Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Analysis and Selection of Microsatellites Markers for Individual Traceability System in Hanwoo

한우 생산이력제에 활용 가능한 Microsatellite의 분석과 선발

  • Lim, H.T. (Division of Applied Life Science, Gyeongsang National University) ;
  • Min, H.S. (Division of Applied Life Science, Gyeongsang National University) ;
  • Moon, W.G. (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, J.B. (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, J.H. (Division of Applied Life Science, Gyeongsang National University) ;
  • Cho, I.C. (National Institute of Subtropical Agriculture, R. D. A.) ;
  • Lee, H.K. (Genetic Informatics Center, Hankeong National University) ;
  • Lee, Y.W. (GenDocs Cooperation) ;
  • Lee, J.G. (Division of Applied Life Science, Gyeongsang National University) ;
  • Jeon, J.T. (Division of Applied Life Science, Gyeongsang National University)
  • 임현태 (경상대학교 응용생명과학부) ;
  • 민희식 (경상대학교 응용생명과학부) ;
  • 문원곤 (경상대학교 응용생명과학부) ;
  • 이재봉 (경상대학교 응용생명과학부) ;
  • 김재환 (경상대학교 응용생명과학부) ;
  • 조인철 (농촌진흥청 난지농업연구소) ;
  • 이학교 (한경대학교 유전정보연구소) ;
  • 이용욱 ((주)젠닥스) ;
  • 이정규 (경상대학교 응용생명과학부) ;
  • 전진태 (경상대학교 응용생명과학부)
  • Published : 2005.08.31
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Abstract

To test applicability to the Hanwoo traceability system, twenty microsatellite markers were selected and analyzed. MSA, CERVUS, FSTAT, GENEPOP, API_CALC and PHYLIP software was employed serially to estimate heterozygosity, polymorphic information content, F-statistics, identity probability, exclusion probability and genetic distance. Eleven microsatellite markers(TGLA53, TGLA227, ETH185, TGLA122, BM4305, INRA23, ILSTS013, BMS1747, BM2113, BL1009, and ETH3) were selected based on their high heterozygosity values. Identity probability using these markers is one hundred times higher than when using StockMakersTM of Applied Biosystems. This indicates the selected microsatellite markers are appropriate and effective for use in the Hanwoo traceability system. Additionally, estimates of DA genetic distance and pairwise-FST can be utilized to identify genetic relationships between adjacent farms.

한우의 생산이력제에 활용 가능한 20종의 microsatellite marker를 선정하고 다형성지수, F-통계량, 동일개체 출현확률, 친자감별 확률 및 유전적 거리지수 등을 MSA, CERVUS, FSTAT, GENEPOP, API-CALC 및 PHYLIP 프로그램 등을 연계적으로 활용하여 추정하였다. Heter- ozygosity 추정치에 근거하여 선발한 11개의 microsatellite(TGLA53, TGLA227, ETH185, TGLA122, BM4305, INRA23, ILSTS013, BMS1747, BM2113, BM2113, BL1009와 ETH3)는 Applied Biosystems사의 StockMakersTM와 비교하여 100배 정도의 동일개체 출현확률이 낮아 한우의 생산이력제 적용에 보다 효율적인 것으로 나타났다. 또한 DA 유전적 거리지수와 pairwise-FST 추정치를 활용하여 근접지역의 농장간 근연관계의 정도를 파악할 수 있는 자료로 활용할 수 있을 것으로 사료된다.

Keywords

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