Production of Chrysanthemum Transplants as Affected by Hydroponic Systems, Electrical Conductivity in Nutrient Solution, and Photosynthetic Photon Flux

kim, Sun-Ja;Hahn, Eun-Joo;Paek, Kee-Yoeup

  • Published : 20060000

Abstract

Single node cuttings of chrysanthemum (Dendranthema grandiflorum Kitam ‘Cheonsu’) propagated in bioreactors were transferred ex vitro and acclimatized for 30 days under hydroponic systems [sand, mixed substrate (peatmoss:perlite = 1:1, v/v), and deep flow technique (DFT)], electrical conductivity (EC) of nutrient solution (0.8, 1.6, and 3.0 dSㆍm⁻¹), and photosynthetic photon flux (PPF) (50, 100, and 250 μ㏖ㆍm⁻²ㆍs⁻¹). DFT and mixed substrate cultures resulted in greater plantlet growth compared with sand (control), and the highest growth was obtained in the DFT. Both PPF and EC level played a key role in the growth enhancement. Plantlet growth increased with increasing PPF and BC level, showing the highest growth at a PPF of 250 μ㏖ㆍm⁻²ㆍs⁻¹ and an EC of 3.0 dSㆍm⁻¹, A similar pattern was also observed in CO₂ uptake rate, which started to increase after 5 days of acclimatization and became significant with a linear increase at 250 μ㏖ㆍm⁻²ㆍs⁻¹ PPF combined with 3.0 dSㆍm⁻¹ EC until the end of acclimatization.

Keywords

References

  1. Bjorkman, O. 1973. Comparative studies on photosynthesis in higher plants, p. 1-63. In: A. Giese (ed.). Current topics in photobiology, photochemistry, and photophysiology. Vol. 8. Academic Press, New York, NY, USA
  2. Hahn, E.J. and K.Y. Paek. 2005. Multiplication of chrysanthemum shoots in bioreactors as affected by culture method and inoculation density of single node stems. Plant Cell Tiss. Org. Cult. 81:301-306 https://doi.org/10.1007/s11240-004-6655-0
  3. Hahn, E.J., J.H. Bae, and Y.B. Lee. 2000. Growth and photosynthetic characteristics of chrysanthemum plantlets as affected by the pH and EC of the nutrient solution in microponic culture. J. Kor. Soc. Hort. Sci. 41:12-15
  4. Jeon, M.H., M.B. Ali, E.J. Hahn, and K.Y. Paek. 2005. Effects of photon flux density on the morphology, photosynthesis, and growth of a CAM orchid, Doritaenopsis during postmicropropagation acclimatization. Plant Growth Regul. 45: 139-147 https://doi.org/10.1007/s10725-005-0337-8
  5. Kim, S.J. 2001. Effects of environmental conditions on growth and quality of chrysanthemum plantlets in bioreactor culture. MS Thesis, Chungbuk Natl. Univ., Cheongju, Korea
  6. Krause, G.H. and E. Weis. 1997. Chlorophyll fluorescence and photosynthesis: The basis. Annu. Rev. Plant Physiol. Plant Mol. BioI. 42:313-349
  7. Maloupa, E. 2002. Hydroponic systems, p. 143-178. In: D. Savvas and H. Passam (eds.). Hydroponic production of vegetables and ornamentals. Embryo Publ., Athens, Greece
  8. Marschner, H. 1983. Essential mineral elements, p. 5-12. In: Organic plant nutrition. Encyclopedia of plant physiology. Vol. 15. Springer, Berlin, Germany
  9. Piao, X.C., D. Charkrabarty, E.J. Hahn, and K.Y. Paek. 2004. The growth and photosynthetic characteristics of potato (Solanum tuberosum L.) plantlets as affected by hydroponic solution pH and EC, light, and $CO_2$, J. Amer. Soc. Hort. Sci. 129: 100-105
  10. Preece, J.E. and E.G Sutter. 1991. Acclimatization of micropropagated plants to the greenhouse and field, p. 71-94. In: P.C. Deberg and R.H. Zimmerman (eds.). Micropropagation: Technology and application. Kluwer Academic Publ., Dordretch, The Netherlands
  11. SAS Institute. 1989. SAS/STAT user's guide. Ver. 6. 4th edn. Vol. 2. SAS Inst., Cary, NC, USA
  12. Schwarz, M. 1995. Soilless culture management. Springer Verlag, Berlin, Germany
  13. Shim, S.W., EJ. Hahn, and K.Y. Paek. 2003. In vitro and ex vitro growth of grapevine rootstock '5BB' as influenced by number of air exchanges and the presence or absence of sucrose in culture media. Plant Cell Tiss. Org. Cult. 75:57-62 https://doi.org/10.1023/A:1024695510994
  14. Shim, S.W., E.J. Hahn, and K.Y. Paek. 2004. Production of grapevine rootstock '5BB' plants as influenced by bioreactor culture method, and pH and EC of nutrient solution. J. Kor. Soc. Hort. Sci. 45:49-53
  15. Sonneveld, I.C. and N. Straver. 1992. Nutrient solutions for vegetable and flowers grown in water or substrates. 9th edn. Glasshouse Crops Research Station, Naaldwijk, The Netherlands