Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Comparison of Diluents on Liquid Storage of Korean Native Goat Spermatozoa

희석액의 종류가 재래 흑염소 액상 정액의 생존율에 미치는 영향

  • Kim, H.J. (National Livestock Research Institute) ;
  • Choe, J.Y. (National Livestock Research Institute) ;
  • Choi, S.H. (National Livestock Research Institute) ;
  • Son, D.S. (National Livestock Research Institute) ;
  • Choi, S.H. (National Livestock Research Institute) ;
  • Sang, B.D. (National Livestock Research Institute) ;
  • Han, M.H. (National Livestock Research Institute) ;
  • Ryu, I.S. (National Livestock Research Institute) ;
  • Kim, I.C. (National Livestock Research Institute) ;
  • Kim, I.H. (College of Veterinary Medicine, Chungbuk National University) ;
  • Im, K.S. (Seoul National University) ;
  • Kim, S.J. (National Livestock Research Institute) ;
  • Cho, S.R. (National Livestock Research Institute)
  • Published : 2006.12.31
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Abstract

This study was to investigate the optimal short-term storage diluents for goat spermatozoa. Semen was collected with electro-ejaculation from two goats. The collected semen was diluted in BTS and centrifuged at 500 g for 5 minutes. The supernatant was discarded and diluted with BTS, Modena or Triladyl$^{(R)}$ and stored at 4 or $17^{\circ}C$ for 8 days. The motility of spermatozoa in BTS and Modena stored at $4^{\circ}C$ was rapidly decreased at day 1. The motility of spermatozoa in BTS at $17^{\circ}C$ was decreased to 30$\sim$45% at day 2. In Modena at $17^{\circ}C$, the inappropriate motility fur artificial insemination (AI) was reached at day 4. The spermatozoa stored in Triladyl$^{(R)}$ at 4 or $17^{\circ}C$ were more viable and higher motility up to day 4. In conclusion, liquid storage of goat spermatozoa in Triladyl$^{(R)}$ at 4 or $17^{\circ}C$ for 4 days showed permissible viability for AI.

본 연구는 염소 액상 정액의 활용에 적합한 희석액을 검토하여, 국내 염소 인공수정 실용화를 위한 기초 자료를 확보하기 위하여 실시되었다. 두 마리의 흑염소 수컷으로부터 전기 자극법으로 정액을 채취하였다. 채취된 정액은 BTS로 희석하여 500g에서 5분간 원심분리하여 정장을 제거하고, BTS, Modena, Triladyl$^{(R)}$로 희석하여 $4^{\circ}C$ 냉장고나 $17^{\circ}C$ 온장고에서 8일간 보관되었다. BTS와 Modena로 희석된 정자의 운동성은 하루 만에 소멸되었으며, BTS로 희석된 정액을 $17^{\circ}C$에 보관하였을 때 2일째에 30$\sim$45%로 떨어졌으며, Modena에서는 3일째까지 인공수정에 가능한 활력을 유지하였다. 한편 Triladyl$^{(R)}$로 희석하여 $4^{\circ}C$나 $17^{\circ}C$에서 보관한 경우에는 좀더 좋은 생존성을 나타내었으며, 4일까지도 인공수정이 가능한 운동성을 보여주었다.

Keywords

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