The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Collection, Identification and Hepatic Effect of Native Cordyceps militaris

새로운 번데기 동충하초의 수집, 동정 및 간기능에 미치는 효과

  • Lee, Ki-Won (Dong-A University Medical Science Research Center, Clinical Research Centery) ;
  • Nam, Byung-Hyouk (Dong-A University Medical Science Research Center, Clinical Research Centery) ;
  • Jo, Wool-Soon (Dong-A University Medical Science Research Center, Clinical Research Centery) ;
  • Oh, Su-Jung (Dong-A University Medical Science Research Center, Clinical Research Centery) ;
  • Kang, Eun-Young (Dong-A University Medical Science Research Center, Clinical Research Centery) ;
  • Cui, Yong (Dong-A University Medical Science Research Center, Clinical Research Centery) ;
  • Lee, Jae-Yun (Department of Microbiology, College of Natural Sciences, Pusan National University) ;
  • Cheon, Sang-Cheol (Institute for Bioindustry) ;
  • Jeong, Min-Ho (Dong-A University Medical Science Research Center, Clinical Research Centery) ;
  • Lee, Jae-Dong (Department of Microbiology, College of Natural Sciences, Pusan National University)
  • 이기원 (동아대학교 의과학연구원, 동아대학교병원 임상시험연구센터) ;
  • 남병혁 (동아대학교 의과학연구원, 동아대학교병원 임상시험연구센터) ;
  • 조월순 (동아대학교 의과학연구원, 동아대학교병원 임상시험연구센터) ;
  • 오수정 (동아대학교 의과학연구원, 동아대학교병원 임상시험연구센터) ;
  • 강은영 (동아대학교 의과학연구원, 동아대학교병원 임상시험연구센터) ;
  • 최영 (동아대학교 의과학연구원, 동아대학교병원 임상시험연구센터) ;
  • 이재윤 (부산대학교 자연과학대학 미생물학과) ;
  • 천상철 (생물산업연구원) ;
  • 정민호 (동아대학교 의과학연구원, 동아대학교병원 임상시험연구센터) ;
  • 이재동 (부산대학교 자연과학대학 미생물학과)
  • Published : 2006.06.30
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Abstract

Entomopathogenic fungus Cordyceps militaris is famous for its medicinal efficacies. It has been reported to have various pharmacological activities such as anti-tumour, insecticidal, antibacterial, immunomodulatory and antioxidant. In this study, we investigated the effect of the extract of C. militaris (MPUN8501), which was identified by the analysis of the nucleotide sequences of 5.8S ribosomal RNA, on the function of liver. C. militaris powder was extracted using hot water extracts method as time, volume and temperature and using method as differential polarity of organic solvent. Each fraction was tested for the improvement of hepatic enzyme alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) activity. The BuOH extracts (CME) had highest activity which was used for the test of toxicity and efficacy of C. militaris. The enhancing effect of CME on the activity of ADH and ALDH was much more than medicine, drink, natural tea etc. Thus CME promoted the resolution of alcohol and acetaldehyde in rats, inducing recovery to normal condition rapidly. Furthermore, oral administration of CME effectively protected the carbon tetrachloride-induced acute hepatic injury as revealed by the hematological parameters (levels of sGOT and sGPT) and histological observation. CME was ascertained to be safe by regulatory toxicity studies of single dose toxicity and genotoxicity. These results suggest that CME would be useful for the maintaining normal hepatic activity as a functional health food.

수집, 동정된 새로운 동충하초의 간에 관한 기전연구 및 기능향상을 확인하고자 본 연구를 수행하였다. 번데기 동충하초를 버섯채집회 등을 통해서 수집하고, 염기서열 분석을 실시하였다. 분석된 염기서열을 유전자은행에 등록된 번데기 동충하초와 계통발생학적인 비교를 통해 동일종으로 확인하였다. 추출조건 및 방법을 다양화시켜 열수 및 유기용매 추출법으로부터 얻어진 분획중에서 간관련 효소활성 시험을 통해 효능이 있는 추출물 분획을 찾아내고, 이 추출물을 이용하여 간의 대사효소인 ADH, ALDH의 활성증가가 시판되고 있는 의약품, 천연차, 혼합음료, 드링크 등의 6종류의 제품과 비교 실험하여 $500\;{\mu}g/m{\ell}$의 동충하초 추출물이 월등한 활성증가를 확인하였다. 또한 생체에 투여하여 동충하초 추출물에 의해 혈중 알코올과 아세트알데히드의 분해 및 대사가 촉진되고 정상상태로 빠르게 회복됨을 관찰하였다. 그리고 설치류 단회독성시험 및 유전독성시험을 통하여 시험물질의 무독성을 확인하였고, 간을 손상물질인 사염화탄소를 투여하여 급성손상에 대한 혈액생화학변화(GOT, GPT) 및 조직에서 동충하초 추출물에 의한 보호 및 회복작용을 확인하였다. 본 연구에서는 번데기 동충하초의 추출물이 독성물질에 대한 급성적인 반응을 보였지만, 저독성의 오랜기간 노출로 인한 만성간장해와 이로 인해 발생하는 지방간 등에 대한 효과에 대해서는 앞으로 추가적인 연구가 필요할 것으로 사료된다.

Keywords

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