KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Separation of 6-Gingerol from Ginger [Zingiber officinale Roscoe] and Antioxidative Activity

생강으로부터 6-Gingerol의 분리 및 항산화 활성

  • Lee, Bong-Soo (MJ Health Foods Association) ;
  • Ko, Meyong-Seok (MJ Health Foods Association) ;
  • Kim, Hyun-Jong (Research Center of Industrial Technology, Chonbuk National University) ;
  • Kwak, In-Seob (Research Center of Industrial Technology, Chonbuk National University) ;
  • Kim, Dong-Ho (Department of Bioprocess Engineering, Chonbuk National University) ;
  • Chung, Bong-Woo (Research Center of Industrial Technology, Chonbuk National University)
  • 이봉수 (무주군약초조합 부설연구소) ;
  • 고명석 (무주군약초조합 부설연구소) ;
  • 김현종 (전북대학교 공업기술연구센터) ;
  • 곽인섭 (전북대학교 공업기술연구센터) ;
  • 김동호 (전북대학교 생물공정공학과) ;
  • 정봉우 (전북대학교 공업기술연구센터)
  • Published : 2006.12.30
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Abstract

Ginger (Zingiber officinale) is widely used as a dietary condiment throughout the world. Its major constituent, 6-gingerol, exhibits diverse pharmacological activities including anti-oxidant and anti-tumor. Ginger were extracted by 0% to 95% ethanol. Maximum yield of 6-gingerol was obtained with 80% ethanol as extracting solvent at $30^{\circ}C$. We obtained increased yield (7%) of extraction by pretreatment with ultrasonication. Gingerols in the crude ginger extract was isolated by Sepacore preparative liquid chromatography on silica gel. We got the 6-gingerol which weight is 0.53 mg/mL, from fraction F9. Antioxidant effect of 6-gingerol were detected by DPPH moth(10. Its radical scavenging activity was $95{\sim}99%$ which compared with ascorbic acid.

이상의 실험결과를 종합해보면 6-gingerol의 최적추출조건은 $30^{\circ}C$에서 80% 에탄올을 이용하여 추출함이 바람직하다고 여겨진다. 또한 용매만을 이용해서 추출하는 것보다 초음파를 도입하여 추출하는 것이 7% 정도 높은 결과를 얻는 것을 확인할 수 있었다. 6-gingerol의 경우 분취크로마토그래피를 이용했을 때 비교적 높은 순도의 gingerol을 얻을 수 있었으며, F9 분획에서 $0.53\;mg/m{\ell}$의 6-gingerol를 얻었다. 6-gingerol의 항산화 활성은 아스코르브산의 95% 정도로 매우 높게 나타났다. 또한 항산화 활성을 나타내는 다른 물질이 포함되어 있다는 것을 확인할 수 있었다.

Keywords

References

  1. Lee, Y. N. (1996), Flora of Korea, pp. 1107-1109, Kyohaksa, Seoul, Korea
  2. Lee, C. B. (1979), Illustrated Flora of Korea, pp. 231-231, Hyangmoon Publish Co., Seoul, Korea
  3. Leung, A. Y. (1980), Encyclopedia of Common Natural Ingredients, pp. 270-274, John Wiley & Sons, Inc., New York, USA
  4. Cooksley, V. (1996), Aromatherapy: A Life Time Guide to Healing with Essential Oils, pp. 349-350, Prentice Hall Press, Englewood Cliffs, N.J., USA
  5. Connell D. W. and M. D. Sutherland (1969), A re-examination of gingerol, shogaol, and zingerone the pungent principle of ginger (Zingiber officinale Roscoe), Aust J Chem. 22, 1033-1043 https://doi.org/10.1071/CH9691033
  6. Connell D. W. (1970), The chemistry of the essential oil and oleoresin of ginger (Zingiber officinale Roscoe), Flavour Ind. 1, 677-693
  7. Chung, Y. K. (1997), Components of essential oil and antimicrobial of Garlic and Ginger, J. Korean Soc. Appl. Biol. Chem. 40(6), 514-518
  8. Kim, M. K., M. S. Na, J. S. Hong, and S. T. Chung (1992), Components of Flavor of Ginger by the liquid $CO_2$ extraction, J. Korean Soc. Appl. Biol. Chem. 35, 55-63
  9. Surh, Y. J (1999), Molecular mechanisms of chemopreventive effects of selected dietary and medicinal phenolic substances, Mutat. Res. 428, 305-327 https://doi.org/10.1016/S1383-5742(99)00057-5
  10. Park, K. K., K. S. Chun, J. M. Lee, S. S. Lee, and Y. J. Surh (1988), Inhibitory effects of [6]-gingerol, a major pungent principle of ginger, on phorbol ester-induced inflammation, epidennal ornithine decarboxylase activity and skin tumor promotion in ICR mice, Cancer Lett. 129, 139-144 https://doi.org/10.1016/S0304-3835(98)00081-0
  11. Bode, A. M., W. Y. Ma, Y. J. Surh, and Z. Dong (2001), Inhibition of epidermal growth factor-induced cell transfonnation and activator protein I activation by [6]-gingerol, Cancer Res. 61, 850-853
  12. Azian, M. N., M. S. Hasnah, M. S. Sazalina, and M. R. Haira (2001), Ginger oleoresin from Zingiber officinale Roscoe: Effect of sample preparation yield and quality, Paper at the Congress of Science and Technology Association of Malaysia, 1-11
  13. Aeschbach, R., J. Loliger, B. C. Scott, A. Murcia, J. Butler, B. Halliwell, and O. I. Aruoma (1994), Antioxidant actions of thymol, carbacrol, 6-gingerol, zingerone and hydroxytyrosol, Food Chem. Toxicol. 32, 31-36 https://doi.org/10.1016/0278-6915(84)90033-4
  14. Chang, W. S., Y. H. Chang, F. J. Lu, and H. C. Chiang (1994), Inhibitory effects of phenolics on xanthine oxidase, Anticancer Res. 14, 501-506
  15. Melecchi, M. I. S., V. F. Peres, C. Dariva, C. A. Zini, F. C. Abad, M. M. Martinez, and E. B. Caramao (2005), Optimization of the sonication extraction method of Hibiscus tiliaceus L. flowers, Ultrasonics Sonochem. 14
  16. Tuziuti, T., K. Yasui, Y. lida, H. Taoda, and S. Koda (2004), Effect of particle addition on sonochemical reaction, Ultrasonics 42, 597 https://doi.org/10.1016/j.ultras.2004.01.082
  17. lida, Y., K. Yasui, T. Tuziuti, and M. Sivakumar (2005), Sonochemistry and its dosimetry, Microchemical. J. 80, 159 https://doi.org/10.1016/j.microc.2004.07.016
  18. Haizhou, L., L. Pordesimo, and J. Weiss (2004), High intensity ultrasound-assisted extraction of oil from soybeans, Food Research International 37, 731 https://doi.org/10.1016/j.foodres.2004.02.016
  19. Yasui, K., T. Tuziuti, and Y. Iida (2005), Dependence of the characteristics of bubbles on types of sonochemical reactors, Ultrasonics Sonochem. 12, 347
  20. Saez, V., A. Frias-Ferrer, J. Iniesta, J. Gonzalez-Garcia, A. Aldaz, and E. Riere (2005), Characterization of a 20 KHz sonoreactor. Part I: analysis of mechanical effects by classical and numerical methods, Ultrasonics Sonochem. 12, 59 https://doi.org/10.1016/j.ultsonch.2004.06.011
  21. Kim, H. K., Y. A. Kim, S. W. Hwang, and B. S. Ko (2002), Quantitative Analysis of 6-Gingerol in the Zingiberis Rhizoma by Processing Methods, Kor. J. Pharmacogn. 33(4), 291-295