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The Pharmaceutical Society of Korea (대한약학회)
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Neuroprotective and Free Radical Scavenging Activities of Phenolic Compounds from Hovenia dulcis

  • Li, Gao (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology, College of Pharmacy, Yanbian University) ;
  • Min, Byung-Sun (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • Zheng, Chang-Ji (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology, College of Pharmacy, Yanbian University) ;
  • Lee, Joong-Ku (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh, Sei-Ryang (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn, Kyung-Seop (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Hyeong-Kyu (Laboratory of Immunomodulator, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2005.07.01
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Abstract

The EtOAc-soluble fraction from a methanolic extract of Hovenia dulcis Thunb. exhibited neuroprotective activity against glutamate-induced neurotoxicity in mouse hippocampal HT22 cells. The neuroprotective activity-guided isolation resulted in 8 phenolic compounds (1-8), such as vanillic acid (1), ferulic acid (2), 3,5-dihydroxystilbene (3), (+)-aromadendrin (4), methyl vanillate (5), (-)-catechin (6), 2,3,4-trihydrobenzoic acid (7), and (+)-afzelechin (8). Among these, compounds 6 and 8 had a neuroprotective effect on the glutamate-induced neurotoxicity in HT22 cells. Furthermore, compound 6 had a DPPH free radical scavenging effect with an $IC_{50}$ value of $57.7{\mu}M$, and a superoxide anion radical scavenging effect with an $IC_{50}$ value of $8.0{\mu}M$. Both compounds 6 and 8 had ABTS cation radical scavenging effects with $IC_{50}$ values of $7.8{\mu}M\;and\;23.7${\mu}M$, respectively. These results suggest that compounds 6 and 8 could be neuroprotectants owing to their free radical scavenging activities.

Keywords

References

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