Estimation of Korean Adult's Daily Intake of Ethyl Carbamate through Korean Commercial Alcoholic Beverages Based on the Monitoring

  • Ha, Mi-Sun (Department of Applied Biology & Chemistry, Konkuk University) ;
  • Hu, Soo-Jung (National Institute of Toxicological Research) ;
  • Park, Hee-Ra (National Institute of Toxicological Research) ;
  • Lee, Hyo-Min (National Institute of Toxicological Research) ;
  • Kwon, Ki-Sung (National Institute of Toxicological Research) ;
  • Han, Eun-Mee (Department of Applied Biology & Chemistry, Konkuk University) ;
  • Kim, Kyung-Mi (Department of Applied Biology & Chemistry, Konkuk University) ;
  • Ko, Eun-Jung (Department of Applied Biology & Chemistry, Konkuk University) ;
  • Ha, Sang-Do (Department of Food Science & Technology, Chung-Ang University) ;
  • Bae, Dong-Ho (Department of Applied Biology & Chemistry, Konkuk University)
  • Published : 2006.02.28

Abstract

Levels of ethyl carbamate, by-product produced naturally during fermentation, in Korean alcoholic beverages were determined by Gas Chromatography/mass spectrometry/selected ion mode (GC/MS/SIM), and their daily intake by Korean adult group was estimated. In GC/MS/SIM analysis 0.8-10.1, 0.5-0.8, 0.4-0.9, 3.5-689.9, 8.4-30.3, 13.9-30.0, and 1.7-11.7 ppb of ethyl carbamate were detected in soju, beer, takju, fruit wine, cheongju, whiskey, and grape wine, respectively. Maximum daily exposure of ethyl carbamate through alcoholic beverage consumption was 7.41 ng/kg body weight/day for average Korean male, with one soju brand and two fruit wine brands showing high ethyl carbamate level.

Keywords

References

  1. Battaglia R, Conacher HBS, Page BD. Ethyl carbamate (urethane) in alcoholic beverages and foods (abstract). Food Addit. Contam. 4: 477-496 (1990)
  2. Canas BJ, Harvey DC, Robinson LR, Sullivan MP, Joe Jr. FL, Diachenko GW. Ethyl carbamate levels in selected foods and beverages. J. AOAC Int. 72: 873-876 (1989)
  3. Mah JH, Yoon MY, Cha GS, Byun MW, Hwang HJ. Influence of curing and heating on formation of N-nitrosamines from biogenic amines in food model system using Korean traditional fermented fish product. Food Sci. Biotechnol. 14: 168-170 (2005)
  4. Hasegawa Y, Nakamura T, Tonogai Y, Terasawa S, Ito Y, Chiyama M. Determination of ethyl carbamate in various fermented foods by selected ion. monitoring. J. Food Protect. 53: 1058-1061 (1990) https://doi.org/10.4315/0362-028X-53.12.1058
  5. Koh EM, Kwon HJ. Determination of specific carcinogen, ethyl carbamate, in kimchi. Korean J. Food Sci. Technol. 28: 421-429 (1996)
  6. Matsudo T, Aoki K, Fokuta N, Sasaki M, Uchida K. Determination of ethyl carbamate in soy sauce and its possible precursor. J. Agr. Food Chem. 41: 352-356 (1993) https://doi.org/10.1021/jf00027a003
  7. Ough CS. Ethyl carbamate in fermented beverages and food. I. Naturally occuring ethyl carbamate. J. Agr. Food Chem. 24: 323-328 (1976) https://doi.org/10.1021/jf60204a033
  8. Uthurry CA, Varela F, Colomo B, Lepe JAS, Lombardero J, Garcia del HJR- Ethyl carbamate concentrations of typical Spanish red wines. Food Chem. 88: 329-336 (2004) https://doi.org/10.1016/j.foodchem.2004.01.063
  9. Breumley WC, Canas BJ, Perfetti GA, Mossoba MM, Sphon JA, Corneliussen PE. Quantitation of ethyl carbamate in whisky, sherry, port and wine by gas chromatography/tandem mass spectrometry using a triple quadruple mass spectrometer. Anal. Chem. 60: 975978 (1988)
  10. Cairns T, Siegmund EG, Luke MA, Doose GM. Residue levels of ethyl carbamate in wines and spirits by gas chromatography and mass spectrometry/mass spectrometry. Anal. Chem, 59: 2055-2059 (1987) https://doi.org/10.1021/ac00144a010
  11. Conacher HBS, Page BD, Lau BPY, Lawrence JF, Bailey R, Calway P, Hanchay JP, Mori B. Capillary column gas chromatographic determination of ethyl carbamate in alcoholic beverages with confirmation by gas chromatography/mass spectrometry. J. AOAC Int. 70: 749-751 (1987)
  12. Walker G, Winterlin W, Fouda H, Seiber J. Gas chromatographic analysis of urethane (ethyl carbamate) in wine. J. Agr. Food Chem. 22: 944-947 (1974) https://doi.org/10.1021/jf60196a007
  13. Jagerdeo E, Dugar S, Foster GO, Schenck H. Analysis of ethyl carbamate in wines using solid-phase extraction and multidimensional gas chromatography/mass spectrometry. J. Agr. Food Chem. 50: 5797-5802 (2002) https://doi.org/10.1021/jf025559s
  14. Joe Jr. FL, Kline DA, Miletta EM, Roach JAG, Roseboro EI, Fazio T. Determination of urethane in wines by gas-liquid chromatography and its confirmation by mass spectrometry. J. AOAC Int. 60: 509-516 (1977)
  15. Lachenmeier OW, Frank W, Kuballa T. Application of tandem mass spectrometry combined with gas chromatography to the routine analysis of ethyl carbamate in stone-fruit spirits. Rapid Commun. Mass Sp. 19: 108-112 (2004) https://doi.org/10.1002/rcm.1755
  16. Park GB, Lee sa Quantitative analysis of ethyl carbamate in Korean alcoholic beverages by chromatography with mass selective detection. Anal. Sci. 15: 26-30 (2002)
  17. Ma YP, Deng FQ, Chen DZ, Sun ss: Determination of ethyl carbamate in alcoholic beverages by capillary multi-dimensional gas chromatography with thermionic specific detection. J. Chromatogr. A 695: 259-265 (1995)
  18. Beland FA, Benson RW, Mellick PW, Kobatch RM, Roberts OW, Fang JI, Doerge DR. Effect of ethanol on the tumorigenicity of urethane (ethyl carbamate) in B6C3F mice. Food Chem. Toxicol. 43: 1-19 (2004)
  19. Cha SW, Lee HJ, Cho MH, Lee MH, Koh WS, Han SS, Kim JA, Lee ES, Nam DH, Jeong TC. Role of corticosteron in ethyl carbamate-induced immunosuppression in female BALB/c mice. Toxicol. Lett. 119: 173-181 (2001) https://doi.org/10.1016/S0378-4274(00)00306-4
  20. Miller YE, Dwyer-Nield LD, Keith RL, Le M, Franklin WA, Malkinson AM. Induction of high incidence of lung tumors in C57BL/6 mice with multiple ethyl carbamate injections. Cancer Lett. 198: 139-144 (2003) https://doi.org/10.1016/S0304-3835(03)00309-4
  21. Sakano K, Oikawa S, Hiraku Y, Kawanishi S. Metabolism of carcinogenic urethane to nitric oxide is involved in oxidative DNA damage. Free Radical Bio. Med. 33: 703-714 (2002) https://doi.org/10.1016/S0891-5849(02)00969-3
  22. Tomisawa M, Suemizzu H, Ohnishi Y, Maruyama C, Urano K, Usui T, Yasuhara K, Tamaoki N, Mitsumori K. Mutation analysis of vinyl carbamate or urethane induced lung tumors in ras H2 transgenic mice. Toxicol. Lett. 142: 111-117 (2003) https://doi.org/10.1016/S0378-4274(03)00018-3
  23. Ough CS. Ethyl carbamate in fermented beverages and foods . Possible formation of ethyl carbamate from diethyl dicarbonate addition to wine. J. Agr. Food Chem. 24: 328-331 (1976) https://doi.org/10.1021/jf60204a034
  24. Arena ME, Saguir MC, Nadra M. Arginine, citrulline and ornithine metabolism by lactic acid bacteria. Int. J. Food Microbiol. 52: 155-161 (1999) https://doi.org/10.1016/S0168-1605(99)00133-6
  25. Mira de OR, Liu SQ, Patchett ML, Pilone G1. Ethyl carbamate precursor citrulline formation from arginine degradation by malolactic wine lactic acid bacteria. FEMS Microbiol. Lett. 183: 31-35 (2000) https://doi.org/10.1111/j.1574-6968.2000.tb08929.x
  26. Tonon T, Lonvaud-Funel A. Arginine metabolism by wine Lactobacilli isolated from wine. Food Microbiol. 19: 451-461 (2002) https://doi.org/10.1006/fmic.2002.0502
  27. Uthurry CA, Varela F, Colomo B, Lepe JAS, Lombardero J, Garcia del HJR. Ethyl carbamate production by selected yeast and lactic acid bacteria in red wine. Food Chem. 94: 262-270 (2004)
  28. Hasnip S, Caputi A, Crews C, Brereton P. Effects of storage time and temperature on the concentration of ethyl carbamate and its precursors in wine. Food Addit. Contam. 21: 1155-1161 (2004) https://doi.org/10.1080/02652030400019851
  29. Foulke JE. Urethane in alcoholic beverages under investigation. U.S. FDAlCFSAN Consumer Report (1993)
  30. Schlatter J, Lutz WK. The carcinogenic potential of ethyl carbamate (urethane): risk assessment at human dietary exposure levels. Food Chem. Toxicol. 28: 205-211 (1990) https://doi.org/10.1016/0278-6915(90)90008-B
  31. California Environmental Protection Agency. Proposition 65 Status Report Safe Harbor Levels: No significant risk levels for carcinogens and maximum allowable dose levels for chemicals causing reproductive toxicity. California Environmental Protection Agency, USA (2004)
  32. Zimmeli B, Schlatter J. Ethyl carbamate: analytical methodology, occurrence, formation, biological activity and risk assessment. Mutat. Res.-Rev. Mutat. 259: 325-350 (1991) https://doi.org/10.1016/0165-1218(91)90126-7
  33. Ministry of Health and Welfare. Korean health and nutrition survey and the report. Ministry of Health and Welfare, Korea (2002)
  34. Kim DH, Song HP, Choi ID, Kim BK, Cheong MY, Byun MW. Effect of the salt oncentration on the radiation sensitivity of some fermentative microorganisms inhabiting salted and fermented food. Food Sci. Biotechnol. 13: 387-389 (2004)