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Diversity of Endophytic Fungi Associated with Taraxacum coreanum and Their Antifungal Activity

  • Paul Narayan Chandra (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Won-Ki (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Woo, Sung-Kyoon (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Park, Myung-Soo (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Yu, Seung-Hun (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • Published : 2006.12.31

Abstract

Endophytic fungi were isolated from healthy leaf and root samples of Taraxacum coreanum. Of the 72 isolates recovered, 39 were from leaves and 33 from roots with an isolation frequency of 54% and 46%, respectively. Based on ITS sequence analysis, 72 isolates were classified into 19 genera of which 17 were under the phylum Ascomycota and 2 were under Basidiomycota. Diverse genera were found and Alternaria, Cladosporium, Fusarium and Phoma were dominant. Out of 19 genera, Apodus, Ceriporia, Dothideales, Leptodontidium, Nemania, Neoplaconema, Phaeosphaeria, Plectosphaerella and Terfezia were new to Korea. Seventy two isolates were screened for antifungal activity, of which 10 isolates (14%) were found active at least against one of the tested fungi. Isolate 050603 had the widest antifungal spectra of activity, and isolates 050592 and 050611 were active against three plant pathogenic fungi.

Keywords

References

  1. Aghighi, S., Bonjar, G H. S., Rawashdeh, R., batayneh, S. and Saadoun, I. 2004. First report of antifungal spectra of activity of Iranian actinomycetes strains against Alernaria solani, Alternaria alternata, Fusarium solani, Phytophthora megasperma, Verticillium dahliae and Saccharomyces cerevisiae. Asian J. Plant Sci. 3: 463-471 https://doi.org/10.3923/ajps.2004.463.471
  2. Annonymous. 2004. List of Plant Diseases in Korea. 4th edition. The Korean Society of Plant Pathology, Korea
  3. Bony, S., Pichon, N., Ravel, C., Durix, A., Balfourier, F. and Guillanumin, J.J. 2001. The relationship between mycotoxin synthesis and isolate morphology in fungal endophytes of Lolium perenne. New Phytologist 152: 125 https://doi.org/10.1046/j.0028-646x.2001.00231.x
  4. Carroll, G. 1998. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 69: 2-9 https://doi.org/10.2307/1943154
  5. Carter, J. P., Spink, J., Cannon, P. F., Daniels, M. J. and Osbourn, A. E. 1999. Isolation, characterization, and sensitivity of a diverse collection of Cereal-Root-Colonizing fungi. App. Environ. Microbiol. 65: 3364-3372
  6. Duran, E. L., Plopper, L. D., Ramallo, J. C., Grandi, R. A. P., Giancoli, A. C. H. and Azevedo, J. L. 2005. The foliar fungal endophytes on citrus limon in Argentina. NRC Res. 350-355
  7. Huang, Y., Wang, J., Li, G, Zheng, Z. and Su, W. 2001. Antitumor and antifungal activities in endophytic fungi isolated from pharmaceutical plants Taxus mairei, Cephalotaxus fortunei and Torreya grandis. FEMS Immounol. Med. Microbiol. 34: 163-167
  8. Koo, H. N., Hong, S. H., Kim, C. H., Yoo, Y. H. and Kim, H. M. 2004. Taraxacum officinale induces cytotoxicity through TNF-alpha and IL-alpha secretion in Hep G2 cells. Life Sci. 74: 1149-57 https://doi.org/10.1016/j.lfs.2003.07.030
  9. Lee, T. B. 1980. Illustrated Flora of Korea. Hyang Mun Sa, Seoul Korea
  10. Miguel, A., Sagei, L. and Paul, B. 2002. Measuring diversity of endophytic fungi in leaf fragments: Does size matter? Mycopathologia 156: 41-45 https://doi.org/10.1023/A:1021362217723
  11. Rebecca, J. G., Stevan, J. B. and George, N. 2004. A community of unknown, endophytic fungi in western white pine. PNAS 101: 10107-10112 https://doi.org/10.1073/pnas.0401513101
  12. Rubini, M. R., Silva-Ribeiro, R. T., Pomella, A. W. V., Maki, C. S., Araujo, W. L., Santos, D. R. and Azevedo, J. L. 2005. Diversity of endophytic fungal community of cacao (Theobroma cacao L.) and biological control of Crinipellis perniciosa, causal agent of witches broom disease. Int. J Biol. Sci. 1: 24-33
  13. Santos, R. M. G., Rodrigues-Fo, E., Rocha, W. C. and Teixeira, M. F. S. 2003. Endophytic fungi from Melia aedarach. World J. Microbiol. Biotechnol. 19: 767-770 https://doi.org/10.1023/A:1026000731189
  14. Sardi, P., Saracchi, M., Quaroni, S., Petrolini, B., Borgonovi, G. E. and Merli, S. 1992. Isolation of endophytic Streptomyces strains from surface sterilized roots. Appl. Environ. Microbiol. 58: 2691-2693
  15. Seena, S. and Sridhar, K. R. 2004. Endophytic fungal diversity of 2 sand dune wild legumes from the southwest coast of India. Can. J Microbiol. 50: 1015-1021 https://doi.org/10.1139/w04-094
  16. Stefan, G. R., Leibinger, W., Ernst, M. and Mendgen, K. 2001. Genetic diversity of fungi closely associated with common reed. New Phytologist. 149: 589-598 https://doi.org/10.1046/j.1469-8137.2001.00038.x
  17. Strobel, G. and Daisy, B. 2003. Bioprocessing for microbial endophytes and their natural products. Microbiol. Molucular Biol. Rev. 67: 491-502 https://doi.org/10.1128/MMBR.67.4.491-502.2003
  18. Taechowisan, T., Peberdy, J. F. and Lumyong, S. 2003. Isolation of endophytic actinomycetes from selected plants and their antifungal activity. World J. Microbiol. Biotechnol. 19: 381-385 https://doi.org/10.1023/A:1023901107182
  19. Tejesvi, M., Nahesh, B., Nalini, M., Prakash, H., Kini, K., Subbiah, V. and Shetty, H. 2005. Endophytic fungal asemblanges from inner bark and twig of Terminalia arjuna W. & A. (Combretaceae). World J Michobiol Biotechnol. 21: 1535-14540 https://doi.org/10.1007/s11274-005-7579-5
  20. Yang, X., Strobel, G, Stierle, A., Hess, W. M., Lee, J. and Clardy, J. 1994. A fungal endophyte-tree relationship: Phoma sp. in Taxus wallachiana. Plant Sci. 102: 1-9 https://doi.org/10.1016/0168-9452(94)90017-5

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