Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
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Studies on the Changes of Biological Activity and Physicochemical Characteristics of Gamma Irradiated Dandelion Extracts

감마선 조사에 의한 민들레 추출물의 생리활성 및 물리화학적 변화

Byeon, Myeong-Woo;Son, Cheon-Bae;Oh, Man-Jin;Jo, Cheol-Hun;Son, Sang-Hyeok
변명우;손천배;오만진;조철훈;손상혁

  • Published : 20060000
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Abstract

This study was performed to investigate biological activity and physicochemical changes on gamma irradiated dandelion extracts. Hunter color L* and a* values of dandelion extracts increased but b* value decreased by 20 kGy of irradiation. Effect of 1.1-Diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging was not changed by irradiation but ferric reducing/antioxidant potential (FRAP) values, an indicator of antioxidant power, tend to increase by irradiation. Tyrosinase inhibition activity of irradiated dandelion extracts significantly increased (p<0.05) but xanthine oxidase inhibition activity of irradiated dandelion extracts decreased. In vitro genotoxicological safety of irradiated dandelion extracts was evaluated using Salmonella typhimurium (TA98 and TA100). The results indicated that 20 kGy gamma-irradiated dandelion extract did not show any mutagenicity. Gamma irradiation on dandelion extracts was useful method for improvement of color of dandelion extracts and indicated positive effects on physicochemical characteristics.

감마선 조사에 의한 민들레 추출물의 생리활성 및 물리화학적 변화를 살펴보았다. 민들레 추출물을 20 kGy로 감마선 조사하여 측정한 결과 감마선 조사 후 명도 및 적색도가 높아지고 황색도가 낮아짐으로써 더 밝은 색을 띄었다. DPPH를 이용한 라디칼 소거능 실험에서는 감마선조사가 소거활성에 영향을 나타나지 않았으며, FRAP을 이용한 항산화능 실험에서는 감마선 조사구가 높은 항산화능을 나타내는 경향을 보였다. 감마선 조사후 민들레 추출물에 대한 tyrosinase 활성 저해효과에서는 감마선 조사구가 비조사구에 비하여 유의적으로 높은 효과를 나타내었으며, xanthine oxidase 저해효과에서는 감마선 비조사구가 조사구에 비하여 높은 저해율을 나타내었다. 또한, 조사구 및 비조사구에 대한 유전독성학적 안전성을 평가한 결과 농도에 따른 복귀돌연변이 집락수의 증가는 보이지 않았다. 이상의 실험결과를 통해 민들레 추출물에 대한 감마선 조사는 민들레 추출물의 색상을 개선하는데 유용한 방법이며, 민들레의 생리활성에 긍정적인 영향을 미치는 것으로 사료된다. 그리고 감마선에 의한 생리활성의 변화 기작에 관한 연구는 지속적인 연구가 필요하다고 사료된다.

Keywords

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