Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Inhibition of Chronic Skin Inflammation by Topical Anti-inflammatory Flavonoid Preparation, Ato $Formula^{\circledR}$

  • Lim, Hyun (College of Pharmacy, Kangwon National University) ;
  • Son, Kun-Ho (Dept. Food and Nutrition, Andong National University) ;
  • Chang, Hyeun-Wook (College of Pharmacy, Yeungnam University) ;
  • Kang, Sam-Sik (Natural Products Research Institute, Seoul National University) ;
  • Kim, Hyun-Pyo (College of Pharmacy, Kangwon National University)
  • Published : 2006.06.01
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Abstract

Flavonoids are known as natural anti-inflammatory agents. In this investigation, an anti-inflammatory potential of new topical preparation (SK Ato $Formula^{\circledR}$) containing flavonoid mixtures from Scutellaria baicalensis Georgi roots and Ginkgo biloba L. leaves with an extract of Gentiana scabra Bunge roots was evaluated in an animal model of chronic skin inflammation. Multiple 12-O-tetradecanoylphorbol-13-acetate treatments for 7 consecutive days on ICR mouse ear provoked a chronic type of skin inflammation: dermal edema, epidermal hyperplasia and infiltration of inflammatory cells. When topically applied in this model, this row formulation $(5-20\;{\mu}L/ear/treatment)$ reduced these responses. Furthermore, it inhibited prostaglandin $E_2$ generation (17.1-33.3%) and suppressed the expression of proinflammatory genes, cyclooxygenase-2 and $interleulin-1{\beta}$ in the skin lesion. Although the potency of inhibition was lower than that of prednisolone, all these results suggest that Ato $Formula^{\circledR}$ may be beneficial for treating chronic skin inflammatory disorders such as atopic dermatitis.

Keywords

References

  1. Andersen, S., Sjursen, W., Laegreid, A., Volden, G., and Johansen, B., Elevated expression of human nonpancreatic phospholipase A2 in psoriatic tissue. Inflammation, 18, 1-12 (1994) https://doi.org/10.1007/BF01534593
  2. Chang, H. W., Baek, S. H., Chung, K. W., Son, K. H., Kim, H. P., and Kang, S. S., Inactivation of phospholipase A2 by naturally occurring biflavonoid, ochnaflavone. Biochem. Biophys. Res. Comm., 205, 843-849 (1994) https://doi.org/10.1006/bbrc.1994.2741
  3. Chi, Y. S., Cheon, B. S., and Kim, H. P., Effect of wogonin, a plant flavone from Scutellaria radix, on the suppression of cyclooxygenase and the induction of inducible nitric oxide synthase in lipopolysaccharide-treated RAW 264.7 cells. Biochem. Pharmacol., 61, 1195-1203 (2001a) https://doi.org/10.1016/S0006-2952(01)00597-4
  4. Chi, Y. S., Jong, H., Son, K. H., Chang, H. W., Kang, S. S., and Kim, H. P., Effects of naturally occurring prenylated flavonoids on arachidonic acid metabolizing enzymes: cyclooxygenases and lipoxygenases. Biochem. Pharmacol., 62, 185-191 (2001b)
  5. Chi, Y. S., Lim, H., Park, H., and Kim, H. P., Effect of wogonin, a plant flavone from Scutellaria radix, on skin inflammation: in vivo regulation of inflammation-associated gene expression. Biochem. Pharmacol., 66, 1271-1278 (2003) https://doi.org/10.1016/S0006-2952(03)00463-5
  6. Fogh, K., and Kragballe, K., Eicosanoids in inflammatory skin diseases. Prostag. Other Lipid Mediators., 63, 43-54 (2000) https://doi.org/10.1016/S0090-6980(00)00096-4
  7. Gil, B., Sanz, M. J., Terencio, M. C., Gunasegaran, R., Paya, M., and Alcaraz, M. J., Morelloflavone, a novel biflavonoid inhibitor of human secretory phospholipase A2 with antiinflammatory activity. Biochem. Pharmacol., 53, 733-740 (1997) https://doi.org/10.1016/S0006-2952(96)00773-3
  8. Jensen, S. R. and Schripsema, J., Chemotaxonomy and pharmacology of Gentianaceae : in Gentianaceae-Systematics and Natural History, Struwe, L. and Albert, V. eds., Cambridge University Press, UK, pp. 573-631 (2002)
  9. Kim, H. P., Pham, H. T., and Ziboh, V. A., Flavonoids differentially inhibit guinea pig epidermal cytosolic phospholipase A2. Prostag. Leukot. Essen. Fatty Acids, 65, 281-286 (2001) https://doi.org/10.1054/plef.2001.0326
  10. Kim, H. P., Son, K. H., Chang, H. W., and Kang, S. S., Antiinflammatory plant flavonoids and cellular action mechanisms. J. Pharmacol. Sci., 96, 229-245 (2004) https://doi.org/10.1254/jphs.CRJ04003X
  11. Kwak, W-J., Kim, J-H., Ryu, K-H., Cho Y-B., Jeon, S-D., and Moon, C-K., Effects of gentianine on the production of proinflammatory cytokines in male Sprague-Dawley rats treated with lipopolysaccharide (LPS). Biol. Pharm. Bull., 28, 750-753 (2005) https://doi.org/10.1248/bpb.28.750
  12. LaDucca, J. R. and Caspari, A. A., Targeting tumor necrosis factor alpha. New drugs used modulate inflammatory diseases. Dermatol. Clin., 19, 617-635 (2001) https://doi.org/10.1016/S0733-8635(05)70304-1
  13. Middleton, E., Kandaswami, C., and Theoharides, T. C., The effects of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol. Rev., 52, 673-751 (2000)
  14. Natural Product Research Institute, Seoul National University, Medicinal Plants in the Republic of Korea, World Health Organization, Manila p. 131 (1998)
  15. Stanley, P. L., Steiner, S., Havens, M., and Tramposch, K. M., Mouse skin inflammation induced by multiple topical applications of 12-O-tetradecanoylphorbol-13-acetate. Skin Pharmacol., 4, 262-271 (1991) https://doi.org/10.1159/000210960