Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Protective Activity against Ionizing Radiation of Antioxidative Plants Indigenous to Korea

  • Jung, Myung-Sun (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • Kang, Kyoung-Ah (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • Zhang, Rui (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • Chae, Sung-Wook (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • Yoo, Byoung-Sam (Cosmetic R&D Center, COSMAX Inc.) ;
  • Yang, Young-Taek (Jeju-do Agricultural Research & Extension Services) ;
  • Lee, Nam-Ho (Department of Chemistry, College of Natural Sciences, Cheju National University) ;
  • Park, Jae-Woo (Department of Nuclear and Energy Engineering, Cheju National University) ;
  • Hyun, Jin-Won (Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University)
  • Published : 2006.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

We have screened the cytoprotective effect on ${\gamma}-ray$ radiation induced oxidative stress from forty one Korean plant extracts. Carpinus laxiflora (caulis), Quercus salicina (caulis), and Castanopsis cuspidata (caulis) were found to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and intracellular reactive oxygen species (ROS). As a result, extracts of three plants reduced cell death of Chinese hamster lung fibroblast (V79-4) cells induced by $H_2O_2$ treatment. In addition, these extracts protected cell death of V79-4 cells damaged by ${\gamma}-ray$ radiation. In addition, these extracts scavenged ROS generated by radiation. Taken together, the results suggest that Carpinus laxiflora, Quercus salicina, and Castanopsis cuspidata protect V79-4 cells against oxidative damage by radiation through scavenging ROS.

Keywords

References

  1. Agarwal, O.P. and Nagaratnam, A., Radioprotective property of flavonoids in mice. Toxicon. 19, 201-204 (1981) https://doi.org/10.1016/0041-0101(81)90022-2
  2. Carmichael, J., DeGraff, W.G., Gazdar, A.F., Minna, J.D., and Mitchell, J.B., Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47, 936-941 (1987)
  3. Chang, C.S., and Jeon, J.I., Foliar flavonoids of the most primitive group, sect. Distegocarpus within the genus Carpinus. Biochem. Syst. Ecol. 32, 35-44 (2004) https://doi.org/10.1016/S0305-1978(03)00186-8
  4. Chen, H.F., Tanaka, T., Nonaka, G.I., Fujioka, T., and Mihashi, K., Phenylpropanoid-substituted catechins from Castano psis hystrix and structure revisions of cinchonains. Phytochemistry 33, 183-187 (1993) https://doi.org/10.1016/0031-9422(93)85419-R
  5. Gerschman, R., Gilbert, D.L., Nye, S.W., Dwyer, P., and Fenn, W.O., Oxygen poisoning and x-irradiation: a mechanism in common. Science 119, 623-626 (1954) https://doi.org/10.1126/science.119.3097.623
  6. Gupta, D., Arora, R., Garg, A.P., Bala, M., and Goel, H.G., Modification of radiation damage to mitochondrial system in vivo by Podophyllum hexandrum: Mechanistic aspects. Mol. Cell. Biochem. 266, 65-77 (2004) https://doi.org/10.1023/B:MCBI.0000049139.05337.40
  7. Lee, P.W., Kim, H.S., and Eom, Y.G., Wood anatomy of Genus Carpinus grown in Korea. Seoul Natl. Univ. J. Agric. Sci. 14, 41-48 (1989)
  8. Lo, S.F., Nalawade, S.M., Mulabagal, Y., Matthew, S., Chen, C.L., Kuo, C.L., and Tsay, H.S., In vitro propagation by asymbiotic seed germination and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity studies of tissue culture raised plants of three medicinally important species of Dendrobium. Biol. Pharm. Bull. 27, 731-735 (2004) https://doi.org/10.1248/bpb.27.731
  9. Maurya, D.K., Salvi, V.P., and Krishnan Nair, C.K., Radioprotection of normal tissues in tumor-bearing mice by troxerutin. J. Radiat. Res. 45, 221-228 (2004) https://doi.org/10.1269/jrr.45.221
  10. McClain, D.E., Kalinich, J.F., and Ramakrishnan, N., Trolox inhibits apoptosis in irradiated MOLT-4 lymphocytes. FASEB J. 9, 1345-1354 (1995) https://doi.org/10.1096/fasebj.9.13.7557025
  11. Meng, Z., Zhou, Y, Lu, J., Sugahara, K., Xu, S., and Kodama, H., Effect of five flavonoid compounds isolated from Quercus dentata Thunb on superoxide generation in human neutrophils and phosphorylation of neutrophil proteins. Clin. Chim. Acta. 306, 97-102 (2001) https://doi.org/10.1016/S0009-8981(01)00403-X
  12. Murray, J.I., Whitfield, M.L., Trinklein, N.D., Myers, R.M., Brown, P.O., and Botstein, D., Diverse and specific gene expression responses to stresses in cultured human cells. Mol. Biol. Cell 15, 2361-2374 (2004) https://doi.org/10.1091/mbc.E03-11-0799
  13. Pryor, W.A, Stone, K., Zang, L.Y., and Bermudez, E., Fractionation of aqueous cigarette tar extracts: fractions that contain the tar radical cause DNA damage. Chem. Res. Toxicol. 11, 441-448 (1998) https://doi.org/10.1021/tx970159y
  14. Rithidech, K.N., Tungjai, M., and Whorton, E.B., Protective effect of apigenin on radiation-induced chromosomal damage in human lymphocytes. Mutat. Res. 585, 96-104 (2005) https://doi.org/10.1016/j.mrgentox.2005.04.003
  15. Rosenkranz, A.R., Schmaldienst, S., Stuhlmeier, K.M., Chen, W., Knapp, W., and Zlabinger, G.J., A microplate assay for the detection of oxidative products using 2',7'-dichlorofluorescein-diacetate. J. Immunol. Meth. 156, 39-45 (1992) https://doi.org/10.1016/0022-1759(92)90008-H
  16. Salganik, R.I., The benefits and hazards of antioxidants: controlling apoptosis and other protective mechanisms in cancer patients and the human population. J. Am. Call. Nutr. 20, 464S-472S (2001) https://doi.org/10.1080/07315724.2001.10719185
  17. Shimoi, K., Masuda, S., Shen, B., Furugori, M., and Kinae, N., Radioprotective effects of anti oxidative plant flavonoids in mice. Mutat. Res. 350, 153-161 (1996) https://doi.org/10.1016/0027-5107(95)00116-6
  18. Sokmen, M., Angelova, M., Krumova, E., Pashova, S., Ivancheva, S., Sokmen, A., and Serkedjieva, J., In vitro antioxidant activity of polyphenol extracts with antiviral properties from Geranium sanguineum L. Life Sci. 76, 2981-2993 (2005) https://doi.org/10.1016/j.lfs.2004.11.020
  19. Vivas, N., Nonier, M.F., Gaulejac, N.V., and Boissel, I.P., Occurrence and partial characterization of polymeric ellagitannins in Quercus petraea Liebl. and Q. robur L. Chim. 7, 945-954 (2004) https://doi.org/10.1016/j.crci.2004.06.004
  20. Weiss, J.F., and Landauer, M,R., Radioprotection by antioxidants. Ann. N. Y. Acad. Sci. 899, 44-60 (2000) https://doi.org/10.1111/j.1749-6632.2000.tb06175.x
  21. Weiss, J.F., and Simic, M.G., Perspectives in radioprotection. Pharmacol. Ther. 39, 1-14 (1988) https://doi.org/10.1016/0163-7258(88)90031-9