Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Antifungal Activity of Phenanthrene Derivatives from Aerial Bulbils of Dioscorea batatas Decne

재배마 (Dioscorea batatas Decne)의 주아로부터 분리된 phenanthrene 유도체의 항진균 활성

  • Kum, Eun-Joo (Department of Food and Nutrition, Andong National University) ;
  • Park, Sang-Jo (Experiment Station of Bioresources, Gyeongbuk Agricultural Technology Administration) ;
  • Lee, Bong-Ho (Experiment Station of Bioresources, Gyeongbuk Agricultural Technology Administration) ;
  • Kim, Jong-Sik (Department of Biological Science, Andong National University) ;
  • Son, Kun-Ho (Department of Food and Nutrition, Andong National University) ;
  • Sohn, Ho-Yong (Department of Food and Nutrition, Andong National University)
  • 금은주 (안동대학교 식품영양학과) ;
  • 박상조 (안동대학교 생명과학과) ;
  • 이봉호 (안동대학교 생명과학과) ;
  • 김종식 (경북농업기술원 생물자원연구소) ;
  • 손건호 (안동대학교 식품영양학과) ;
  • 손호용 (안동대학교 식품영양학과)
  • Published : 2006.07.31
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Abstract

Plants of the genus Dioscorea have long been used as oriental folk medicine, and Dioscorea batatas Decne has been cultivated for healthy food in Korea. Although the bulbils were produced 2,000 ton annually, there are few reports for bioactive compounds in bulbils. In this study, three phenanthrenes and two phenanthraquinones were isolated from the aerial bulbils of D. batatas Decne, and their structures were elucidated. Among them, compound 2 (6-hydroxy-2,7-dimethoxy-1,4-phenanthraquinone) has not been reported previously. Evaluation of antimicrobial activities based on disk-diffusion assay, MIC and MFC showed the compound 12 (6,7-dihydroxy-2,4-dimethoxyphenanthrene) has strong antimicrobial activity with $25\;{\mu}g/ml$ of MIC and MFC against Candida albicans. Our results suggested that compound 12 has a potent antifungal activity, and the antimicrobial activity and its spectrum are modulated by hydroxylation and methoxylation of phenanthrene ring moiety of the compound.

마속 식물들은 오래 전부터 다양한 민간처방에 사용되어 왔으며, 재배마의 근경은 다양한 생리활성물질들이 보고되어, 건강식품으로 인식되고 있다. 재배마의 주아는 매년 2,000톤 이상 생산되고 있으나, 다음해의 종자로 일부 이용될 뿐 거의 폐기되고 있으며, 생리활성에 대한 보고도 거의 없는 실정이다. 본 연구에서는 마의 주아로부터 3종의 phenanthrene 및 2종의 phenanthraquinone를 분리하였으며, 이들의 구조를 규명하였다. 이들 중 6-hydroxy-2,7-dimethoxy-1,4-phenanthraquinone은 처음으로 분리 되었다. 분리된 5종을 대상으로 항균활성을 평가한 결과, 6,7-dihydroxy-2,4-dimethoxyphenanthrene은 Candida albicans에 대해 $25\;{\mu}g/ml$의 MIC와 MFC를 나타내어, 강력한 항균활성으로 실제적 사용 가능성을 나타내었다. 본 연구결과는 phenanthrene 링 구조의 -OH 및 $-OCH_3$ 수식이 항균활성 및 항균 스펙트럼 결정의 중요한 인자임을 제시하였다.

Keywords

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