Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Analysis of a Prodigiosin Biosynthetic Gene Cluster from the Marine Bacterium Hahella chejuensis KCTC 2396

  • Kim, Doc-Kyu (Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Yon-Kyoung (Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Jong-Suk (Korea Research Institute of Bioscience and Biotechnology) ;
  • F. Kim, Ji-Hyun (Korea Research Institute of Bioscience and Biotechnology) ;
  • Jeong, Hae-Young (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Beom-Seok (College of Life and Environmental Sciences, Korea University) ;
  • Lee, Choong-Hwan (Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2006.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Marine bacterium Hahella chejuensis KCTC 2396 simultaneously produced red antibiotic prodigiosin and undecylprodiginine. A complete set of the prodigiosin biosynthetic gene cluster has been cloned, sequenced, and successfully expressed in a heterologous host. Sequence analysis of the gene cluster revealed 14 ORFs showing high similarity to pig and red genes from Serratia spp. and Streptomyces coelicolor A3(2), respectively, and the gene organization was almost: similar to that of pig genes. These genes were named hap for Hahella prodigiosin, and determined to be transcribed as a single operon, by RT-PCR experiment. Based on the hap gene mutagenesis experiments and comparative analysis with pig and red genes, we propose a prodigiosin-biosynthetic pathway in KCTC 2396.

Keywords

References

  1. Cerdeno, A. M., M. J. Bibb, and G. L. Challis. 2001. Analysis of the prodiginine biosynthesis gene cluster of Streptomyces coelicolor A3(2): New mechanisms for chain initiation and termination in modular multienzymes. Chem. Biol. 8: 817-829 https://doi.org/10.1016/S1074-5521(01)00054-0
  2. Delcher, A. L., D. Harmon, S. Kasif, O. White, and S. L. Salzberg. 1999. Improved microbial gene identification with GLIMMER. Nucleic Acids Res. 27: 4636-4641 https://doi.org/10.1093/nar/27.23.4636
  3. Furstner, A. 2003. Chemistry and biology of roseophilin and the prodigiosin alkaloids: A survey of the last 2500 years. Angew Chem. Int. Ed. Engl. 42: 3582-3603 https://doi.org/10.1002/anie.200300582
  4. Fürstner, A., J. Grabowski, and C. W. Lehmann. 1999. Total synthesis and structural refinement of the cyclic tripyrrole pigment nonylprodigiosin. J. Org. Chem. 64: 8275-8280 https://doi.org/10.1021/jo991021i
  5. Han, S. B., H. M. Kim, Y. H. Kim, C. W. Lee, E. S. Jang, K. H. Son, S. U. Kim, and Y. K. Kim. 1998. T-cell specific immunosuppression by prodigiosin isolated from Serratia marcescens. Int. J. Immunopharmacol. 20: 1-13 https://doi.org/10.1016/S0192-0561(97)00062-3
  6. Harris, A. K., N. R. Williamson, H. Slater, A. Cox, S. Abbasi, I. Foulds, H. T. Simonsen, F. J. Leeper, and J. P. Salmond. 2004. The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation. Microbiology 50: 3547-3560
  7. Hong, M. H., Y. H. Choe, Y. J. Cho, B. Y. Ahn, and N. G. Lee. 2005. Sequencing and comparative analysis of napA genes from Helicobacter pylon strains associated with irondeficiency anemia. J. Microbiol. Biotechnol. 15: 866-872
  8. Jeong, H., J. H. Yim, C. Lee, S. H. Choi, Y. K. Park, S. H. Yoon, C. G. Hur, H. Y. Kang, D. Kim, H. H. Lee, K. H. Park, S. H. Park, H. S. Park, H. K. Lee, T. K. Oh, and J. F. Kim. 2005. Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent. Nucleic Acids Res. 33: 7066-7073 https://doi.org/10.1093/nar/gki1016
  9. Jo, Y., J. Liu, Y. Jin, Y. Yang, and J. Suh. 2005. Isolation and characterization of kasugamycin biosynthetic genes from Streptomyces kasugaensis KACC 20262. J. Microbiol. Biotechnol. 15: 491-496
  10. Kim, D., Y. Park, J. Lee, J. F. Kim, H. Jeong, Y. Lee, T. Oh, and C. Lee. 2005. Biosynthesis of four antibiotic prodiginines in the marine bacterium Hahella chejuensis KCTC 2396, abstr. O-067, p. 435. In: Abstracts of the 105th General Meeting of the American Society for Microbiology. American Society for Microbiology, Washington, D.C
  11. Montaner, B. and R. Perez-Tomas. 2003. The prodigiosins: A new family of anticancer drugs. Curr. Cancer Drug Targets 3: 57-65 https://doi.org/10.2174/1568009033333772
  12. Perez-Tomas, R., B. Montaner, E. Llagostera, and V. Soto- Cerrato. 2003. The prodigiosins, proapoptotic drugs with anticancer properties. Biochem. Pharmacol. 66: 1447-1452 https://doi.org/10.1016/S0006-2952(03)00496-9
  13. Shibata, K., M. Itoh, K. Aizawa, S. Nagaoka, N. Sasaki, P. Carninci, H. Konno, J. Akiyama, K. Nishi, T. Kitsunai, H. Tashiro, M. Itoh, N. Sumi, Y. Ishii, S. Nakamura, M. Hazama, T. Nishine, A. Harada, R. Yamamoto, H. Matsumoto, S. Sakaguchi, T. Ikegami, K. Kashiwagi, S. Fujiwake, K. Inoue, and Y. Togawa. 2002. RIKEN integrated sequence analysis (RISA) system -- 384-format sequencing pipeline with 384 multicapillary sequencer. Genome Res. 10: 1757- 1771 https://doi.org/10.1101/gr.152600
  14. Thomas, M. G., M. D. Burkart, and C. T. Walsh. 2002. Conversion of $_L$-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis. Chem. Biol. 9: 171-184 https://doi.org/10.1016/S1074-5521(02)00100-X
  15. Tsao, S. W., B. A. Rudd, X. G. He, C. J. Chang, and H. G. Floss. 1985. Identification of a red pigment from Streptomyces coelicolor A3(2) as a mixture of prodigiosin derivatives. J. Antibiot. 38: 128-131 https://doi.org/10.7164/antibiotics.38.128
  16. Williamson, N. R., H. T. Simonsen, R. A. Ahmed, G. Goldet, H. Slater, L. Woodley, F. J. Leeper, and G. P. Salmond. 2005. Biosynthesis of the red antibiotic, prodigiosin, in Serratia: Identification of a novel 2-methyl-3-n-amyl-pyrrole (MAP) assembly pathway, definition of the terminal condensing enzyme, and implications for undecylprodigiosin biosynthesis in Streptomyces. Mol. Microbiol. 56: 971-989 https://doi.org/10.1111/j.1365-2958.2005.04602.x
  17. Zhao, M. W., W. Q. Liang, D. B. Zhang, S. C. Jong, J. Y. Zhong, N. Wang, M. J. Chen, and Y. J. Pan. 2004. Analysis of squalene synthase expression during the development of Ganoderma lucidum. J. Microbiol. Biotechnol. 14: 116- 120