N-(2-Chloro-4-pyridyl)-N'-phenylurea Promotes Fruit Set of Ardisia pusilla

Lee, Chang Hee;Kwon, Oh Keun;Lee , Kwang Seek

  • Published : 20060000

Abstract

Experiments were initiated to screen an efficient plant bioregulator (PBR) for promoting fruit set of Ardisia pusilla in 2004 and to determine suitable concentration and treating time of a selected PBR in 2005. Three PBRs such as mepiquat chloride (MC), ρ-chlorophenoxyacetic acid (4-CPA), and N-(2-chloro-4-pyridyl)-N'- phenylurea (CPPU) were investigated for fruit set promotion. Plant growth and fruit set of one-year-old rooted cuttings foliar-sprayed with the three PBRs at each four concentrations on May 5, 2004 (about 50% flowering stage) were examined on November 11, 2004. Comparing fruit set of the control (14.9%), CPPU treatments showed the highest fruit set (45.0%) among the three PBRs and stable responses of fruit set regardless of its concentrations (2.5−20 ㎎ㆍL⁻¹). Thus, CPPU was re-evaluated for fruit set promotion in 2005, dividing foliar-spraying time by three flowering stages (20, 50, and 80%) from May 30 to June 13. CPPU treatment at 80% flowering level was the most effective in fruit set promotion than those at other levels regardless of its concentrations (5−20 ㎎ㆍL⁻¹). Fruits obtained from all treatments produced 100% normal seeds and seed germination percentages did not show significant difference between all PBRs and control. In conclusion, CPPU was found to be an efficient fruit set promoter of A. pusilla due to high fruit set and constant stability regardless of its concentrations. Moreover, A. pusilla plants treated with CPPU did not produce any seedless fruit related to parthenocarpy, which is generally found in several horticultural crops after this PBR application.

Keywords

References

  1. Antognozzi, E., F. Famiani, A. Palliotti, and A. Tombesi. 1993. Effects of CPPU (cytokinin) on kiwifruit productivity. Acta Hort. 329:150−152
  2. Biasi, R., D. Neri, N. Sugiyama, and G. Costa. 1993. $^{14}C$-CPPU uptake and distribution in developing kiwifruits and apples. Acta Hort. 329:101−104
  3. Curry, E.A. and D.W. Greene. 1993. CPPU influences fruit quality, fruit set, return bloom, and preharvest drop of apples. HortScience 28:115−119
  4. Fellman, C.D., P.E. Read, and M.A. Hosier. 1987. Effects of thidiazuron and CPPU on meristem formation and shoot proliferation. HortScience 22:1197−1200
  5. Flaishman, M.A., A. Shargal, and R.A. Stern. 2001. The synthetic cytokinin CPPU increases fruit size and yield of 'Spadona' and 'Costa' pear (Pyrus communis L.). J. Hort. Sci.Greene, D.W. 1989. CPPU influences 'McIntosh' apple crop load and fruit characteristics. HortScience 24:94−96
  6. Greene, D.W. 1993. A comparison of the effects of several cytokinins on apple fruit set and fruit quality. Acta Hort. 329:144−146
  7. Greene, D.W. 1995. Thidiazuron effects on fruit set, fruit quality, and return bloom of apples. HortScience 30:1238−1240
  8. Hayata, Y., X. Li, and Y. Osajima. 2002. Pollination and CPPU treatment increase endogenous IAA and decrease endogenous ABA in muskmelons during early development. J. Amer. Soc. Hort. Sci. 127:908−911
  9. Kang, C.K., D.W. Weon, J.D. Ryu, J.O. Lee, and Y.S. Park. 1995. Increase of berry set by mepiquat chloride in 'Kyoho' grape (Vitis labruscana B.). J. Kor. Soc. Hort. Sci. 36:354−360
  10. Kojima, K., K. Shiozaki, Y. Koshita, and M. Ishida. 1999. Changes of endogenous levels of ABA, IAA, and GA-like substances in fruitlet of parthenocarpic persimmon. J. Jpn. Soc. Hort. Sci. 68:242−247 https://doi.org/10.2503/jjshs.68.242
  11. Lee, A.K., J.K. Suh, M.S. Roh, and J.P. Slovin. 2003. Analysis of genetic relationships of Ardisia spp. using RAPD markers. J. Hort. Sci. Biotechnol. 78:24−28
  12. Lee, C.H. 2005a. Photosynthetic characteristics and growth of Ardisia pusilla in response to photosynthetic photon flux and temperature. J. Kor. Soc. Hort. Sci. 46:385−391
  13. Lee, C.H. 2005b. The use of 6-benzylaminopurine for lateral branching of Ardisia pusilla. J. Kor. Soc. Hort. Sci. 46:392−401
  14. ee, C.H., O.K. Keun, and Y.J. Kim. 2005. Rooting characteristics of stem tip cuttings in Ardisia pusilla as influenced by cutting stage, rooting medium, temperature, and plant growth regulator pretreatment. J. Kor. Soc. Hort. Sci. 46:217−224
  15. Lee, C.H., O.K. Kwon, S.Y. Choi, and H.D. Kim. 2004. Production of compact Ardisia pusilla DC. pot plant by using paclobutrazol. J. Kor. Soc. Hort. Sci. 45:270−276
  16. Lee, E.M., N.H. Song, I.H. Choi, and S.B. Lee. 2001. Enhancement of fruit set by using Bombus ignites smith and 4-CPA in protected cultivation of eggplant. J. Kor. Soc. Hort. Sci. 42:509−512
  17. Lewis, D.H., G.K. Burge, M.E. Hopping, and P.E. Jameson. 1996. Cytokinins and fruit development in the kiwifruit (Actinidia deliciosa). II. Effects of reduced pollination and CPPU application. Physiol. Plant. 98:187−195 https://doi.org/10.1111/j.1399-3054.1996.tb00691.x
  18. Looney, N.E. 1993. Improving fruit size, appearance, and other aspects of fruit crop 'quality' with plant bioregulating chemicals. Acta Hort. 329:120−127
  19. Miller, A.N. and C.S. Walsh. 1990. Indole-3-acetic acid concentration and ethylene evolution during early development in peach. Plant Growth Regul. 9:37−46 https://doi.org/10.1007/BF00025277
  20. Neri, D., R. Biasi, S. Tarini, N. Sugiyama, R. Giuliani, S. Sansavini, and G. Costa. 1993. Sink strength as related to CPPU mobility and application site in apples and kiwifruit spurs. Acta Hort. 329:77−80
  21. NeSmith, D.S. 2002. Response of rabbiteye blueberry (Vaccinium ashei Reade) to the growth regulators CPPU and gibberellic acid. HortScience 37:666−668
  22. Nickell, L.G. 1986. Effects of N-(2-chloro-4-pyridyl)-N'-phenylurea on grapes and other crops. Proc. Plant Growth Regul. Soc. 13:236−241
  23. Rademacher, W. 2000. Growth retardants: Effects on gibberellin biosynthesis and other metabolic pathways. Annu. Rev. Plant Physiol. Plant Mol. Biol. 51:501−531 https://doi.org/10.1146/annurev.arplant.51.1.501
  24. Reynolds, A.G., D.A. Wardle, C. Zurowski, and N.E. Looney. 1992. Phenylureas CPPU and thidiazuron affect yield components, fruit composition, and storage potential of four seedless grape selections. J. Amer. Soc. Hort. Sci. 117:85−89
  25. Sugiyama, N. and Y.T. Yamaki. 1995. Effects of CPPU on fruit set and fruit growth in Japanese persimmon. Sci. Hort. 60:337−343 https://doi.org/10.1016/0304-4238(94)00712-O
  26. Takeno, C. and H. Ise. 1992. Parthenocarpic fruit set and endogenous indole-3-acetic acid content in ovary of Cucumis sativus L. J. Jpn. Soc. Hort. Sci. 60:941−946 https://doi.org/10.2503/jjshs.60.941
  27. Tartarini, S., S. Sansavini, D. Neri, and M. Ventura. 1993. CPPU control of fruit morphogenesis in apple. Sci. Hort. 53:273−279 https://doi.org/10.1016/0304-4238(93)90047-T
  28. Weaver, R.J. 1972. Fruit set and development, p. 223−237. In: R.J. Weaver (ed.). Plant growth substances in agriculture. W.H. Freeman and Co., San Francisco, CA, USA
  29. Zabadal, T.J. and M.J. Bukovac. 2000. Effect of CPPU on fruit development in seedless and seeded grape cultivars. Hort- Science 35:496