Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Novel Suspension-Phase Enzyme Reaction System Using Insoluble Extrusion Starch as Glycosyl Donor for Intermolecular Transglycosylation of L-Ascorbic Acid

  • Kim, Tae-Kwon (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • Jung, Se-Wook (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • Go, Young-Hoon (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • Lee, Yong-Hyun (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University)
  • Published : 2006.11.30
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Abstract

A novel suspension-phase enzyme reaction system for the intermolecular transglycosylation of L-ascorbic acid into 2-O-${\alpha}$-D-glucopyranosyl L-ascorbic acid supplementing extrusion starch as the glycosyl donor was developed using cyclodextrin glucanotransferase from Thermoanaerobacter sp. A high conversion yield compared to the conventional soluble-phase enzyme reaction system using cyclodextrins and soluble starch was achieved. The optimal reaction conditions were 2,000 units of cycIodextrin glucanotransferase, 20 g/l of L-ascorbic acid, and 50 g/l of extrusion starch at $50^{\circ}C$ for 24 h. The new suspension-phase enzyme reaction system also exhibited several distinct advantages other than a high conversion yield, including a lower accumulation of oligosaccharides and easily separable residual extrusion starch by centrifugation or filtration in the reaction mixture, which will facilitate the purification of 2-O-${\alpha}$-D-glucopyranosyl L-ascorbic acid. The new suspension-phase enzyme reaction system seems to be potentially applicable as the industrial process for the production of thermally and oxidatively stable 2-O-${\alpha}$-D-glucopyranosyl L-ascorbic acid.

Keywords

References

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