Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Post Feeding Trypsin Activity in the Digestive Organs and the Gastric Evacuation Rate of Litopenaeus vannamei (Boone)

먹이섭취 후 흰다리새우, Litopenaeus vannamei (Boone) 소화기관의 trypsin 활성 및 배설률

  • Kim, Su-Kyoung (Crustacean Research Center, Notional Fisheries Research and Development Institute) ;
  • Kim, Dae-Hyun (Crustacean Research Center, Notional Fisheries Research and Development Institute) ;
  • Kim, Bong-Rae (Crustacean Research Center, Notional Fisheries Research and Development Institute) ;
  • Kim, Jong-Seek (Crustacean Research Center, Notional Fisheries Research and Development Institute) ;
  • Cho, Yeong-Rok (Crustacean Research Center, Notional Fisheries Research and Development Institute) ;
  • Seo, Hyung-Cheol (Crustacean Research Center, Notional Fisheries Research and Development Institute) ;
  • Kim, Jong-Hwa (Crustacean Research Center, Notional Fisheries Research and Development Institute) ;
  • Han, Chang-Hee (Dong Eui University) ;
  • Jang, In-Kwon (West Sea Fisheries Research Institute, National Fisheries Research and Development Institute)
  • 김수경 (국립수산과학원 서해수산연구소 갑각류연구센터) ;
  • 김대현 (국립수산과학원 서해수산연구소 갑각류연구센터) ;
  • 김봉래 (국립수산과학원 서해수산연구소 갑각류연구센터) ;
  • 김종식 (국립수산과학원 서해수산연구소 갑각류연구센터) ;
  • 조영록 (국립수산과학원 서해수산연구소 갑각류연구센터) ;
  • 서형철 (국립수산과학원 서해수산연구소 갑각류연구센터) ;
  • 김종화 (국립수산과학원 서해수산연구소 갑각류연구센터) ;
  • 한창희 (동의대학교) ;
  • 장인권 (국립수산과학원 서해수산연구소)
  • Published : 2006.02.25
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Abstract

The tryptic enzyme activities from hepatopancreas, foregut, midgut and feces were examined to optimize the feeding method in whiteleg shrimp, Litopenaeus vannamei. The highest tryptic enzyme activity was found in hepatopancreas. The enzyme activities of hepatopancreas were 4 times higher than those of foregut per mg dry weight at 30 minutes feeding. Post feeding period, the activities of hepatopancreas increased continuously up to 30 hours after feeding. Trypsin activities of foregut showed about 3 times higher than did those of midgut. Average activity of foregut reached the pick with $303{\pm}68\;(mean{\pm}SE)$ nmol/mg/min at two hours after feeding and kept the activity up to 4 hours after feeding and thereafter the activity decreased. Average tryptic enzyme activity of midgut increased to $96{\pm}26nmol/mg/min$ up to two hours after feeding and it decreased to $52{\pm}17nmol/mg/min$ at five hours after feeding eventhough the gastric evacuation rate was still 50% by then. Foregut clearance occurred in 30 minutes after feeding and midgut weight increased up to 2 hours after feeding. Also we found that the maximal food ingestion in foregut was equivalent to the average 0.3% of its body weight by 30 minutes after feeding. Up to 5 hours after feeding, the weight ratio of midgut to body weight reduced, but still the weight ratio of foregut to body weight kept the similarity until then. These indicated that the tryptic enzyme activity and the clearance rate are different among the digestive organs and between forgot and midgut during the post feeding period in whiteleg shrimp.

흰다리새우 양식기법의 최적화를 위하여 소화기간 동안의 간췌장, 전장 그리고 중장에서의 trypsin의 활성변화를 조사하였다. 체중에 대한 전장, 중장의 무게 그리고 그 무게 합의 비율은 섭취된 먹이의 이동 및 소화과정을 나타내는 지표로서 공급량과 잔류량에 의한 먹이섭취량을 측정하는 것 보다 더 정확한 지표로서 사용 가능하였다. 평균적으로 치대 먹이섭취량은 전장에서 먹이섭취 후 30분 이내에 체중에 0.3%로 나타났다. 또한 30분 이후부터 전장이 비워지기 시작되었으며 중장의 무게가 최대에 이르는 시각은 2시간째였다. $3{\sim}5$시간 후에는 먹이가 배설됨으로 인하여 중장의 체중에 대한 무게비가 감소하였으나 전장에서는 비교적 같은 비율을 유지하였다. 먹이섭취에 의한 trypsin활성변화는 간췌장에서 가장 커서 전장에서의 활성변화에 비하여 약 3배로 나타났다. 소화시간이 지날수록 간췌장에서의 trypsin 활성은 지속적으로 증가하였다. 전장에서의 trypsin 효소의 활성은 중장보다 약 $2{\sim}3$배정도 높았다 먹이섭취 후 2시간이 지났을 때 trypsin 활성은 303 n mol/mg/min였고, 4시간까지 이 활성이 유지($277{\sim}306$ n mol/mg/min)되었으며, 그 후에는 점차 감소하였다. 중장에서는 typsin 활성이 먹이를 섭취하여 한 시간이 지나면서 $65{\pm}29$ (SE) n mol/mg/min로 증가하였다. 그 이후에는 $80{\sim}97$ n mol/mg/min의 범위를 나타내었으며, 5시간이 경과하였을 때 $52{\pm}17$ (SE) n mol/mg/min로 감소하였고 소화관내에 잔류하고 있는 먹이 량은 최대 섭취량의 50%로 나타났다.

Keywords

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