Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Effects of Oenanthe javanica on Transcriptional Regulation of COX-2 by Inhibiting Translocation of p65 Subunit in LPS-Stimulated Murine Peritoneal Macrophages

  • Lee, Jeong-Min (Department of Food and Nutrition, Nambu University) ;
  • Kim, Hyun-Ji (Department of Food and Nutrition, Nambu University) ;
  • Choi, Hee-Jung (School of Education, Chonnam National University) ;
  • You, Yang-Hee (Department of Food and Nutrition, Chonnam National University) ;
  • Hwang, Kwon-Tack (Department of Food and Nutrition, Nambu University) ;
  • Lee, Myung-Yul (Department of Food and Nutrition, Chosun University) ;
  • Park, Chang-Soo (Department of Pathology, Chonnam National University Medical School) ;
  • Jun, Woo-Jin (Department of Food and Nutrition, Chonnam National University)
  • Published : 2006.12.31
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Abstract

The extracts of Oenanthe javanica were evaluated for their effects on the expression of cyclooxygenase-2 (COX-2), which is mediated by the translocation of the p65 subunit into the nucleus. Fractions of ethyl acetate and chloroform from 80% ethanol extracts of O. javanica exhibited inhibitory effects on the secretion of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) from lipopolysaccharide (LPS)-stimulated peritoneal macrophages; however, the aqueous- and hexane-fractions showed no significant effect. The ethyl acetate- and chloroform-fractions also reduced the COX-2 enzyme levels after 24-hr treatment. RT-PCR showed that the mRNA levels of COX-2 decreased following treatment with these fractions, suggesting that COX-2 expression is transcriptionally regulated by these extracts. We examined the effects of the chloroform- and ethyl acetate-fractions on the cytosolic activation of nuclear factor-${\kappa}B$ ($NF-{\kappa}B$, p65 subunit) and on the degradation of inhibitor-${\kappa}B{\alpha}$ ($I-{\kappa}B{\alpha}$) in order to determine the mechanism of COX-2 regulation. The LPS-stimulated activation of the p65 subunit was significantly blocked upon the addition of $50\;{\mu}g/mL$ of these fractions, and the cytosolic $I-{\kappa}B{\alpha}$ degradation process was simultaneously inhibited. These findings suggest that the inhibition of COX-2 expression by the ethyl acetate-and chloroform-fractions may result from the inhibition of p65 translocation by blocking the degradation of $I-{\kappa}B{\alpha}$; this may be the mechanistic basis for the anti-inflammatory effects of O. javanica.

Keywords

References

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