Network Structure and Dextran Formation of Jeungpyeon Made with Yeast Starter

  • Hahn, Young-Sook (Department of Food and Nutrition, Sung-shin Women's University) ;
  • Lee, Hae-Eun (Department of Food and Nutrition, Sung-shin Women's University) ;
  • Park, Ju-Yeon (Department of Food and Nutrition, Sung-shin Women's University) ;
  • Woo, Kyung-Ja (Department of Food and Nutrition, In-ha University)
  • Published : 2006.02.28

Abstract

The dextransucrase activity of microorganisms which were identified as contributing to the fermentation of jeungpyeon made with yeast was measured. The dextran generated during fermentation was quantified and the viscosity changes were measured. The mechanism of network structure formation was clarified by observing the inside of the network structure over the fermentation periods ranging from 1 to 7 hr using scanning electron microscopy (SEM). The pH of jeungpyeon batter decreased significantly as the fermentation proceeded, whereas the viscosity increased. The identified lactic acid bacteria (LAB) were Leuconostoc mesenteroides subsp. mesenteroides, Pediococcus pentosaceus, Tetragenococcus halophilus, and Leuconostoc mesenteroides subsp. dextranicum. The yeast was identified as Saccharomyces cerevisiae A/Tor. Pretorien. The dextransucrase extracted from those microorganisms showed high activity. On the other hand, the amount of dextran generated from the batter increased significantly beyond 2 hr of fermentation, and the viscosity increment showed a similar trend. The SEM photos showed that the most homogeneous fine network structure was observed in the batter fermented for 2 hr. Therefore, we assumed that the dextran that was generated by microorganisms during fermentation interacted with the components of the batter to increase the stability of the network structure.

Keywords

References

  1. Cho YH, Woo KJ, Hong SY. The studies ofjeungpyeon preparation (In standardization of preparation). Korean J. Soc. Food Sci. 10: 322-328 (1994)
  2. Moon HJ, Chang HG, Mok CK. Selection of lactic starter for the improvement ofjeungpyeon manufacturing process. Korean J. Food Sci. Technol. 31: 1241-1246 (1999)
  3. Kang MS, Kang MY. Changes in physicochemical properties of jeungpyeon (fermented and steamed rice cake) batter during fermentation time. J. Korean Soc. Food Nutr. 25: 255-260 (1996)
  4. Steinkraus KH, van Veen AG, Thiebeau, DB. Studies on Idli - an Indian fermented black gram-rice food. Food Technol. 21: 916-919 (1967)
  5. Juliano BO. Production and utilization of rice, In 'Rice: Chemistry and Technology'. Am. Assoc. Cereal Chem. St. Paul, MN, USA. p.607 (1985)
  6. Kelly WJ, Asmundson RV, Harrison GL, Huang CM. Differentiation of dextran-producing Leuconostoc strains from fermented rice cake (puto) using Pulsed-field gel electrophoresis. Int. J. Food Microbiol. 26: 345-352 (1995) https://doi.org/10.1016/0168-1605(94)00146-W
  7. Mukherjee SK, Albury MN, Pederson CS, van Veen AG, Steinkraus KH. Role of Leuconostoc mesenteroides in leavening the batter of Idli, a fermented food of India. Appl. Microbiol. 13: 227-231 (1965) https://doi.org/10.1111/j.1472-765X.1991.tb00615.x
  8. Bean MM, Elliston-Hoops EA, Nishita KD. Rice flour treatment for cake-baking applications. Cereal Chem. 60: 445-449 (1984)
  9. Kulp K, Hepburn FN, Lehmann TA. Preparation of bread without gluten. Bakers Dig. 48: 34-37 (1974)
  10. Nishita KD, Roberts RL, Bean MM, Kennedy BM. Development of a yeast-leavened rice-bread formula. Cereal Chem. 53: 626-635 (1976)
  11. Nishita KD. A yeast-leavened, rice-flour bread. J. Am. Diet. Assoc. 70: 397-399 (1977)
  12. Shin KS, Woo KJ. Study on dextran and the inside structure of jeungpyeon of adding soybean. J. East Asian Soc. Dietary Life. 11: 121-130 (2001)
  13. Shin KS, Woo KJ. Changes in adding soybean on quality and surface structure of Korean rice cake (jeungpyeon). Korean J. Soc. Food Sci. 15: 249-257 (1999)
  14. European Commission. A dextran preparation produced using Leuconostoc mesenteroides, Saccharomyces cerevisiae and Lactobacillus spp. as a novel food ingredient in bakery product (2000)
  15. Kim GY, Kim JK, Kang WW, Kim JCT, Joo GJ. Shelf-life extension of rice cake by the addition of persimmon leaf tea powder. Food Sci. Biotechnol. 14: 196-199 (2005)
  16. Miyao S, Ogawa, T. Selective media for enumerating lactic acid bacteria groups from fermented pickles. Nippon Shokuhin Kogyo Gakkaishi 35: 610-617 (1988) https://doi.org/10.3136/nskkk1962.35.9_610
  17. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31: 426 (1959) https://doi.org/10.1021/ac60147a030
  18. Oh JY, Hahn YS. Dextransucrase activity of Leuconostoc sp. Strains isolated from kimchi. Kor. J. Appl. Microbiol. Biotechnol. 27: 86-89 (1999)
  19. Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of proteindye binding. Anal. Biochem. 72: 248-254 (1976) https://doi.org/10.1016/0003-2697(76)90527-3
  20. Clarke MA, Godshall MA. Determination of dextran in raw cane sugar by Roberts Copper method; collaborative study. J. Assoc. Off. Anal. Chem. 71:276-279 (1988)
  21. Kim OW, Donal FD. Determination of dextran in raw sugar process streams. Food Sci. Biotechnol. 13: 248-252 (2004)
  22. Jeanes A Dextrans Encycl. Polym. Sci. Technol. 4: 805-824 (1966)
  23. Koboli H, Reilly OJ. Immobilization and properties of Leuconostoc mesenteroides dextransucrase, Biotechnol. Bioeng. 22:1055-1069 (1980) https://doi.org/10.1002/bit.260220513