식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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콩(Glycine max)에서 콩위축바이러스(Soybean dwarf virus)의 최초 발생보고

First Report of Soybean Dwarf Virus on Soybean(Glycine max) in Korea

  • 김상목 (농업과학기술원 식물병리과) ;
  • 이재봉 (농업과학기술원 식물병리과) ;
  • 이영훈 (농업과학기술원 식물병리과) ;
  • 최세훈 (농업과학기술원 식물병리과) ;
  • 최홍수 (농업과학기술원 식물병리과) ;
  • 박진우 (농업과학기술원 식물병리과) ;
  • 이준성 (농업과학기술원 식물병리과) ;
  • 이관석 (농업과학기술원 농업해충과) ;
  • 문중경 (작물과학원 작물기능개발과) ;
  • 문제선 (한국생명공학연구원 식물유전체연구센터) ;
  • 이기운 (경북대학교 응용생명과학부) ;
  • 이수헌 (농업과학기술원 식물병리과)
  • Kim, Sang-Mok (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee, Jae-Bong (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee, Yeong-Hoon (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Choi, Se-Hoon (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Choi, Hong-Soo (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Park, Jin-Woo (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee, Jun-Seong (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee, Gwan-Seok (Division of Entomology, National Institute of Agricultural Science and Technology, RDA) ;
  • Moon, Jung-Kyung (Breeding Resource Development Division, National Institute of Crop Science, RDA) ;
  • Moon, Jae-Sun (Plant Genome Research Center, Korea Research Institute of Bioscience & Biotechnology) ;
  • Lee, Key-Woon (School of Applied Biosciences, Kyungpook National University) ;
  • Lee, Su-Heon (Plant Pathology Division, National Institute of Agricultural Science and Technology, RDA)
  • 발행 : 2006.12.01
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초록

2003년 청송지역 농가포장에서 심한 위축증상을 나타내는 콩 시료를 채집하였다. 이 시료를 RT-PCR로 진단한 결과 콩위축바이러스, Soybean dwarf virus(SbDV-L81)에 감염된 것으로 확인되었다. 본 연구는 우리나라에서 콩위축바이러스의 발생에 관한 최초 보고이다. 이 바이러스의 추가적인 특징을 알아보기 위하여 종 특이적 프라이머를 이용한 RT-PCR로 게놈의 부분 염기서열을 결정하였다. 결정된 염기서열은 병징 발현 유형과 매개충 특이성에 기초하여 이전에 조사된 SbDV 계통들과 비교분석하였다. ORF 2와 ORF 3 유전자사이 영역(intergenic region)과 ORF 2, ORF 3 및 ORF 4의 코팅영역에서 SbDV-L81은 황화계통(SbDV-YS and YP) 보다 위축계통(SbDV-DS and DP)과 유사한 특징을 보였다. 한편, ORF 5의 5'쪽 코딩영역은 완두수염진딧물(Acyrthosiphon pisum)이 매개충인 계통(SbDV-YP and DP)과 밀접한 관련이 있은 것으로 나타났다. SbDV-L81은 자연상태에서의 병정과 5가지 영역의 염기서열 비교결과에서 SbDV-DP 계통과 밀접한 관련이 있는 것으로 생각된다.

In year 2003, a soybean(Glycine max) sample showing severe dwarfing symptom was collected from a farmers' field in Cheongsong in Korea. The results from the diagnosis of the sample by RT-PCR revealed that it was infected by Soybean dwarf virus(SbDV), SbDV-L81. This study could be the first report of the occurrence of the virus in Korea. To further characterize the virus, the partial nucleotide sequence of the genomic RNA of SbDV-L81 was determined by RT-PCR using species-specific primers. The sequences were analyzed and subsequently compared to previously characterized strains of SbDV based on the pattern of symptom expression and vector specificities. The intergenic region between ORF 2 and 3 and the coding regions of ORF 2, 3 and 4 were relatively similar to those of dwarfing strains(SbDV-DS and DP) rather than those of yellowing strains(SbDV-YS and YP). Likewise, the result from the analysis of 5'-half of the coding region of ORF5 indicated that SbDV-L81 was closely related to strains(SbDV-YP and DP) transmitted by Acyrthosiphon pisum. These data from the natural symptom and the comparisons of five regions of nucleotide sequences of SbDV suggested that SbDV-L81 might be closely related SbDV-DP.

키워드

참고문헌

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