46-58
  8. Pratt, R., Dan, E., Pietro, W.L. and Giffee, J.W. (1981) Phenolic antioxidants of soy protein hydrolysate. J. Food Sci., 47, 24-31 https://doi.org/10.1111/j.1365-2621.1982.tb11018.x
  9. Health Industry Development Division. (2002) Report on 2001 national health and nutrition survey - Nutrition survey(I). Ministry Health & Welfare. p.155-222
  10. Chae, H.J., In, M.J. and Lee, J.D. (1998) Production of a protein supplement from soymilk residues by combined use of enzymes and microorganisms. Agri. Chem. Biotechnol., 41, 73-77
  11. Choung, N.H., Shin, Y.S., Kim, S.H. and Yim, M.H. (2003) Characteristics of soy protein hydrolysates with enzymes produced by microorganisms isolated from traditional Meju. Korean J. Food Preserv., 10, 80-88
  12. Pyun, J.W. and Hwang, I.K. (1995) Effects of protease treatment on functional properties of soymilk protein. Korean J. Soc. Food Sci., 11, 26-32
  13. Cha, M.H. and Yoon, S. (1993) Modification of functional properties of soy protein isolate by proteolytic enzymes. Korean J. Food. Sci. Technol., 25, 39-45
  14. Edwards, J.H. and Shipe, W.F. (1978) Characterization of plastein reaction products formed by pepsin, ${\alpha}-chymotrypsin$ and papain treatment of egg albumin hydrolysates. J. Food Sci., 43, 1215-1219 https://doi.org/10.1111/j.1365-2621.1978.tb15272.x
  15. A.O.A.C. 1980. Official methods of analysis. 13th ed., Association of Official Analytical Chemist. Washington, p.176-180
  16. Pyun, J.W. and Hwang, I.K. (1996) Preparation of calcium-fortified soymilk and in vitro digestion properties of its protein and calcium. Korean J. Food Sci. Technol., 28, 995-1000
  17. SAS. 1988. SAS/STAT User's Guide. Version 6 4th ed. SAS institute, Inc., Cary, NC
  18. Kim, S.Y., Park, P.S.. and Rhee, K.C. (1990) Functional properties of peroteolytic enzyme modified soy protein isolate. J. Agric. Food Chem., 38, 651-657 https://doi.org/10.1021/jf00093a014