Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Antimicrobial Effect of Cutellaria baicalensis George Extracts on Food-Borne Pathogens

황금(Cutellaria baicalensis George) 추출물의 식중독성 미생물에 대한 항균효과

  • Bae Ji-hyun (Department of Food Science and Nutrition, Keimyung University) ;
  • Lee Myung-Jin (Department of Food Science and Nutrition, Keimyung University) ;
  • Lee Sun-mi (Department of Food Science and Nutrition, Keimyung University)
  • 배지현 (계명대학교 식품영양학과) ;
  • 이명진 (계명대학교 식품영양학과) ;
  • 이선미 (계명대학교 식품영양학과)
  • Published : 2005.03.01
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Abstract

This study was performed to investigate the antimicrobial effect of the Cutellaria baicalensis George extracts against food-borne pathogens. First, the Cutellaria baicalensis George was extracted with methanol at room temperatures, and fractionation of the methanol extracts from Cutellaria baicalensis George was carried out by using petroleum ether, chloroform, and ethyl acetate, and methanol respectively. The antimicrobial activity of the Cutellaria baicalensis George extracts was determined using a paper disc method against food-borne pathogens and food spoilage bacteria. The ethyl acetate extracts of Cutellaria baicalensis George showed the highest antimicrobial activity against Staphylococcus aureus and Shigella dysenteriae. The synergistic effect has been found in combined extracts of Cutellaria baicalensis George and Portulaca oleracea as compared to each extracts alone. Finally, the growth inhibition curve was determined using ethyl acetate extracts of Cutellaria baicalensis George against Staphylococcus aureus and Shigella dysenteriae. The ethyl acetate extract of Cutellaria baicalensis George showed strong antimicrobial activity against Staphylococcus aureus at the concentration of 4,000 ppm. The 4,000 ppm of ethyl acetate extract from Cutellaria baicalensis George retarded the growth of S. aureus more than 24 hours and Shigella dysenteriae up to 36 hours. The ethyl acetate extracts of Cutellaria baicalensis George has been shown the antimicrobial effect against Staphylococcus aureus and Shigella dysenteriae.

본 연구에서는 식중독 유발세균에 대한 항균활성이 우수한 천연 항균성 물질을 검색하기 위해 예로부터 민간과 한방에서 널리 이용되어 온 황금을 각종 유기용매로 추출하여 식중독 유발세균에 대한 항균활성을 조사해 보았다. 황금을 methanol로 추출한 후, petroleum ether, chloroform, ethyl acetate를 이용하여 실온에서 각각 용매별로 계통 분획하고, 열수추출물을 얻은 후, 9종의 식중독 유발세균(Staphylococcus aureus, Bacillus cereus, Salmonella enteritidis, Shigella flexneri, Escherichia coli, Salmonella typhimurium, Shigella dysenteriae, Pseudomonas aruginosa, Shigella sonnei) 에 대하여 항균효과를 조사하였다. 황금 추i물의 농도별 항균 활성 검색에서는 황금의 ethyl acetate추출물이 가장 큰 항균 효과를 보였으며 S. aureus와 S. dysenteriae가 가장 민감하게 반응하는 균주였다. 황금의 ethyl acetate추출물과 사설초의 ethyl acetate 추출물을 혼합하여 항균력을 측정해 본 결과 두 추출물을 섞어 첨가했을 경우가 단독으로 사용했을 때 보다 상승효과를 나타내었다 또한 황금의 ethyl acetate추출물이 식중독 유발세균의 성장에 미치는 효과를 검정하기 위해 S. aureus및 S. dysenteriae의 배양백에 황금의 ethyl acetate 추물을 각각 5,000 ppm 농도로 첨가했을 때, S. aureus의 생육이 24시간 이상까지 억제됨을 관찰할 수 있었고, S. dysenteriae의 생육도 36시간까지 지연시킬 수 있었다. 이상의 결과 황금의 ethyl acetate추출물은 S. aureus와 S. dysenteriae의 성장을 억제하는 것을 알 수 있었다.

Keywords

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