Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Mevinolin Production by Monascus pilosus IFO 480 in Solid State Fermentation of Soymeal

  • Pyo, Young-Hee (Traditional Food Research Division, Korea Food Research Institute) ;
  • Lee, Young-Chul (Traditional Food Research Division, Korea Food Research Institute)
  • Published : 2006.08.30
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Abstract

Mevinolin, a fungal metabolite, is a potent inhibitor of 3-hydroxy-methyl-3-glutaryl-coenzyme A (HMG-CoA) reductase, the rate-controlling enzyme in cholesterol biosynthesis. In this investigation, the optimum factors for mevinolin production by Monascus pilosus IFO 480 in soymeal fermentation were studied. The highest yield of mevinolin, 2.82 mg mevinolin per g dry weight, without citrinin (a toxic fungal secondary metabolite) was obtained after 21 days of fermentation at $30^{\circ}C$ at 65% moisture content, particle size 0.6-0.9 mm, and initial substrate pH of 6.0. Mevinolin was present in the fermentation substrate predominantly in the hydroxycarboxylate form (open lactone, 92.1-97.3%), which is currently being used as a hypocholesterolemic agent.

Keywords

References

  1. Goldstein JL, Brown MS. Progress in understanding the LDL receptor and HMG-CoA reductase, two membrane proteins that regulate the plasma cholesterol. J. Lipid Res. 25: 1450-1461 (1984)
  2. Endo A. Monacolin K, a new hypocholesterolemic agent produced by a Monascus species. J. Antibiotechnol. 32: 852-854 (1979) https://doi.org/10.7164/antibiotics.32.852
  3. Manzoni M, Rollini M. Biosynthesis and biotechnological production of statins by filamentous fungi and application of these cholesterol-lowering drugs. Appl. Microbiol. Biot. 58: 555-564 (2002) https://doi.org/10.1007/s00253-002-0932-9
  4. Friedrich J, Zuzek M, Bencina M, Cimerman A, Strancar A, Radez I. High performance liquid chromatographic analysis of mevinolin as mevinolinic acid in fermentation broths. J. Chromatogr. A 704: 363-367 (1995) https://doi.org/10.1016/0021-9673(95)00096-6
  5. Li YG, Zhang F, Wang ZT, Hu ZB. Identification and chemical profiling of monacolins in red yeast rice using high-performance liquid chromatography with photodiode array detector and mass spectrometry. J. Pharmaceut. Biomed. 35: 1101-1112 (2004) https://doi.org/10.1016/j.jpba.2004.04.004
  6. Juzlova P, Martinkova L, Kren V. Secondary metabolites of the fungus Monascus: a review. J. Ind. Microbiol. 16: 163-170 (1996) https://doi.org/10.1007/BF01569999
  7. Chung SH, Suh HJ, Choi YM, Noh DO, Bae SH, Enzyme activities and inhibitory effect on angiotensin converting enzyme of Monascus-koji for kochujang production, Food Sci. Biotechnol. 8: 179-183 (1999)
  8. Ma J, Li Y, Ye Q, Li J, Hua Y, Ju D, Zhang D, Cooper R, Chang M. Constituents of red yeast rice, a traditional Chinese food and medicine. J. Agr. Food Chem. 48: 5220-5225 (2000) https://doi.org/10.1021/jf000338c
  9. Rhyu MR, Kim EY, Kim HY, Ahn BH, Yang CB. Characteristics of the red rice fermented with fungus Monascus. Food Sci. Biotechnol. 9: 21-26 (2000)
  10. Wang YZ, Ju XL, Zhou YG. The variability of citrinin production in Monascus type cultures. Food Microbiol. 22: 145-148 (2005) https://doi.org/10.1016/j.fm.2004.01.006
  11. Sabater-Vilar RFM, Fink-Gremmels J. Mutagenicity of commercial Monascus fermentation products and the role of citrinin contamination. Mutat. Res. 444: 7-16 (1999) https://doi.org/10.1016/S1383-5718(99)00095-9
  12. Norlha T, Lee IH. Protoplast preparation and regeneration from young hyphae of the citrinin producing fungus Monascus ruber. Food Sci. Biotechnol. 14: 543-546 (2005)
  13. Pandy A, Soccol CR, Mitchell D. New developments in solid state fermentation: I - bioprocesses and products. Process Biochem. 35: 1153-1169 (2000) https://doi.org/10.1016/S0032-9592(00)00152-7
  14. Pyo YH. Optimum conditions for production of mevinolin from the soybean fermented with Monascus species. Korean J. Food Sci. Technol. 38: 256-261 (2006)
  15. Buckland B, Gbewonyo K, Hallada T, Kaplan L, Masurekar P. Novel microbial products for medicine and agriculture. Elsevier, Amsterdam, Netherlands. pp. 161-169 (1989)
  16. Lai LST, Tsai TH, Wang TC, Cheng TY. The influence of culturing environments on lovastatin production by Aspergillus terreus in submerged cultures. Enzyme Microb. Tech. 36: 737-748 (2005) https://doi.org/10.1016/j.enzmictec.2004.12.021
  17. Chang YN, Hwang JC, Lee CC, Shih IL, Tzeng YM. Use of response surface methodology to optimize culture medium for production of lovastatin by Monascus ruber. Enzyme Microb. Tech. 30: 889-894 (2002) https://doi.org/10.1016/S0141-0229(02)00037-6