Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Effects of Alcohol Oxidation of Brassica rapa L. Extraction Process in Kang-Hwa

강화산 순무의 추출 공정에 따른 알코올 분해 효과

  • Kim, Dae-Ho (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Kim, Jung-Hwa (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Kim, Cheol-Hee (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Kwon, Min-Chul (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Kim, Hyo-Sung (School of Biotechnology and Bioengineering, Kangwon National University) ;
  • Chung, Hae-Gon (Ganghwa Agricultural R&D Center) ;
  • Kang, Ha-Young (Forest Research Institute) ;
  • Lee, Hak-Ju (Forest Research Institute) ;
  • Lee, Hyeon-Yong (School of Biotechnology and Bioengineering, Kangwon National University)
  • 김대호 (강원대학교 바이오산업공학부) ;
  • 김정화 (강원대학교 바이오산업공학부) ;
  • 김철희 (강원대학교 바이오산업공학부) ;
  • 권민철 (강원대학교 바이오산업공학부) ;
  • 김효성 (강원대학교 바이오산업공학부) ;
  • 정해곤 (강화농업기술센타) ;
  • 강하영 (삼림과학연구소) ;
  • 이학주 (삼림과학연구소) ;
  • 이현용 (강원대학교 바이오산업공학부)
  • Published : 2006.02.28
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Abstract

Alcohol dehydrogenase (ADH) and Aldehyde dehydroganase (ALDH) activities of turnip extracts with ultrasonification at $60,\;100^{\circ}C$ temperature were investigated. Ultrasonification extracts showed a increase as compared with the normal extracts. ALDH activities were high than ADH activities. So, turnip extracts was shown efficient plants in alcohol oxidation. Appraisers liked powder extracts better than liquid extracts in sensory score.

순무는 초음파 병행 추출을 통하여 $60,\;100^{\circ}C$에서 모두 추출 수율을 증가시킬 수 있음을 확인하였다. 알코올 분해 효소인 ADH와 Aldehyde 분해 효소인 ALDH의 분해 활성 측정 실험을 통해 순무의 추출물들은 물 일반 추출물보다 초음파 병해 추출물들이 높은 활성을 나타내는 것을 확인하였고 특히 ADH의 활성율 보다 ALDH의 활성이 높게 나타나 알코을 분해 과정의 최종 목적지인 acetic acid와 이산화탄소로의 분해에 순무의 추출물들이 유의적인 활성을 나타내고 있음을 확인할 수 있었다. $60^{\circ}C$ 열수 추출물은 $100^{\circ}C$ 열수 추출물과 비교하여 관능평가 시 높은 점수를 얻었으며 이의 추출물들은 감압농축을 통해 이취의 제거가 이루어져 열수추출 시 유용한 방법으로 평가되었다.

Keywords

References

  1. Choi JY, Joo CN (1993) Probable Reaction Mechanism of Rat Liver Cytosolic ALDH. Korean Biochem. J. 26:26-33
  2. Collins J (1978) Flavor preference of selected food products from vegetables. J. Agric. Food chem. 26:1012-1015 https://doi.org/10.1021/jf60219a040
  3. Hertog MGL, Hollman PCH, Katan MB (1992) Content of potentially anti carcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands. J. Agric. Food Chem. 40:2379-2383 https://doi.org/10.1021/jf00024a011
  4. Itoh H, Yoshida R, Mizuno T, Kudo M, Nikuni S, Karki T (1984) Study on the contents of volatile isothiocyanate of cultivars of Brassica vegetables. Report of the National Food Research Institute 45:33-41
  5. Ju H, Chong C, Mullin W, Bilble B (1982) Volatile isothiocyanates and nitriles from glucosinolates in rutabaga and turnip. J. Am. Soc. Horicultural Sci. 107:1050-1054
  6. Kang BK, Jung ST, Kim SJ (2002) Effects of Vegetable Extracts by Solvent Separation on Alcohol Dehydrogenase Activity from Saccharomyces cerevisiae. Korean J. Food Sci. Technol. 34:244-248
  7. Kang IH (1991) History of Culture in Korea (II). Samyoungsa. Seoul. Korea. p. 197
  8. Kawabata A, Sawayama S (1973) A study on the content of pectic substances in vegetables. J. Japan Nutr. 31:32-36 https://doi.org/10.5264/eiyogakuzashi.31.32
  9. Kim BH, Zeikus JG (1992) Specificity of alcohol dehydrogenase from Clostridium acetobutylicum ATCC4259. J. Microbiol. Biotechnol. 4:268-272
  10. Miyao S, Aoki M (1979) Quality and behaviour of microorganisms in pickles. II. Lactic acid fermentation of turnips. J. Japan. Food Sci. Tech. 26:444-446 https://doi.org/10.3136/nskkk1962.26.10_444
  11. Mongeau R, Brassard R (1993) Enzymatic-gravimetric determination in foods of dietary fiber as sum of insoluble and soluble fiber fractions: summary of collaborative study. J. Assoc. Off. Anal. Chem. 76:923-925
  12. Morita M, Miyamoto T, Mori K, Kataoka K, Izumimoto M (1990) Isolation and identification of lactic acid bacteria from pickles. J. Japan Dairy and Food Sci. 39:A183-A193
  13. Oh SH, Oh YK, Park HH, Kim MR (2003) Physicochemical and Sensory Characteristics of Turnip Pickle Prepared with Different Pickling Spices During Storage. Korean Journal of Food Preservation 10(3):347-353
  14. Pares X, Farres XPJ, Moreno A, Saubi N, Boleda MD, Cederlund E, Hoog JO, Jornvall H (1993) Class alcohol dehydrogenase: structure and function. In: Enzymology and Molecular Biology of Carbonyl Metabolism 4. Plenum Press. New York. USA. p. 475-481
  15. Park YK, Kim HM, Park MW, Kim SR, Choi IW (1999) Physicochemical and functional properties of turnip. J. Korean Soc. Food Sci. Nutr. 28:333-341
  16. Sones K, Heaney R, Fenwick G (1984) An estimate of the mean daily intake of glucosinolates from cruciferous vegetables in the UK. J. Sci. Food Agric. 35:712-720 https://doi.org/10.1002/jsfa.2740350619
  17. Wilkinson A, Rhodes M, Fenwick R (1984) Myrosinase activity of cruciferous vegetables. J. Sci. Food Agric. 35:543-552 https://doi.org/10.1002/jsfa.2740350511
  18. Yamani M (1993) Fermentation of brined turnip roots using Lactobacillus plantarum and Leuconostoc mesenteroides starter cultures. World J. Micro. and Biotech. 9: 176-179 https://doi.org/10.1007/BF00327831
  19. 육창수 (1981) 한국약품식물자원도감. 진명출판사 p. 120