Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Oxidative Stress of Mouse Fed Irradiated Diet Containing High Unsaturated Fatty Acid

방사선 조사된 고불포화지방산 식이를 섭취한 Mouse의 산화적 스트레스

  • Seo, Dae-Young (Department of Food and Nutrition, Chungnam National University) ;
  • Park, Sun-Young (Department of Food and Nutrition, Chungnam National University) ;
  • Kang, Myoung-Hee (Department of Food and Nutrition, Hannam University) ;
  • Suh, Kwang-Sun (Department of Pathology, Chungnam National University School of Medicine) ;
  • Ly, Sun-Yung (Department of Food and Nutrition, Chungnam National University)
  • 서대영 (충남대학교 생활과학대학 식품영양학과) ;
  • 박선영 (충남대학교 생활과학대학 식품영양학과) ;
  • 강명희 (한남대학교 식품영양학과) ;
  • 서광선 (충남대학교 의과대학 병리학교실) ;
  • 이선영 (충남대학교 생활과학대학 식품영양학과)
  • Published : 2006.10.31
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Abstract

In this study, we investigated the in vivo effect by intake of the irradiated foods such as mackerel and sesame seed which are high in unsaturated fatty acid through TBARS (thiobarbituric acid reactive subtance) and the tissue pathological and genotoxicological test. Thirty two ICR mice are divided into four groups, one non-irradiated (control) group and three irradiated (5, 10, 20 kGy, respectively) groups. Sesame seed and pulverized mackerel in modified AIN93M diet were mixed together then divided into four identical parts. Three parts of them were irradiated by doses of 5, 10, and 20 kGy. These experimental diet were fed to each group for 4, 8 and] 6 weeks. The results of the study were as follows: No significant differences in weight gain were found in each group. Peroxide value of the irradiated diet was higher than that of the non-radiated one and also increased according to the storage period. TBA values in plasma, liver, kidney and Peyer's patch were not significantly different among 4 groups. DNA% in tail, tail length (TL) and tail moment (TM) values of the blood lymphocyte in 4, 8 and 16 week groups and the liver in 16 week group were much measured over the control DNA % in tail of kidney of 8 week group was significantly than the control and TL and TM of 8 week and TM of 16 week groups showed a tendency of higher values. By Peyer's DNA % in tail of 8 week group, DNA % in tail and TM of 16 week groups increased significantly over the control, Ultrastructural examination shows myeline figures and swollen mitochondria in parietal cells and intestinal epithelial cells of 8 and 16 weeks groups. After this study, we need further investigations on the safety of highly consumed foods which contain high contents of unsaturated fatty acid, largely imported and which are possible to be irradiated.

Keywords

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