Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Physiological Studies on Acute Water-temperature Stress of Juvenile Abalone, Haliotis discus hannai

급격한 수온 스트레스에 따른 전복, Haliotis discus hannai 치패의 생리학적 연구

  • Kim Tae-Hyung (Faculty of Applied Marine Science, Cheju National University) ;
  • Yang Moon-Hyu (Faculty of Applied Marine Science, Cheju National University) ;
  • Choe Mi-Kyung (Shellfish Genetic & Breeding Research Center) ;
  • Han Seok-Jung (Shellfish Genetic & Breeding Research Center) ;
  • Yeo In-Kyu (Faculty of Applied Marine Science, Cheju National University)
  • 김태형 (제주대학교 해양과학대학 해양과학부) ;
  • 양문휴 (제주대학교 해양과학대학 해양과학부) ;
  • 최미경 (국립수산과학원 패류육종연구센터) ;
  • 한석중 (국립수산과학원 패류육종연구센터) ;
  • 여인규 (제주대학교 해양과학대학 해양과학부)
  • Published : 2005.02.01
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Abstract

This study was conducted to investigate antioxidant enzyme activity (catalase and superoxide dismutase) and Heat Shock Protein 70 (HSP70) mRNA variation in hepatopancreas of abalone (Haliotis discus hannai) cultured under several acute water temperatures. Abalones were cultured at 10, 15, 20, 25 and $30^{\circ}C$, for 0, 6, 12, 24 and 48 hours, respectively. The HSP70 mRNA expression in hepatopancreas was more increased at $30^{\circ}C$ compared to those at 10. 15, 20 (control) and $25^{\circ}C$. The superoxide dismutase (SOD) activity was increased in hepato-pancreas at all water temperature conditions compared to the control ($20^{\circ}C$). The SOD activity at high water temperature (25 and $30^{\circ}C$) tended to be increased after 12 hours, and was increased immediately after exposure to low water temperature (10 and $15^{\circ}C$). and then was recovered to starting level after the increase. Also, catalase (CAT) activity in hepatopancreas was increased in all the groups except for at $10^{\circ}C$ than the control ($20^{\circ}C$). Survival rate of abalone was $100\%$ at 10, 15, 20 and $25^{\circ}C$, but $92\%$ at $30^{\circ}C$. Thus, according to our study, when abalone is appeared at $20^{\circ}C$, defense mechanism against stress at low water temperature can be accelerated to be stabilized at about $5^{\circ}C$. In the case of exposure of abalone to high water temperature, antioxidant enzyme and HSP70 expression were increased due to elevated physiological stimulation factor, such as temperature.

본 연구는 전복, Haliotis discus hannai 치패를 이용하여 급격한 수온변화 스트레스에 따른 간부위에서의 항산화효소 및 HSP70 mRNA의 변화를 조사하였다. 실험구는 10, 15, 20(대조구), 25 및 $30^{\circ}C$로 설정하였으며, 측정 시간은 0, 6, 12, 24 및 48h후에 측정하였다. 그 결과 HSP70 mRNA의 발현은 다른 실험구들과 비교하여 $30^{\circ}C$ 실험구에서만 유의적으로 높게 발현되었으며, SOD의 경우 모든 실험구에서는 증가하는 경향을 나타내었으나, 고수온에서 12 h 이후 급격한 상승을 나타내었고, 저수온에서는 수온 스트레스 자극 직후에 증가한 후 회복되는 경향을 나타내었다. CAT에서는 실험 개시 후부터 고수온 실험구에서 지속적인 상승을 나타내었다. 실험기간 중의 생존율은 $30^{\circ}C$ 실험구를 제외한 모든 실험구에서 $100\%$의 생존율을 나타내었으며, $30^{\circ}C$ 실험구에서는 $92\%$의 생존율을 나타내었다. 이상의 결과로, 20"C에서 순치된 전복은 저수온 스트레스에 대한 생리학적 방어기작이 약 $5^{\circ}C$ 내외의 고수온 스트레스에 대한 생리학적 방어기작과 비교하여 보다 빠르게 작용하여 안정화하는 것으로 나타났으며, 일정 수온 이상의 고수온에 노출되었을 경우에는 생체 내 과도한 생리학적 방어 기작이 작용하여 항산화효소 및 HSP70 발현이 증가하는 것으로 나타났다.

Keywords

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