Variation of Isoflavone Contents in Korean Soybean Germplasms

국내 콩 유전자원의 Isoflavone 함량 변이

  • Choung, Myoung-Gun (Department of Pharmacognosy Material Development, Kangwon National University) ;
  • Kang, Sung-Taeg (National Institute of Crop Science, RDA) ;
  • Han, Won-Young (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Baek, In-Youl (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Kim, Hyeun-Kyeung (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Shin, Doo-Chull (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Kang, Nam-Suk (Yeongnam Agricultural Research Institute, NICS, RDA) ;
  • Hwang, Young-Sun (Department of Pharmacognosy Material Development, Kangwon National University) ;
  • An, Young-Nam (Department of Pharmacognosy Material Development, Kangwon National University) ;
  • Lim, Jung-Dae (Department of Pharmacognosy Material Development, Kangwon National University) ;
  • Kim, Kwan-Su (Department of Medicinal Plant Resources, Mokpo National University) ;
  • Park, Si-Hyung (Department of Medicinal Plant Resources, Mokpo National University) ;
  • Kim, Sun-Lim (National Institute of Crop Science, RDA)
  • 정명근 (강원대학교 생약자원개발학과) ;
  • 강성택 (작물과학원) ;
  • 한원영 (작물과학원 영남농업연구소) ;
  • 백인열 (작물과학원 영남농업연구소) ;
  • 김현경 (작물과학원 영남농업연구소) ;
  • 신두철 (작물과학원 영남농업연구소) ;
  • 강남숙 (작물과학원 영남농업연구소) ;
  • 황영선 (강원대학교 생약자원개발학과) ;
  • 안영남 (강원대학교 생약자원개발학과) ;
  • 임정대 (강원대학교 생약자원개발학과) ;
  • 김관수 (목포대학교 생약자원학과) ;
  • 박시형 (목포대학교 생약자원학과) ;
  • 김선림 (작물과학원)
  • Published : 2006.11.30

Abstract

Isoflavone in soybean is known to have the important physiological functions such as antioxidative, estrogenic, and anti-tumor activities. Therefore, isoflavone content is considered as important aspect of soybean quality. The objectives of this study are to determine the isoflavone contents of soybean germplasms including major cultivars in Korea, and to provide the basic information of isoflavone far development soybean cultivar containing high isoflavone content. Among 43 Korean major cultivated soybean samples, the total isoflavone contents were varied from $277.0{\mu}g/g\;to\;2,002.0{\mu}g/g$, and the average total isoflavone content was $1,017.3{\pm}390.0{\mu}g/g$. Total isoflavone content of Seokryangputkong $(2,002{\mu}g/g)$ was the highest among those of the other cultivated soybeans. On the other side, Hwangkeumkong $(277{\mu}g/g)$ contained lower isoflavone than other cultivars. The total isoflavone content of vegetable use or early maturity soybean cultivar $(1,438{\pm}493.7{\mu}g/g)$ showed more higher total isoflavone content than others. The daidzein, glycitein, genistein and total isoflavone contents of 648 soybean germplasms collected in Korea, were ranged $16.9{\sim}2,019.8{\mu}g/g,\;0{\sim}294.9{\mu}g/g,\;38.5\sim1,337.7{\mu}g/g,\;and\;82.6{\sim}3,479.2{\mu}g/g$, respectively, and total isoflavone content of IT 180001 line $(3,479.2{\mu}g/g)$ was the highest among those of other soybean germplasm lines.

고 isoflavone 함유 콩 유전자원 선발 및 품종육성의 기초자료로 활용하기 위해 국내 콩 주요품종 43계통 및 보존 유전자원 648계통의 isoflavone 함량을 분석한 결과를 요약하면 다음과 같다. 1. 한국산 콩 주요 43품종의 평균 isoflavone 함량은 daidzein이 $484.4{\pm}223.9{\mu}g/g$, genisteinol $448.0{\pm}156.2{\mu}g/g$, glyciteinol $84.9{\pm}48.7{\mu}g/g$, total isoflavoneol $1,017.3{\pm}390.0{\mu}g/g$을 나타내었다. 2. 공시된 주요 품종의 isoflavone 함량은 석양풋콩이 $2,002{\mu}g/g$으로 가장 높은 함량을 나타내었고, 황금콩이 $277{\mu}g/g$으로 가장 낮은 함량을 나타내었다. 또한 콩 주요 43품종을 용도별로 분류하여 isoflavone 함량을 비교한 결과 풋콩 및 올콩이 $1,438{\pm}493.7{\mu}g/g$을 나타내어 조사된 용도별 콩 분류 중 가장 높은 양상을 나타내었다. 3. 국내 보존 콩 유전자원 648계통을 대상으로 isoflavone 함량을 조사한 결과 IT 180001계통이 $3,479{\mu}g/g$의 함량을 나타내어 가장 높은 양상을 나타내었고, IT 910878 계통은 $82.61{\mu}g/g$의 total isoflavone을 함유하여 가장 낮은 계통으로 조사되어 보존 유전자원간에 큰 함량변이를 나타내었다.

Keywords