Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
권호 표지

Gel Promoting Ability of Various Gums and Purification of Trichoderma reesei α-Galactosidase by the Affinity chromatography

Affinity chromatography에 의한 Trichoderma reesei 유래 α-galactosidase의 정제 및 gum류의 증점효과

Park, Gwi-Geun
박귀근

  • Published : 20060000
⟨ Previous Next ⟩

Abstract

a-Galactosidase was purified from the culture filtrate of Trichoderma reesei by N-E-aminocaproyl-a-Dgalacto-pyranosylamin-sepharose affinity column chromatography. The galactosidase exhibited maximum acticity at 5.0 and 60°C, and was stable in the pH and temperature ranges of 3.0 to 6.0 and 20 to 60°C, respectively. The galactose/mannose ratio of guar gum(l: 1.6), guar gum treated with purified a-galactosidase and locust bean gum(I:3.3) were investigated. The galactose /mannose ratio of guar gum was changed 1:3.2 by the treatment of purified enzyme for 24 hI. Gel-promoting property of enzyme-treated guar gum was increased when the galactose/mannose ratio was about 1:3.2. An the ratio was appeared when the guar gum was hydrolyzed by the purified enzyme for 24 hr. It is clear that enzymatic depletion of galactose from guar gum by purified a-galactosidase would lead to a significant increase in gelation ability. And also mixture of xanthan gum and guar gum, and xanthan gum, guar gum and enzymetreated copra meal were investigated.

Keywords

References

  1. Albersheim, P.,D. J. Nevins, P. D. English and A. Karr. 1967. A method for the analysis of sugars in plant cellwall polysaccharides by gas-liquid chromatography. Carbohydr. Res. 5: 340-345 https://doi.org/10.1016/S0008-6215(00)80510-8
  2. Balasubramaniam, K., P. M. Dey, and J. B. Pridham. 1974. Purification and Identification of an $\alpha$-Galactosidase from the Coconut. Biochem. Soc. Trans. 2: 1128-1130 https://doi.org/10.1042/bst0021128
  3. Beutler, E. and W. Kuhi. 1972. Purification and properties of an $\alpha$-D-galactoside galactohydrolase from the seeds of Trifolium repens. J. Biol. Chem. 7195-7200
  4. Ciucanu, I. and F. Kerek. 1984. Carbohydrate. Res. 131: 209-217 https://doi.org/10.1016/0008-6215(84)85242-8
  5. Dey, P. M. and J. B. Pridham. 1969. $\alpha$-Galactosidase from the yeast Candida javanica. J. Bio. Chem. 113: 49-55
  6. Dey, P. M. and J. B. Pridham. 1972. Advanced in Enzymology.Academic Press, New York. 36: 91-130
  7. Dey, P. M and K. Wallenfels. 1974. Characteric features of an $\alpha$-Galactosidase from mung beans. Eur. J. Biochem. 50: 107-112 https://doi.org/10.1111/j.1432-1033.1974.tb03877.x
  8. Ferrero, I., C. Rossi, M. P. Landini and P. P. Puglisi. 1978. Role of the mitochondrial protein systhesis in the catabolite repression of the Petite-negative yeast, K. lactis. Biochem. Biophys. Res. Commun. 80: 340 https://doi.org/10.1016/0006-291X(78)90682-4
  9. Hasimoto, H., C. Katayama, M. Goto and S. Kitahata. 1993. Purification and some properties of $\alpha$-galactosidase from candida guilliermondii H-404. Biosci. Biotech. biochem. 57: 372 https://doi.org/10.1271/bbb.57.372
  10. Kaneko, R., I. Kusakabe, T. Yasui, Y, Y. Sakai and K. Murakami. 1990. Substrate specificity of $\alpha$-galactosidase from Mortierella vinacea. Agric. Bio. Chem. 54: 237 https://doi.org/10.1271/bbb1961.54.237
  11. Kaneko, R. 1991. The study of galactomannan hydrolysate. Tsukuba Univ. Master's Thesis
  12. Kusakabe, I., R. Takahashi, K. Murakami, A. Maekawa, and T. Suzuki. Preparation of crystalline $\beta$-1,4-mannooligosaccharides from copra mannan by a mannanase from Streptomyces. 1983. Agric. Biol. Chem. 47: 2391 https://doi.org/10.1271/bbb1961.47.2391
  13. Kusakabe, I., R. Takahashi, K. Maruyama, K. Murakami, A. Maekawa, and T. Suzuki. 1985. Studies on the mannanase of Streptomyces. Japan. J. Trop. Agr. 29: 167
  14. Kusakabe, I., M. Zama, G. G. Park, K. Tubake, and K. Murakami. 1987. Preparation of $\beta$-1,4-mannobiose from Penicillium purpurogenum. Agric. Biol. Chem. 51: 2825 https://doi.org/10.1271/bbb1961.51.2825
  15. Noam, H., M F. Harold, and S. Nathan. 1973. Purification of coffee bean $\alpha$-galactosidase by affinity chromatography. BBA. 67157: 213-221
  16. Park, G. G. 1999. Development of Economic Gel Promoting Agents in the Food Industry. The Cooperative Research Center of Industry, Academia and Research Institute. 99: 184
  17. Park, G. G., I. Kusakabe, Y. Komatsu, H. Kobayashi, T. Tasui, and K. Murakami. 1987. Purification and some properties of $\beta$-mannanase from Penicillium purpurogenum. Agric.Biol. Chem. 51: 2709 https://doi.org/10.1271/bbb1961.51.2709
  18. Petek, F. and T. Dong. 1961. Purification and Properties of an $\alpha$-Galactosidase from the Coffee Bean. Enzymologia. 23: 133-142
  19. Takahashi, R., I. Kusakabe, A. Maekawa, T. Suzuki, and K. Murakami. 1983. Studies on mannanase of Actinomycetes. Japan. J. Trop. Agr. 27: 140
  20. Takahashi, R., I. Kusakabe, H. Kobayashi, K. Murakami, A. Maekawa, and T. Suzuki. 1984. Purification and some properties of $\beta$-mannanase from Streptomyces. Agric. Biol. Chem. 48: 2189 https://doi.org/10.1271/bbb1961.48.2189
  21. Zama, M., I. Kusakabe, and K. Murakami. 1985. Enzymatic preparation of crystalline mannose from copra mannan. Japan. J. Trop. Agr. 29: 221