Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Total Phenol Content, Electron Donating Ability, and Tyrosinase Inhibition Activity of Pear Cut Branch Extract

배나무 절지 추출물의 총 페놀함량, 전자공여능 및 Tyrosinase 활성 저해 효과

Lee, Song-Ju;Jang, Hong-Ki;Kim, Chan-Young;Park, Yong-Seo;Kim, Tae-Chun;Park, Dong-Won;Heo, Buk-Gu
이송주;장홍기;김찬영;박용서;김태춘;박동원;허북구

  • Published : 20060000
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Abstract

This study was conducted to examine the total phenol content, the electron donating ability, and the tyrosinase inhibition activity of pear cut branch extracts. Extracts were made by heated and distilled water, and 70% ethanol. Total phenol content of that extracted with heated and distilled water was 137 mgL-1, and that with ethanol was 1,900 mgL-1. Electron donating abilities of pear cut branch extracts with synthetic antioxidant at the concentration of 500 and 1,000 mgL-1 were 44.8% and 66.7%. However, those extracts by heated and distilled water, and ethanol at the concentration of 500 mgL-1 were 88.6% and 91.4%, and that at 1,000 mgL-1 were 91.7% and 97.7%. Tyrosinase inhibition activity of pear cut branch extracts treated with 1.0% (w/v) was 92.0%, compared with that epicatechin 9.0%, and kojic acid 89.4%.

배나무 전정가지를 열수(증류수)와 70% 에탄올로 각기 추출하여 동결건조 후 총 페놀함량, 전자공여능 및 tyrosinase 활성의 저해 효과를 조사하였다. 총 페놀 함량은 열수 추출물의 경우 137mgL-1였으며, 에탄올 추출물은 1,900mgL-1였다. 전자공여능은 합성 항산화제인 BHT의 경우 500mg/L에서는 44.8%, 1,000mg/L에서는 66.7%를 나타내었다. 이에 비해 열수와 에탄올 추출물은 500mgL-1에서는 각각 88.6%와 91.4%를 나타내었고, 1,000mgL-1 농도에서는 91.7%와 97.7%를 나타내었다. Tyrosinase 활성 저해 효과는 1.0% 처리 시 기존의 피부 미백제인 epicatechin가 9.0%, kojic acid가 89.4%인데 비해 에탄올 추출물은 92.0%였다. 이러한 결과를 고려할 때 배나무 및 배나무 절지 추출물은 천연 화장품 소재로의 응용이 기대된다.

Keywords

References

  1. Blois, M.S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26:1199-1200
  2. Choi, H.J., J.H. Park, H.S. Han, J.H. Son, G.M. Son, J.H. Bae, and C. Choi. 2004. Effect of polyphenol compound from Korean pear on lipid metabolism. Kor. J. Food Sci. Technol. 33:299-304
  3. Dewanto, V., X. Wu, K.K. Adom, and R.H. Liu. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidative activity. J. Agric. Food Chem. 50:3010-1015 https://doi.org/10.1021/jf0115589
  4. Dural, B. and K. Shetty. 2001. The stimulation of phenolics and antioxidant activity in pea elicited by genetical transformed anise root extract. J. Food Biochem. 25:361-377 https://doi.org/10.1111/j.1745-4514.2001.tb00746.x
  5. Hong, S.S., Y.P. Hong, B.S. Im, D.S. Jeong, and I.S. Shin. 2004. Influence of picking stage storage type on the fruit respiration change and panel test in 'Wonwang', 'Hwasan', and 'Mansoo' pear. Kor. J. Hort. Sci. Technol. 22:55-62
  6. Jang, H.G., T.C. Kim, Y.S. Park, J.Y. Cho, C.K Kim, B.G. Heo, and Y.J. Park. 2004. Uilization of pruned pear branch as a potential natural dye material. Kor. J. Hort. Sci. Technol. 22:375-379
  7. Kahn, V. and A. Andrawis. 1985. Inhibition of mushroom tyrosinase by tropolone. Phytochemistry 24:905-909 https://doi.org/10.1016/S0031-9422(00)83150-7
  8. Lerner, A.B. and T.B. Fitzpatrick. 1950. Biochemistry of melanin formation. Physiol. Rev. 30:91-96 https://doi.org/10.1152/physrev.1950.30.1.91
  9. Park, C.S. 2005. Component and quality characteristics of powdered green tea cultivated in Hwagae area. Kor. J. Food Preserv. 12:36-42
  10. Pawelek, J.M. and A.M. Korner. 1982. The biosynthesis of mammalian melanin. Amer. Sci. 70:136-141
  11. Ra, K.S., H.J. Suh, S.H. Chung, and J.Y. Son 1997. Antioxidant activity of solvent extract from onion skin. Kor. J. Food Sci. Technol. 29:595-6001
  12. Tomita, K., N. Oda, M. Ohbayashi, and H. Kamei. 1990. A new screening method for melanin biosynthesis inhibitors using streptomyces bikiniensis. J. Antibiotics 43:1601-1604 https://doi.org/10.7164/antibiotics.43.1601
  13. Zhang, Y.B., H.J. Choi, H.S. Han, J.H. Park, J.H. Son, J.H. Bae, T.S. Seung, B.J. An, H.G. Kim, and C. Choi. 2003. Chemical structure of polyphenol isolated from Korean pear. Kor. J. Food Sci. Technol. 35:959-967