Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Structural Characterization of Physiologically Active Polysaccharides from Natural Products (Arabidopsis)

  • Shin, Kwang-Soon (Department of Food Science and Biotechnology, Kyonggi University) ;
  • Darvill, Alan G. (Complex Carbohydrate Research Center, The University of Georgia)
  • Published : 2006.06.30
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Abstract

To determine the functions of specific cell wall polysaccharides, polysaccharides of three mutants, mur3-1, mur3-2, and mur3-3, obtained from Arabidopsis wild type, underwent structural characterization. Upon sequential separation of pectins (RG-I and RG-II) and cross-linking glycans (xyloglucan, XG), only XG was affected by the mud mutation. Wild-type XG contained a considerable amount of fucose, whereas the fucose level in mur3 XGs was less than 20% that of wild type. Further analysis of XGs by matrix-assisted laser-induced/ionization time-of-flight (MALDI-TOF) mass spectrometry indicated that mud lines considerably or completely lost the fucosylated XG oligosaccharides such as XXFG and XLFG and the double-galactosylated oligosaccharide XLLG $^1H$-NMR spectroscopic analyses of the XG oligosaccharides from mur3-3 plant revealed the absence of fucose and a galactose level in the galactosylated side chain that was reduced by 40% compared to that of Arabidopsis wild-type plant. In contrast, 85% less fucose and a slight loss of galactose were observed in the mur3-1 and mur3-2 lines which show normal growth habit. Of the three Arabidopsis mur3 lines studied here, mur3-3 is disrupted by a T-DNA insertion in the exon of MUR3 which encodes XG-specific galactosyltransferase, and exhibits slight dwarfism. These results indicated that the T-DNA insertion at the MUR3 locus did not induce the complete loss of galactose in XG, and that galactose, rather than fucose, in the XG side chains made a major contribution to overall wall strength.

Keywords

References

  1. Bacic A, Harris PJ, Stone BA. Vol. 14. pp. 297-371. In: The Biochemistry of Plants, Stumpf PK, Conn EE (eds). Academic, New York, NY, USA (1988)
  2. Carpita NC, Gibeaut DM. Structural models of primary cell walls in flowering plants: Consistency of molecular structure with the physical properties of the walls during growing. Plant J. 3: 1-30 (1993) https://doi.org/10.1111/j.1365-313X.1993.tb00007.x
  3. Fry SC. Loosening the ties. A new enzyme, which cuts and then reforms glycosidic bonds in the cell wall, may hold the key to plant cell growth. Curr. Biol. 3: 355-357 (1993) https://doi.org/10.1016/0960-9822(93)90199-X
  4. Hayashi T. Xyloglucans in the primary cell wall. Ann. Rev. Plant Phys. 40: 139-168 (1989) https://doi.org/10.1146/annurev.pp.40.060189.001035
  5. Kiefer LL, York WS, Oarvill AG, Albersheim P. Structure of plants cell walls XXVII. Xyloglucan isolated from suspension-cultured sycamore cell walls is O-acetylated. Phytochemistry 28: 2105-2107 (1989) https://doi.org/10.1016/S0031-9422(00)97928-7
  6. Vierhuis E, York WS, Kolli VSK, Vincken J-P, Schols HA, Van Alebeek GJWM, Voragen AGJ. Structural analyses of two arabinose containing oligosaccharides derived from olive fruit xyloglucan: XXSG and XLSG. Carbohyd. Res. 332: 285-297 (2001) https://doi.org/10.1016/S0008-6215(01)00096-9
  7. Fry SC, York WS, Albersheim P, Oarvill AG, Hayashi T, Josseleau J-P, Kato Y, Lorences EP, Maclachlan GA, McNeil M, Mort AJ, Reid JSG, Seitz HU, Selvendran RR, Voragen AGJ, White AR. An unambiguous nomenclature for xyloglucans derived oligosaccharides. Plant Physiol. 89: 1-3 (1993) https://doi.org/10.1111/j.1399-3054.1993.tb01778.x
  8. Vincken J-P, Wijsman AJM, Beldman G, Niessen WMA, Voragen AGJ. Potato xyloglucan is built from XXGG-type subunits. Carbohyd. Res. 288: 219-232 (1996) https://doi.org/10.1016/S0008-6215(96)90801-0
  9. Carpita NC, Gibeaut DM. Structural models of primary cell walls in flowering plants: Consistency of molecular structure with the physical properties of the walls during growing. Plant J. 3: 1-30 (1993) https://doi.org/10.1111/j.1365-313X.1993.tb00007.x
  10. Pauly M, Albersheim P, Darvill A, York WS. Molecular domains of the cellulose/xyloglucans network in the cell walls of the higher plants. Plant J. 20: 629-639 (1999) https://doi.org/10.1046/j.1365-313X.1999.00630.x
  11. Levy S, York WS, Stuikeprill R, Meyer B, Staehelin LA. Simulations of the static and dynamic molecular conformations of xyloglucan: the role of the fucosylated side-chain in surface-specific side-chain folding. Plant J. 1: 195-215 (1991) https://doi.org/10.1111/j.1365-313X.1991.00195.x
  12. Levy S, Maclachlan G, Staehelin LA. Xyloglucan sidechains modulate binding to cellulose during in vitro binding assays as predicted by conformational dynamics simulations. Plant J. 11: 373-386 (1997) https://doi.org/10.1046/j.1365-313X.1997.11030373.x
  13. Cote F, Hahn MG. Oligosaccharins: structures and signal transduction. Plant Mol. Biol. 26: 1379-1411 (1994) https://doi.org/10.1007/BF00016481
  14. Augur C, Benhamou N, Darvill A, Albersheim P. Purification, characterization, and cell wall localization of all ${\alpha}$-fucosidase that inactivates a xyloglucan oligosaccharin. Plant J. 3: 415-426 (1993) https://doi.org/10.1046/j.1365-313X.1993.t01-20-00999.x
  15. Reiter W-D, Chapple C, Somerville CR. Mutants of Arabidopsis thaliana with altered cell wall polysaccharide composition. Plant J. 12: 335-345 (1997) https://doi.org/10.1046/j.1365-313X.1997.12020335.x
  16. Bonin CP, Potter I, Vanzin GF, Reiter WD. The MUR1 gene of Arabidopsis thaliana encodes an isoform of GDP-d-mannose-4,6-dehydratase, catalysing the first step in the de novo synthesis of GDP-l-fucose. P. Natl. Acad. Sci. USA 94: 2085-2090 (1997)
  17. Zablackis E, York WS, Pauly M, Hantus S, Reiter W-D, Chapple CCS, Albersheim P, Darvill A. Substitution of L-fucose by L-galactose in cell walls of Arabidopsis mur1. Science 272: 1808-1810 (1996) https://doi.org/10.1126/science.272.5269.1808
  18. Pauly M, Andersen LN, Kauppinen S, Kofod LV, York WS, Albersheim P, Darvill A. A xyloglucan-specific endo-${\beta}$-1, 4-glucanase from Aspergillus aculeatus: Expression cloning in yeast, purification and characterization of the recombinant enzyme. Glycobiology 9: 93-100 (1999) https://doi.org/10.1093/glycob/9.1.93
  19. Choi HD, Seog HM, Choi IW, Lee CH, Shin KS. Molecular structure of ${\beta}$-glucans isolated from non-waxy and waxy barley. Food Sci. Biotechnol. 13: 744-748 (2004)
  20. Lee CH, Oh SW, Kim IH, Kim YE, Hwang JH, Yu KW. Chemical properties and immunological activities of hot-water extract from leaves of saltwort. Food Sci. Biotechnol. 13: 167-171 (2004)
  21. Pauly M, Eberhard S, Albersheim P, Darvill A, York WS. Effects of the mur1 mutation on xyloglucans produced by suspension cultured Arabidopsis thaliana cells. Planta 214: 67-74 (2001) https://doi.org/10.1007/s004250100585
  22. Huisman MMH, Weel KGC, Schols HA, Voragen AGJ. Xyloglucan from soybean (Glycine max) meal is composed of XXXG-type building units. Carbohyd. Polym. 42: 185-191 (2000) https://doi.org/10.1016/S0144-8617(99)00154-X
  23. Vincken JP, York WS, Beldman G, Voragen AG. Two general branching patterns of xyloglucan, XXXG and XXGG. Plant Physiol. 114: 9-13 (1997) https://doi.org/10.1104/pp.114.1.9
  24. Madson M, Dunand C, Li X, Verma R, Vanzin GF, Caplan J, Shoue DA, Carpita NC, Reiter W-D. The MUR3 gene of Arabidopsis thaliana encodes a xyloglucan galactosyltransferase that is evolutionarily related to animal exostosins. Plant Cell 15: 1662-1670 (2003) https://doi.org/10.1105/tpc.009837
  25. Vanzin GF, Madson M, Carpita NC, Raikhel NV, Keegstra K, Reiter W-D. The mur2 mutant of Arabidopsis thaliana lacks fucosylated xyloglucan because of a lesion in fucosyltransferase AtFUT1. P. Natl. Acad. Sci. USA 99: 3340-3345 (2002)
  26. Pena MJ, Ryden P, Madson M, Smith AC, Carpita NC. The galactose residues of xyloglucan are essential to maintain mechanical strength of the primary cell walls in Arabidopsis during growth. Plant Physiol. 134: 443-451 (2004) https://doi.org/10.1104/pp.103.027508