Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Characteristics of Cucumber mosaic virus-VCH Causing Vein Chlorosis on Red Pepper in Korea

고추에 엽맥퇴록병을 일으키는 오이 모자이크 바이러스(CMV-VCH)의 특징

  • Cho, Jeom-Deog (National Horticultural Research Institute, Rural Development Administration) ;
  • Lee, Sin-Ho (National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Jeong-Soo (National Horticultural Research Institute, Rural Development Administration) ;
  • Choi, Gug-Seoun (National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Hyun-Ran (National Horticultural Research Institute, Rural Development Administration) ;
  • Chung, Bong-Nam (National Horticultural Research Institute, Rural Development Administration) ;
  • Ryu, Ki-Hyun (Seoul Women's University)
  • 조점덕 (농촌진흥청 원예연구소 원예환경과) ;
  • 이신호 (농촌진흥청 원예연구소 원예환경과) ;
  • 김정수 (농촌진흥청 원예연구소 원예환경과) ;
  • 최국선 (농촌진흥청 원예연구소 원예환경과) ;
  • 김현란 (농촌진흥청 원예연구소 원예환경과) ;
  • 정봉남 (농촌진흥청 원예연구소 원예환경과) ;
  • 류기현 (서울여자대학교 환경생명과학대학)
  • Published : 2006.12.01
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Abstract

Cucumber mosaic virus(CMV) was occurred on red pepper showing vein chlorosis or vein necrosis with the incidence rate of 52% from 62 specimens collected in natural fields. Among 32 samples infected with CMV, the specimens of 22 red pepper leaves showing vein chlorosis were infected singly with CMV-VCH. CMV-VCH induced vein chlorosis on the inoculated leaves, and vein banding and vein necrosis on the upper leaves of Nicotiana glutinosa, and then killed after showing bud necrosis. The typical symptoms of vein banding, malformation and blister were produced on the upper leaves of Nicotiana benthamiana and N. tabacum 'Ky-57' without symptoms on the inoculated leaves. The commercial cultivars of 'Bugang', 'Manitta' and 'Gwariput' were shown the typical symptom of vein chlorosis by the mechanical inoculation of CMV-VCH. CMV-VCH was detected specifically by RT-PCR. Virus particles of CMV-VCH were isometric shape having 30 nm diameter. Ultraviolet absorption of purified CMV-VCH was maximum at 260 nm and minimum at 242 nm. The ratio of A260/A280 was 1.71. CMV-VCH had the single nucleo-protein having the molecular weight of 24.5 kDa.

노지 포장에서 채집한 고추 잎에 바이러스 병징을 나타내는 총 62개 시료 중 32개(52%)시료가 CMV에 감염되었다. CMV에 감염된 32개체 중 22개(68.8%) 시료는 엽맥퇴록 및 엽맥괴저 병징을 나타냈으며 모두 CMV(CMV-VCH)에 단독감염되어 있었다. 생물검정결과 Nicotiana glutinosa에서 접종엽에 엽맥퇴록, 상엽에 엽맥녹대, 엽맥괴저, 순괴저에 이르는 심한 병징을 보였고, N. benthamiana와 N. tabacum cv. 'Ky-57'에서 접종엽에 무병징, 상엽에 엽맥녹대, 기형 및 물집 병징을 일으켰다. 고추 시판품종인 '부강', '마니따' 그리고 '꽈리풋고추' 모두 엽맥퇴록 병징이 나타났으며 후기에는 기형이 되었다. RT-PCR 유전자 진단결과 엽맥퇴록 병징의 시료는 모두 CMV에 감염되었으며, CMV-VCH 바이러스 입자는 약 30 nm의 구형 이었다. 자외선 흡광도는 260nm에서 최고, 242nm에서 최저였으며 핵산과 단백질 비율은 1.71이었다. CMV-VCH의 핵 단백질은 단일 단백질이며 분자량은 24.5kDa 이었다.

Keywords

References

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