Changes in Isothiocyanate Levels in Korean Chinese Cabbage Leaves during Kimchi Storage

  • Hong, Eun-Young (Department of Food and Nutrition, DukSung Women's University) ;
  • Kim, Gun-Hee (Department of Food and Nutrition, DukSung Women's University)
  • Published : 2006.10.30

Abstract

Glucosinolates are hydrolyzed by the enzyme myrosinase and are mainly found in cruciferous vegetables such as Chinese cabbage (Brassica campestris L. ssp. pekinensis). lsothiocyanates (ITCs) are glucosinolate degradation products with reported anticarcinogenic properties. Korean Chinese cabbage in the form of 'kimchi' is a staple part of the Korean diet. In this study, we examined the effects of storage temperature and duration on glucosinolate, ITC, soluble sugar, and organic acid levels in kimchi. Changes in pH and the impact of various parts of the Korean Chinese cabbage being used during the preparation of the dish were also assessed. Extracted ITC levels, analyzed via gas chromatography (GC) and GC/mass spectrometry (GC/MS), were higher in the midrib parts than in the cabbage leaves after storage at both 4 and $20^{\circ}C$. During storage, organic acid levels increased while soluble sugars were depleted. The pH initially increased (after 1 day at $20^{\circ}C$, and 1 week at $4^{\circ}C$), but subsequently decreased over time at both temperatures. Glucosinolate and ITC levels increased in the beginning of storage but then generally fell during further storage. Our data suggest that acidity-related reduction in myrosinase activity during storage may decrease glucosinolate and ITC levels. The changes in these levels depended on the storage conditions and the Korean Chinese cabbage parts used for the kimchi preparation.

Keywords

References

  1. Gerber M, Boutron-Ruault MC, Hercberg S, Riboli E, Scalbert A, Sies MH. Food and cancer: state of the art about the protective effect of fruits and vegetables. B. Cancer 89: 293-312 (2002)
  2. La Vecchia C, Altieri A, Tafani A. Vegetables, fruits, antioxidants and cancer: a review ofltalian studies. Eur. J. Nutr. 40: 261-267 (2001) https://doi.org/10.1007/s394-001-8354-9
  3. Rosa EAS, Heaney RK, Fenwick GR, Portas CAM. Glucosinolates in crop plants. Hort. Sci. 19: 99-216 (1997)
  4. Fahey JW, Zalcmenn AT, Talalay P. The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56: 5-51 (2001) https://doi.org/10.1016/S0031-9422(00)00316-2
  5. Chung FL, Kelloff G, Steele V, Pittman B, Zang E, Jiao D, Rigotty J, Choi CI, Rivenson A. Chemopreventive efficacy of arylalkyl isothio-cyanates and N-acetylcysteine for lung tumorigenesis in Fischer rats. Cancer Res. 56: 772-778 (1996)
  6. Stoner GD, Morse MA. Isothiocyanates and plant polyphenols as inhibitors of lung and esophageal cancer. Cancer Lett. 114: 113-119 (1997) https://doi.org/10.1016/S0304-3835(97)04639-9
  7. Vekerk R, Dekker M, Onger WMF. Glucosinolates. pp. 29-53. In: Natural Toxicant in Food. Sheffield Academic Press, London, UK (1998)
  8. Fahey JW, Zhang Y, Talalay P. Broccoli sprouts: An exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. P. Natl. Acad. Sci. USA 94: 10367-10372 (1997)
  9. Barrett JE, Klopfenstein CF, Leipold HW. Protective effects of cruciferous seed meals and hulls against colon cancer in mice. Cancer Lett. 127: 83-88 (1998) https://doi.org/10.1016/S0304-3835(98)00024-X
  10. Chew FS. Biological effects of glucosinolates. pp. 155-181 In: Biological Active Natural Products for Potential Use in Agriculture. Cutler HG (ed). The American Chemical Society, Washington, DC, USA (1998)
  11. Park KY. The nutritional evaluation and antimutagenic and anticancer effect of kimchi. J. Korean Soc. Food Sci. Nutr. 24: 169-182 (1995)
  12. Cho EJ, Rhee SH, Kang KS, Park KY. In vitro anticancer effects of Chinese cabbage kimchi fractions. J. Korean Soc. Food Sci. Nutr. 28: 1326-1331 (1999)
  13. Park KY, Back KA, Rhee SH, Cheigh HS. Antimutagenic effect of kimchi. Food Sci. Biotechnol. 4: 141-145 (1995)
  14. Brzezinski W, Medelewski P. Determination of total glucosinolate content in rapeseed meal with thymol reagent. Z. Pflanzenzuchtung 93: 177-183 (1984)
  15. Mheen TI, Kwon TW. Effect of temperature and salt concentration on kimchi germination. Korean. J. Food Sci. Technol. 16: 443-450 (1984)
  16. Ku KH, Kang KO, Kim WJ. Some Quality Changes during Fermentation of kimchi. Korean. J. Food Sci. Technol. 20: 476-482 (1988)
  17. Kim HO, Rhee HS. Studies on the nonvolatile organic acids in kimchi fermented at different temperatures. Korean J. Food Sci. Technol. 7: 74-81 (1975)
  18. Kim MR. The changes of pungent principle and its related substances in Korean Radish kimchi during fermentation. PhD thesis, Seoul National University, Seoul, Korea (1988)
  19. Foo HL, Gronning LM, Goodenough L, Bones AM, Danielsen BE, Whiting DA, Rossiter JT. Purification and characterization of epithiospecifier protein from Brassica napus: enzymic intramolecular sulphur addition within alkenyl thiohydroximates derived from alkenyl glucosinolate hydrolysis, FEBS Lett. 468: 243-246 (2000) https://doi.org/10.1016/S0014-5793(00)01176-5
  20. Fenwick GR, Heaney RK, Mullin WJ. Glucosinolates and their breakdown products in food and food plants. CRC Cr. Rev. Food Sci. 18: 123-201 (1983)
  21. Kim MR, Rhee HS. The change of thiocyanate (Goitrogen) amount, indoylmethyl glucosinolate content and myrosinase activity in radish kimchi during fermentation. Korean J. Food. Cookery Sci. 5: 1-8 (1989)