Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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The Effects of Abscisic Acid Application Time and Times on Fruit Coloration of 'Kyoho' Grapes

Abscisic acid의 처리시기 및 횟수가 포도 '거봉'의 착색에 미치는 영향

  • Han Dong-Hyeon (Division of Bioscience and Technology, Korea University)
  • 한동현 (고려대학교 생명산업과학부)
  • Published : 2005.04.01
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Abstract

This experiment was carried to investigate the optimum time and times of abscisic acid (ABA) treatment for the coloration in 'Kyoho' grapes. The L-phenylalanine ammonia-lyase (PAL) enzyme activities were showed highly in both ABA treatments at veraison and 10 days after veraison. However, PAL enzyme was slightly higher in ABA treatment at 10 days after veraison than in that at veraison. Anthocyanin content showed a tendency that were increased during fruit development after veraison in all treatments, and was the highest in ABA treatment at 10 days after veraison. Fructose and glucose as soluble sugars were detected by HPLC and showed little differences in all treatments. In times of ABA treatment, PAL activity showed a tendency that decreased after increased in all treatments. PAL activity in 2 times treatment of ABA was higher than other treatments. Also, anthocyanin content was highest in 2 times treatment of ABA, as 5 folds of control and folds of 1 time treatment of ABA. Both fructose and glucose contents in all ABA treatments was slightly higher than control.

포도 '거봉'의 착색을 조장하기 위해 적절한 ABA의 처리시기와 처리 횟수를 알아보기 위하여 본 실험을 수행한 결과 다음과 같은 결과를 얻었다. PAL 효소의 활성은 변색기 처리구와 변색기 10일 후 처리구에서 활성이 높은 것으로 나타났으며, 변색기 10일후 처리구가 변색기 처리구보다 약간 더 높은 효소 활성을 나타냈다. 안토시아닌 함량은 각 처리구 공히 생육기간 동안 계속 증가하는 경향을 보였고, 모든 ABA 처리구는 무처리구에 비해 훨씬 높은 안토시아닌 함량을 나타내었으며, 변색기 10일후 처리구에서 가장 높은 안토시아닌 함량을 나타내었다. 가용성당 함량에 있어서 과당과 포도당만이 검출되었으며, 처리구간에 함량의 차가 그다지 크지 않았다. 처리 횟수에 있어서 PAL 효소의 활성은 각 처리구 공히 증가하다가 감소하는 경향을 보였으며, 2회 처리구는 변색기 10일후의 2회째 처리 이후에도 급속한 PAL 효소 활성 증가가 이어져 최종수확기에서도 비교적 상당히 높은 효소 활성을 보였다. 변색기와 변색기 10일 후의 2회 처리구에서 안토시아닌 함량 증가가 가장 높아 최종수확기에 가서는 무처리구에 비해 5배 이상, 1회 처리구에 비해서는 2배정도 많았다. 1회 및 2회 ABA 처리구 모두 과당과 포도당 함량 공히 무처리구에 비해 약간 높은 경향을 나타냈지만 그다지 큰 차이는 없었다.

Keywords

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