Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

The Viruses in Carnations in Korea

한국산 카네이션에 발생하는 바이러스

Yu, Soon-Nam;Jang, Mu-Ung;Lee, Myeong-Hwa;Jeong, Ji-Hye
유순남;장무웅;이명화;정지혜

  • Published : 20060000
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Abstract

In the survey of virus diseases of carnations in Korea from 2004 to 2006, 3 viruses of carnation mottle virus (CarMV), carnation latent virus (CLV), carnation necrotic fleck virus (CNFV) were identified from the 575 carnation plants in 67 cultivars, based on shape and size of virus particles, host range, distribution pattern of virus particles and inclusion bodies in the infected cells, serological reaction, and coat protein gene. Viral infection rates were CarMV 66.44%, CLV 6.6%, CNFV 6.78% of the 575 samples surveyed. All CLV and CNFV were doubly infected with CarMV, respectively. The average of carnation plants free from these viruses was 33.56%. Carnations from different areas and propagators varied widely in the incidences of the tested viruses. Coat protein (CP) genes of CarMV-K, CarMV-J, CarMV-C isolated from carnations collected in Korea, Japan and China were amplified using specific oligonucleotide primer by RT-PCR. Sequence analysis of amplified CP gene of CarMV-K showed 99.6% nucleotide similarity to CarMV-J and CarMV-C.

2004년부터 2006년까지 한국에서 재배되고 있는 카네이션의 바이러스의 발생상황을 조사하였다. 카네이션 재배지역에서 채집된 67품종 575식물체로부터 바이러스를 검출하여 바이러스의 입자의 형태, 기주범위, 감염세포 내의 바이러스 입자 및 봉입체의 존재 형태, 혈청반응, coat protein(CP) 유전자 등을 근거로 하여 바이러스를 동정한 결과, carnation mottle virus(CarMV), carnation latent virus(CLV), carnation necrotic fleck virus(CNFV) 등 3종의 바이러스가 확인되었다. 3종의 바이러스 감염률은 CarMV가 66.44%, CLV가 6.6%, CNFV가 6.78%로 각각 확인되었고, CLV와 CNFV는 전부 CarMV와 복합감염되어 있었다. 지역과 농가에 따라 바이러스 감염률에 차이가 있었다. 이들 바이러스에 감염되지 않은 카네이션, 즉 바이러스 무병독률(viruses- free plants)은 평균 33.56%로 확인되었다. 한국, 일본, 중국에서 채집한 카네이션에서 각각 분리된 CarMV-K, CarMV-J, CarMV-C의 CP 유전자를 특이적인 올리고뉴클레오티드 프라이머를 이용하여 역전사-중합호소반응(RT-PCR)으로 증폭시킨 후에 염기서열을 분석하였다. 일본과 중국의 카네이션에서 분리한 CarMV-K의 CP 유전자의 염기서열을 CarMV-J와 CarMV-C과 비교한 결과 99.6%의 상동성을 나타냈다.

Keywords

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