Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Development of Detection Method for Cyclomaltodextrinase Family Genes using Degenerate PCR Primers

  • Oh, Su-Won (Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University) ;
  • Jang, Myoung-Uoon (Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University) ;
  • Jeong, Chang-Ku (Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University) ;
  • Yuk, Jeong-Bin (Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University) ;
  • Park, Jung-Mi (Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University) ;
  • Park, Kwan-Hwa (Center for Agricultural Biomaterials, and Department of Food Science and Biotechnology, School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Tae-Jip (Department of Food Science and Technology, School of Applied Life Science and Environment, Chungbuk National University)
  • Published : 2006.12.31
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Abstract

Cyclomaltodextrinases (CDases), maitogenic amylases, and neopullulanases share highly conserved primary structures and similar characteristics, and are thus classified into the same family. BLAST search has showed that a variety of bacterial strains harbor putative CDase family genes with several well-conserved motif amino acid sequences. In this study, four degenerate polymerase chain reaction (PCR) primer sets were designed for the detection of CDase genes, on the basis of their highly conserved amino acid blocks (WYQIFP, DGWRLD, LGSHDT, and KCMVW). The PCR detection conditions were optimized and the detection specificity of each for the primer sets was tested against the genomic DNAs isolated from 23 different Bacillus-associated species. Consequently, all tested primer sets evidenced successful amplification of specific PCR products in length, which share 55-98% amino acid sequence identity with known and putative CDases. The primers developed herein, therefore, can be applied for the easy and efficient detection and isolation of CDase family genes for the modification of functional food carbohydrates.

Keywords

References

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