KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Effect of Polyamines on Indigo Biosynthesis in Hairy Root Cultures of Polygonum tinctorium Lour.

Polyamine이 쪽 모상근배양에서 인디고 생합성에 미치는 영향

  • Kim, Jin-Nam (School of Biological Sciences and Technology, Chonnam National University) ;
  • Jang, Hong-Gi (SENOCO Co.) ;
  • Park, Sang-Un (Division of Plant Science and Resources, Chungnam National University) ;
  • Ryu, Hwa-Won (School of Biological Sciences and Technology, Chonnam National University)
  • Published : 2006.11.30
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Abstract

We herein studied the effect of polyamines (putrescine, spermidine and spermine) on growth and indigo biosynthesis in hairy root cultures of Polygonum tinctorium Lour. Our results revealed that polyamine treatment increased hairy root growth and indigo biosynthesisat all tested concentrations, with the highest growth rate (4.4 g/ flask) and indigo yield (216 ug/g) induced by 70 mg/L putrescine. These results show far the first time that the growth rates and indigo biosynthesis of Polygonum tinctorium hairy roots may be improved by addition of polyamines to the liquid culture medium.

본 연구는 polyamine이 쪽 모상근 배양에서 뿌리의 생장과 indigo 생합성에 미치는 영향을 조사하였다. 1. 토양미생물인 Agrobacterium rhizogenes R1000을 이용하여 쪽 자엽으로부터 모상근을 효과적으로 유도하여 배양하였다. 2. 모상근 생장과 인디고 생합성이 polyamines(putrescine, spaermidine, spermine) 처리에 의하여 향상되었으며, 그 중 putrescine이 다른 polyamines에 비하여 효과적이었다. 3. Putrescine 70 mg/l 처리가 모상근의 생육(4.4 g/flask)과 인디고 생합성(216.3 ug/g)의 향상에 최적조건으로 조사되었다.

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