The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Antifungal Activity of Chitosans on Candida albicans and Trichophyton rubrum and its Induction of Apoptosis

키토산의 Candida albicans와 Trichophyton rubrum에 대한 항진균 작용과 Apoptosis 유도작용

  • 지희윤 (건양대학교 의과대학 세포생물학교실)
  • Published : 2006.12.31
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Abstract

The antifungal activity of chitosan ($M.W.\;400,000{\sim}500,000$) and chitooligosaccharide ($M.W.\;3,500{\sim}5,000$) was investigated against Candida albicans and Trichophyton rubrum. Chitosan showed antifungal activity against C. albicans and T. rubrum at 50 and 100 ng/ml, respectively while chitooligosaccharide did not suppress the growth of fungus. The mode of antifungal activity of chitosan was found to be fungicidal activity. In order to investigate the induction of apoptosis by chitosan, exposure of phosphatidylserine on the surface of the cytoplasmic membrane was observed by the FITC-annexin V reaction. The results showed that chitosan induced apoptosis on C. albicans.

키토산및 올리고키토산의 C. albicans과 T. rubrum에 대한 항 진균작용에 대하여 조사하였다. 키토산은 C. albicans과 T. rubrum에 대하여 항 진균 효과를 나타내었으나 올리고키토산은 두 균주에 대한 성장억제를 나타내지 않았다. 키토산의 항 진균 양상은 살 진균 적 활성을 나타내었다. 키토산의 apoptosis유도에 대한 조사를 위하여 phosphatidylserine의 세포막 외부로의 노출을 FITC-Annexin 반응을 이용하여 관찰하였다. 실험결과 키토산은 C. albicans의 세포에 대하여 apoptosis를 유도하는 것을 보여주었다.

Keywords

References

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