Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Development of Biologically Active Compounds from Edible Plant Sources-XVII. Isolation of Sterols from the Fruits of Cornus kousa Burg

식용식물자원으로부터 활성물질의 탐색-XVII. 산딸나무(Cornus kousa Burg.)의 열매로부터 sterol 화합물의 분리

  • Lee, Dae-Young (The Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Song, Myoung-Chong (The Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Yoo, Jong-Su (The Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Kim, Sung-Hoon (Graduate School of Oriental Medicine, Kyung Hee University) ;
  • Chung, In-Sik (The Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Kim, Dae-Keun (Department of Pharmacy, Woosuk University) ;
  • Park, Mi-Hyun (Erom life Co. Ltd.) ;
  • Kwon, Byoung-Mog (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Se-Young (The Graduate School of Biotechnology & Plant Metabolism Research Center) ;
  • Baek, Nam-In (The Graduate School of Biotechnology & Plant Metabolism Research Center)
  • 이대영 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 송명종 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 유종수 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 김성훈 (경희대학교 한의과대학원) ;
  • 정인식 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 김대근 (우석대학교 약학대학) ;
  • 박미현 ((주)이롬라이프) ;
  • 권병목 (한국생명공학연구원) ;
  • 김세영 (경희대학교 생명공학원 및 식물대사연구센터) ;
  • 백남인 (경희대학교 생명공학원 및 식물대사연구센터)
  • Published : 2006.03.31
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Abstract

The fruits of Cornus kousa Burg. were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc fraction, three sterols were isolated through the repeated silica gel and ODS column chromatographies. According to the results of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as ${\beta}-sitosterol$ (1), $stigmast-4-en-6{\beta}-ol-3-one$ (2) and daucosterol (3). They were the first to be isolated from Cornus kousa Burg.

산딸나무 열매를 100% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매 분획 하였다. 이 중 EtOAc 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography를 반복하여 3종의sterol 화합물을 분리, 정제하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, ${\beta}-sitosterol$(1), $stigmast-4-en-6{\beta}-ol-3-one$(2), daucosterol(3)으로 동정하였다. 이 화합물들은 산딸나무에서 처음 분리되었다.

Keywords

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