Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Improvement of Selection Efficiency for Bacterial Blight Resistance Using SNP Marker in Rice

SNP 마커를 이용한 벼 흰잎마름병 저항성 선발 효율 증진

  • Shin, Woon-Chul (Rice Breeding & Cultivation Division, Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Baek, So-Hyeon (Rice Breeding & Cultivation Division, Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Seo, Chun-Sun (Rice Breeding & Cultivation Division, Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Kang, Hyeon-Jung (Rice Breeding & Cultivation Division, Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Kim, Chung-Kon (Rice Breeding & Cultivation Division, Honam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Shin, Mun-Sik (Rice Ecology & Breeding Division, Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA) ;
  • Lee, Gang-Seob (Cell & Genetics Division, National Institute of Agricultural Biotechnology, RDA) ;
  • Hahn, Jang-Ho (Cell & Genetics Division, National Institute of Agricultural Biotechnology, RDA) ;
  • Kim, Hyun-Soon (Research & Development Bureau, RDA)
  • 신운철 (호남농업연구소 벼육종재배과) ;
  • 백소현 (호남농업연구소 벼육종재배과) ;
  • 서춘순 (호남농업연구소 벼육종재배과) ;
  • 강현중 (호남농업연구소 벼육종재배과) ;
  • 김정곤 (호남농업연구소 벼육종재배과) ;
  • 신문식 (영남농업연구소 벼생태육종과) ;
  • 이강섭 (농업생명공학연구원 세포유전과) ;
  • 한장호 (농업생명공학연구원 세포유전과) ;
  • 김현순 (농촌진흥청 연구개발국)
  • Published : 2006.12.30
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Abstract

Discovery of single nucleotide polymorphisms (SNPs), including small insertions and deletions, is one of the hot topics in genetic research. The most common type of sequence variant consists of single base differences or small insertions and deletions at specific nucleotide positions. Significance of SNPs in rice is increasing for genetic research, positional cloning and molecular breeding. $F_2$ 170 lines and $F_3$ 194 lines derived from Sangjuchalbyeo/HR13721-53-3-1-3-3-2-2 Were used for Searching SNP markers related to bacterial blight resistance. Sangjuchalbyeo is susceptible to bacterial blight, but HR13721-53-3-1-3-3-2-2 has Xa1 gene resistant to bacterial blight. Individual lines were inoculated with $K_1$ race of bacterial blight and resistant or susceptible was evaluated after 3 weeks from inoculation. The genotypes of population were analysed by PCR-RFLP for SNP marker developing. The segregation of $F_2\;and\;F_3$ population showed almost 3:1, 1:1 ratio, respectively. Analysis of genotype using SNP marker is capable of confirming resistance for $K_1$ race and genotype through amplifying the gene using 16PFXal primer and digested the PCR product with Eco RV. There were close relation between resistance test for $K_1$ race and SNP marker genotype. Especially, DNA analysis using SNP marker is capable of judging homozygote/heterozygote in $F_2$ population compared with resistant test for Kl race. So, it seems to improve the selection efficiency in disease resistant breeding.

본 연구는 흰잎마름병 K1 레이스에 감수성인 상주찰벼와 저항성인 HR13721-53-3-1-3-3-2-2를 인공교배하여 육성된 F2, F3를 재료로 하천 Kl 레이스에 대한 저항성 검정과 SNP 마커를 이용한 유전자형 분석 및 저항성과의 연관성을 분석하였다. Kl 레이스에 대한 저항성 검정 결과 $F_2,\;F_3$에서 각각 이론적 분리비인 3:1, 1:1의 분리비를 나타냈으며 SNP 마커를 이용한 유전자형 분석은 16PFXa1 primer를 이용하여 유전자를 증폭한 후 Eco RV 제한효소 처리하여 다형성을 분석하여 저항성 및 유전자형을 확인할 수 있었다. K1 레이스에 대한 저항성 검정과SNP마커를 이용한 유전자형의 연관분석 결과 저항성과 마커간에 연관성이 일치하였으며, 특히 SNP 마커를 이용한 유전자형 분석에서는 K1 레이스에 대한 저항성 검정에서 알 수 없었던 $F_2$ 개체가 동형접합체인지 이형접합체인지를 판별할 수 있어 저항성 품종 육종을 위한 선발 효율을 높일 수 있었다.

Keywords

References

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