Applied Biological Chemistry

  • 제49권3호
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  • Pages.248-253
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  • 2006
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  • 2468-0834(pISSN)
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  • 2468-0842(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지

조각인(Gleditsiae Semen) 추출물의 대장암 세포주에 대한 세포독성효과

The Cytotoxic Effect of the Gleditsiae Semen Extracts on Human Colon Carcinoma Cells

  • 차미란 (경남대학교 식품생명학과) ;
  • 윤미영 (경남대학교 식품생명학과) ;
  • 김주영 (경남대학교 식품생명학과) ;
  • 황지환 (경남대학교 식품생명학과) ;
  • 박해룡 (경남대학교 식품생명학과)
  • Cha, Mi-Ran (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Yoon, Mi-Young (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Kim, Ju-Young (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Hwang, Ji-Hwan (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Park, Hae-Ryong (Department of Food Science and Biotechnology, Kyungnam University)
  • 발행 : 2006.09.30
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초록

조각인(Gleditsiae Semen)은 동아시아 지역에서 장 기능을 향상시키는 등의 여러 가지 민간요법으로 사용되는 한약재이다. 본 연구에서는 조각인의 항암활성을 검색하기 위한 목적으로 methanol, ethanol, acetone으로 추출한 뒤, 인간 유래의 대장암 세포주 HT-29에 처리하여 각 추출물의 암세포 성장 억제능을 확인하기 위하여 MTT-dye reduction assay를 이용하여 실험하였다. 그 결과, methanol 추출물에서 가장 높은 세포독성을 확인할 수 있었고, ethanol 추출물에서도 비교적 높은 암세포 성장 억제능을 볼 수 있었으나, acetone 추출물에서는 거의 세포독성이 나타나지 않았다. 그리고 추출물들을 $25\;{\mu}g/ml$의 농도로 암세포에 처리하여 위상차 현미경으로 세포주의 형태학적 변화를 관찰한 결과에서도 methanol 추출물에서의 활성이 가장 강력하게 나타남을 알 수 있었다. 조각인의 항암활성물질들의 용매특성을 알아보기 위하여 methanol 추출물을 극성도에 따라 용매분획 하여 분리한 각 분획물의 세포독성을 확인한 결과, water층 분획물에서 농도 의존적으로 세포독성을 나타냄을 확인하였다. 또한 조각인의 water층 분획물은 또 다른 대장암 세포주인 SW620에 대해서도 대조군에 비해 $25\;{\mu}g/ml$의 농도에서 2배 정도 높은 항암활성을 나타내고 있음을 MTT reduction assay와 LDH release assay를 통하여 확인 할 수 있었다.

The present study describes the preliminary evaluation of the cytotoxicity from Gleditsiae Semen extracts. G. Semen was extracted with methanol, ethanol, and acetone, and then cytotoxic effect of these extracts was measured by the MTT reduction assay and phase-contrast microscopy on the HT-29 human colon carcinoma cells. Among these extracts, methanol extract showed the highest cytotoxic activity on the HT-29 cells. The methanol extract was further fractionated with n-hexane, diethyl ether, ethyl acetate, and water layer according to the degree of polarity. The water layer showed the highest inhibitory activity on the growth of HT-29 cells, but the other fractions indicated the low cytotoxic activity. In addition, water layer also showed the cytotoxic activity against SW620 human colon carcinoma cells. Based on these results, we suggest that extracts of G. Semen may contain bioactive materials and are potential candidates as chemotherapeutic agents against human colon carcinoma cells.

키워드

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