Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
권호 표지
DOI QR코드

DOI QR Code

The Amount of Telomeres and Telomerase Activity on Chicken Embryonic Cells During Developmental Stages

닭의 발생 단계별 세포내 Telomere의 양적 분포양상과 Telomerase 활성도 분석

  • Cho, E.J. (Department of Animal Science and Technology & RAIRC, Jinju National University) ;
  • Choi, C.H. (National Livestock Research Institute, RDA) ;
  • Sohn, S.H. (Department of Animal Science and Technology & RAIRC, Jinju National University)
  • 조은정 (진주산업대학교 동물생명과학과.동물생명산업연구센터) ;
  • 최철환 (농진청 축산연구소) ;
  • 손시환 (진주산업대학교 동물생명과학과.동물생명산업연구센터)
  • Published : 2005.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Telomeres locate at the end of chromosomes and consist of a tandem repeat sequence of $(TIAGGG)^{n}$ and associated proteins. Telomerase is a ribonucleoprotein which act as a template for the synthesis of telomeric DNA. Telomeres are essential for chromosome stability and are related with cell senescence, apoptosis and cancer. This study was carried out to analyze the amount of telomeres and telomerase activity of chicken cells during embryonic and developmental stages. The whole embryos and prenatal tissues such as brain, heart, liver, kidney and testis at different developmental stages were obtained from Korean Native Chicken. The amount of telomeres on embryonic cells was analyzed by quantitative fluorescence in situ hybridization (Q-FISH) techniques using the chicken telomeric DNA probe. Telomerase activity was measured by telomeric repeat amplification protocol (TRAP) assay. Results indicated that the amounts of telomeric DNA on the most embryonic cells were gradually decreased during ontogenesis. Furthermore, the quantity of telomeres was quite different among embryonic tissues according to developmental origin. The relative amount of telomeres has more in regenerative cells such as embryonic disc and testicular cells than in non-regenerative cells such as liver, brain, heart and kidney cells. Telomerase activity was also highly detectable in most chicken cells at early embryonic stages. After 9 days of incubation, however, the telomerase activitie W

Keywords

References

  1. Ahmed, A. and Tollefsbol, T. 2001. Telomeres and telomerase: basic science implications for aging. J. Am. Geriatr. Soc. 49(8):1105-1109 https://doi.org/10.1046/j.1532-5415.2001.49217.x
  2. Bekaert, S., Derradji, H. and Baatout, S. 2004. Telomere biology in mammalian germ cells and during development. Dev. Biol. 274(1):15-30 https://doi.org/10.1016/j.ydbio.2004.06.023
  3. Betts, D. H. and King, W. A. 1999. Telomerase activity and telomere detection during early bovine development. Dev. Genet. 25(4):397-403 https://doi.org/10.1002/(SICI)1520-6408(1999)25:4<397::AID-DVG13>3.0.CO;2-J
  4. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anim. Biochem. 72:248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  5. Byant, J. E., Hutchings, K. G., Moyzis, R. K. and Griffith, J. K. 1997. Measurement of telomeric DNA content in human tissues. Biotech. 23:476-478
  6. Cottliar, A. S. and Slavutsky, I. R. 2001. Telomeres and telomerase activity: their role in aging and in neoplastic development. Medicina 61(3):335-342
  7. Delany, M. E., Daniels, L. M., Swanberg, S. E. and Taylor, H. A. 2003. Telomeres in the chicken: genome stability and chromosome ends. Poul. Sci. 82:917-926
  8. Ferlicot, S., Youssef, N., Feneux, D., Delhommeau, F., Paradis, V. and Bedossa, P. 2003. Measurement of telomere length on tissue sections using quantitative fluorescence in situ hybridization(Q-FISH). J. Path. 200:661-666 https://doi.org/10.1002/path.1392
  9. Forsyth, N. R., Wright, W. E. and Shay, J. W. 2002. Telomerase and differentiation in multicellular organisms: Turn it off, turn it on, and turn it off again. Differentiation 69:188-197 https://doi.org/10.1046/j.1432-0436.2002.690412.x
  10. Gan, Y., Engelke, K. J., Brown, C. A. and Au, J. L. 2001. Telomere amount and length assay. Pharm. Res. 18(12):1655-1659 https://doi.org/10.1023/A:1013306109801
  11. Greider, C. W. and Blackburn, E. H. 1985. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell 43:405-413 https://doi.org/10.1016/0092-8674(85)90170-9
  12. Hultdin, M., Gronlund, E., Norrback, K., Erikasson- Lindstrom, E., Just, T. and Roos, G. 1998. Telomere analysis by fluorescence in situ hybridization and flow cytometry. Nucleic Acids Res. 26(16):3651-3656 https://doi.org/10.1093/nar/26.16.3651
  13. Kim, N. W. and Wu, F. 1997. Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol(TRAP). Nucleic Acids Res. 25(13):2595-2597 https://doi.org/10.1093/nar/25.13.2595
  14. Kobayashi, J., Sekimoto, A., Uchida, H., Wada, T., Sasaki, K., Sasada, H., Umezu, M. and Sato, E. 1998. Rapid detection of male-specific DNA sequence in bovine embryos using fluorescence in situ hybridization. Mol. Reprod. Dev. 51:390-394 https://doi.org/10.1002/(SICI)1098-2795(199812)51:4<390::AID-MRD5>3.0.CO;2-F
  15. Lejnine, S., Vladimir, L. and Langmore, J. P. 1995. Conserved nucleoprotein structure at the ends of vertebrate and invertebrate chromosomes. Proc. Natl. Acad. Sci. USA 92:2393-2397 https://doi.org/10.1073/pnas.92.6.2393
  16. Meeker, A. K. and Coffey, D. S. 1997. Telomerase: a promising marker of biological immortality of germ, stem, and cancer cells. A review. Biochem. (Mosc). 62(11):1323-1331
  17. Oexle, K. 1998. Telomere length distribution and southern blot analysis. J. Theor. Biol. 190:369-377 https://doi.org/10.1006/jtbi.1997.0559
  18. Pathak, S., Mutani, A. S., Furlong, C. L. and Sohn, S. H. 2002. Telomere dynamics, aneuploidy, stem cells and cancer. Int. J. Oncology 20(3):637- 641
  19. Poon, S. S., Martens, U. M., Ward, R. K. and Lansdorp, P. M. 1999. Telomere length measurements using digital fluorescence microscopy. Cytometry 36:267-278 https://doi.org/10.1002/(SICI)1097-0320(19990801)36:4<267::AID-CYTO1>3.0.CO;2-O
  20. Robinson, M. O. 2000. Telomere and cancer. Genet. Eng. 22:209-222
  21. Rufer, N., Dragowska, W., Thornbury, G., Roosnek, E. and Lansdorp, P. M. 1998. Telomere length dynamics in human lymphocyte subpopulations measured by flow cytometry. Nat. Biotechnol. 16(8):743-747 https://doi.org/10.1038/nbt0898-743
  22. Slijepcevic, P. 2001. Telomere length measurement by Q-FISH. Cell Sci. 23:17-22 https://doi.org/10.1023/A:1013177128297
  23. Sohn, S. H., Multani, A. S., Gugnani, P. K. and Pathak, S. 2002. Telomere erosion-induced mitotic catastrophe in continuously grown Chinese hamster Don cells. Exptl. Cell Res. 279(2):271-276 https://doi.org/10.1006/excr.2002.5614
  24. Taylor, H. A. and Delany, M. E. 2000. Ontogeny of telomerase in chicken: Impact of downregulation on pre- and postnatal telomere length in vivo. Dev. Growth Differ. 42:613-621 https://doi.org/10.1046/j.1440-169x.2000.00540.x
  25. Ulaner, G. A., Hu, J. F., Vu, T. H. and Hoffman, A. R. 1998. Telomerase activity in human development is regulated by human telomerase reverse transcriptase (hTERT) transcription and by alternate splicing of hTERT transcripts. Cancer Res. 58:4168-4172
  26. Ulaner, G. A., Hu, J. F., Vu, T. H. and Hoffman, A. R. 2001. Tissue-specific alternate splicing of human telomerase reverse transcriptase(hTERT) influences telomere lengths during human development. Int. J. Cancer 91:644-649 https://doi.org/10.1002/1097-0215(200002)9999:9999<::AID-IJC1103>3.0.CO;2-V
  27. Venkatesan, R. and Price, C. 1998. Telomerase expression in chickens: Constitutive activity in somatic tissues and down- regulation in culture. Proc. Natl. Acad. Sci. USA 95:14763-14768 https://doi.org/10.1073/pnas.95.25.14763
  28. Wright, D. L., Jones, E. L. Mayer, J. F., Oehninger, S., Gibbons, W. E. and Lanzendorf, S. E. 2001. Characterization of telomerase activity in the human oocyte and preimplantation embryo. Mol. Hum. Reprod. 7:947-955 https://doi.org/10.1093/molehr/7.10.947
  29. Wright, W. E., Piatyszek, M. A., Rainey, W. E.. Byrd, W. and Shay, J. W. 1996. Telomerase activity in human germline and embryonic tissues and cells. Dev. Genet. 18:173-179 https://doi.org/10.1002/(SICI)1520-6408(1996)18:2<173::AID-DVG10>3.0.CO;2-3
  30. Xu, J. and Yang, X. 2000. Telomerase activity in bovine embryos during early development. Biol. Reprod. 63(4):1124-1128 https://doi.org/10.1095/biolreprod63.4.1124

Cited by

  1. Dynamics of telomere length in the chicken vol.70, pp.04, 2014, https://doi.org/10.1017/S0043933914000804