Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Inhibitory Effect of KIOM-79, a New Herbal Prescription, on AGEs Formation and Expressions of Type IV Collagen and $TGF-{\beta}1$ in STZ-induced Diabetic Rats

복합한약제제 KIOM-79으I STZ 유도 당뇨 모델에서의 최종당화산물 생성 및 Type IV Collagen 및 $TGF-{\beta}1$ 발현 억제 효과

  • Kim, Young-Sook (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Lee, Yun-Mee (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Kim, Chan-Sik (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Sohn, Eun-Jin (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Jang, Dae-Sik (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine) ;
  • Kim, Jin-Sook (Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine)
  • 김영숙 (한국한의학연구원 한약제제연구부) ;
  • 이윤미 (한국한의학연구원 한약제제연구부) ;
  • 김찬식 (한국한의학연구원 한약제제연구부) ;
  • 손은진 (한국한의학연구원 한약제제연구부) ;
  • 장대식 (한국한의학연구원 한약제제연구부) ;
  • 김진숙 (한국한의학연구원 한약제제연구부)
  • Published : 2006.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Advanced glycation end products (AGEs) formation plays an important role in the progression of diabetic complications. To develop effective herbal formulations with suppression of diabetic nephropathy, a common complication in diabetic patients, we evaluated inhibitory activities of KIOM-79, a new herbal prescription, on the formation of AGEs using in vitro and in vivo model systems. Effects of KIOM-79 on the expression of AGEs, RAGEs (receptor for Advanced glycation end products), type IV collagen and renal $TGF-{\beta}1$ mRNA was also examined in streptozotocon (STZ)-induced diabetic rats. STZ-induced diabetic rats were treated orally with KIOM-79 (250 and $500\;kg^{-1}$ once a day for 13 weeks). In vitro system KIOM-79 suppressed the formation of AGEs $(18.12\;{\mu}g/ml)$. In STZ-induced diabetic rats showing accumulation of AGE and RAGE, pathological examination revealed that KIOM-79 prevented AGE and RAGE deposition in the kidney. In STZ induced diabetic rats, the expansion of mesangial matrix and the glomerular tufts seemed to be larger than those in normal rats. Howεver, after administration with KIOM-79, mesangial metrix and glomerular volume were decreased, and overexpression of type IV collagen was also decreased. Overexpression of renal $TGF-{\beta}1$ mRNA was inhibited significantly. These results suggest that the KIOM-79 might be an effective herbal prescription to prevent or alleviate the progression of diabetic nephropathy.

Keywords

References

  1. 대역동의보감 (對譯東醫寶鑑) (1999) 1337. 법인문화사, 서울
  2. 배병철 (1999) 금석 황제 내경 소문, 제 37, 370, 성보사, 서울
  3. LeRoith, D. and Taylor, S.I. (2003) Diabetes mellitus a Fundamental and clinical text. In Hofinann, S. and Brownlee, M.(ed.), Biochemistry and molecular cell biology of Diabetic Complications A Unifying Mechanism, 1444, Lippincott Williams & Wilkins, Philadelphia
  4. Edelstein, D. and Brownlee, M. (1992) Mechanistic studies of advanced glycosylation end product inhibition by aminoguanidine. Diabetes 41: 26-29 https://doi.org/10.2337/diabetes.41.1.26
  5. Kowluru, R.A. (2005) Effect of advanced glycation end products on accelerated apoptosis of retinal capillary cells under in vitro condition. Life Science 76: 1051-1060 https://doi.org/10.1016/j.lfs.2004.10.017
  6. Jang, D.S., Lee, Y.M., Kim, Y.S. and Kim, J.S. (2006) Screening of Korean Traditional Herbal Medicines with inhibitory activity on Advanced Glycation End Products (AGEs) Formation. Kor. J. Pharmacogn. 37: 48-52
  7. 김호철 (2001) 한약약리학, 434. 집문당, 서울
  8. Jeon, Y.J., Li, M.H., Lee, K.Y., Kim, J.S., You, H.J., Lee, S.K., Sohn, H.M., Choi, S.J., Koh, J.W. and Chang, I.Y. (2006) KIOM-79 inhibits LPS-induced iNOS gene expression by blocking NF-kB/Rel and p38 kinase in murine macrophages. J. of Ethnopharmacology, accepted
  9. Kim, J.S., Kim, H.Y. and Ko, J.H. (2002) Studies om the processing of herbal medicines (III)-HPLC Analysis of Magnolol and Inhibitory Effects on the Formation of Advanced Glycation Endproducts(AGEs) in vitro of Unprocessed and Processed Magnoliae Bark-. Kor. J. Pharmacogn. 33: 308-311
  10. Lee, J.Y and Kang, B.S. (1994) Processing of herb medicines and clinical application, 124. Younglim publishes Co., Seoul
  11. Lee, J.Y. and Kang, B.S. (1994) Processing ofherb medicines and clinical application, 136. Younglim publishes Co., Seoul. 109
  12. Abiko, T.A., Abiko, S., Ishiko, M., Takeda, M., Horiuchi, S. and Yoshida, A. (1999) A Relationship between autofluorescence and advanced glycation end products in diabetic lenses. Exp. Eye Res. 68: 361-366 https://doi.org/10.1006/exer.1998.0615
  13. Park, Y., Lee, H., Koh, C.S., Min, H., Yoo, K., Kim, Y. and Shin, Y. (1995) Prevalence of diabetes and lar in Yonchon county, South Korea. Diabetes care 18: 545-548 https://doi.org/10.2337/diacare.18.4.545
  14. 검호철 (2001) 한약약리학, 92. 집문당, 서울
  15. Kang, K.A., Chae, S., Koh, Y.S., Kim, J.S., Lee, J.H., You, H.J. and Hyun, J.W. (2005) Protective effect of puerariae radix on oxidative stress induced by hydrogen peroxide and streptozotocin. Biol. Pharm. Bull. 28: 1154-1160 https://doi.org/10.1248/bpb.28.1154
  16. Chen, W.C., Hayakawa, S., Yamamoto, T., Su, H.C. and Cheng, I.M. (2004) Mediation of beta-endorphin by the isoflavone puerarin to lower plasma glucose in streptozotocin- induced diabetic rats. Planta Med. 70: 113-116 https://doi.org/10.1055/s-2004-815486
  17. 김호철 (2001) 한약약리학, 211. 집문당, 서울
  18. 김호철 (2001) 한약약리학, 181. 집문당, 서울
  19. Alarcon-Aguilara, FJ., Roman-Ramos, R., Perez-Gutierrez, Aguilar-Contreras, A., Contreras-Weber, CC. and FloresSaenz, JL. (1998) Study of anti-hyperglycemic effect of plants used as antidiabetics. J. of Ethnopharmacology 61: 101-110 https://doi.org/10.1016/S0378-8741(98)00020-8
  20. Chizuko, H., Kanji, Y. and Toshihide, Y. (2004) Efficacy of bofu-tsusho-san, an oriental herbal medicine, in obese Japanese women with impaired glucose tolerance. Clin. Exp. Pharmacol. Physiol. 31: 614-619 https://doi.org/10.1111/j.1440-1681.2004.04056.x
  21. Aida, K., Tawata, M., Shindo, H., Onaya, T., Sasaki, T., Yamaguchi, T., Chin, T. and Mitsuhashi, H. (1990) Isoliquiritigenin: a new aldose reductase inhibitor from glycyrrhizae radix. Planta Med. 56: 254-258 https://doi.org/10.1055/s-2006-960950
  22. 김호철 (2001) 한약약리학, 434 : 집문당, 서울
  23. Cooper, M.E. (2000) Interaction of metabolic and haemodynamic factors in mediating experimental diabetic nephropathy. Diabetologia 44: 1957-1972 https://doi.org/10.1007/s001250100000
  24. Rumble, J.R., Cooper, M.E., Soulis, Cox, T., Wu, A.L. and Youssef, S., Jasik, M., Jerums, G. and Gibert, R.E. (1997) Vascular hypertrophy in experimental diabetes. J. Clin. Invest. 99: 1016-1027 https://doi.org/10.1172/JCI119229
  25. Sharma, K. and Ziyadeh, F.N. (1995) Hyperglycemia and diabetic kidney disease. The case for transforming growth factor- beta as a key mediato. Diabetes 44: 1139-1146 https://doi.org/10.2337/diabetes.44.10.1139
  26. Nakamura, N., Yamazaki, K., Satoh, A., Urakaza, M., Kobayashi, M., Yamabe,H., Osawa, H., Shirato, K., Sugawara, T., Nakamura, M., Tamura, M. and Okumara, K. (2003) Effects of epalrestat on plasma levels of advanced glycation end products in patients with type 2 diabetes. In Vivo 17: 177-180
  27. Ishii, H., Tada, H. and Isogai, S. (1998) An aldose reductase inhibitor prevents glucose-induced increase in transforming growth factor-beta and protein kinase C activity in cultured mesangial cells. Diabetologia 41: 362-364 https://doi.org/10.1007/s001250050916