Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Comparison of Antifungal Activites of Monoterpenes and Sesquiterpenes in Essential Oil from Chamaecyparis obtusa against Dermatophytes

피부사상균에 대한 편백정유의 Mono- 및 Sesquiterpene 항진균 활성 비교

  • Gwak, Ki-Seob (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Park, Mi-Jin (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Jeung, Eui-Bae (College of Veterinary Medicine, Chungbuk National University) ;
  • Chang, Je-Won (Enbita Co. LTD.) ;
  • Choi, In-Gyu (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
  • 곽기섭 (서울대학교 농업생명과학대학 산림과학부) ;
  • 박미진 (서울대학교 농업생명과학대학 산림과학부) ;
  • 정의배 (충북대학교 수의과대학 수의학과) ;
  • 장제원 (엔바이타(주)) ;
  • 최인규 (서울대학교 농업생명과학대학 산림과학부)
  • Received : 2006.02.24
  • Accepted : 2006.03.28
  • Published : 2006.05.25
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Abstract

This study was to compare the antifungal activitiy of monoterpenes with that of sesquiterpenes, which were major constituents of antifungal active essential oil of Chamaecyparis obtusa, to investigate which constituents had more effective against dermatophytes, and to evaluate the synergistic effect of combination of the antifungal active constituents. The antifungal activities of seven fractions (A, B, C, D, E, F, and G) from C obtusa essential oil by column chromatography were tested against Trichophyton mentagrophytes. Fraction D, E, F, and G were more active than fraction A, B, and C, and their major compounds were mono- and sesquiterpenes analyzed by GC/MS. Borneol, linalool, and ${\alpha}$-terpineol were selected as monoterpenes, and ${\alpha}$-cedrol, nerolidol, and ${\beta}$-eudesmol as sesquiterpenes, respectively. The antifungal activities of the constituents were respectively examined against Trichophyton mentagrophytes (KCTC6077), Microsporum canis (KCTC6591), and Microsporum gypseum. The constituents of sesquiterpenes were more active than those of monoterpenes. By comparing single and combined sesquiterpenes with fraction F containing the higher ratio of sesquiterpenes, combined sesquiterpenes were the most active. This result indicated that there was the synergistic antifungal effect against dermatophytes when sesquiterpenes were combined together.

본 연구는 피부사상균에 항진균 활성을 보이는 편백정유에 대하여 이를 구성하고 있는 mono- 및 sesquiterpene 성분들 중 어떠한 성분들에 의해 항진균 활성이 나타나는지 탐색하고 성분들 간의 synergy 효과가 존재하는지 알아보고자 피부사상균인 Trichophyton mentagrophytes (KCTC6077), Microsporum canis (KCTC6591), Microsporum gypseum에 대하여 디스크확산법, 한천희석법을 이용하여 항진균 활성을 평가, 비교하였다. Column chromatography를 이용하여 편백정유를 7개(A, B, C, D, E, F, G)로 분획하고, 각각의 분획들에 대하여 한천희석법을 이용하여 T. mentagrophytes에 대한 항진균 활성을 실험한 결과, 분획 D, E, F, G의 항진균 활성이 높았다. GC/MS 분석을 실시하여 높은 항진균 활성을 나타내는 분획의 주성분을 확인하고 그 중 monoterpene인 borneol, linalool, ${\alpha}$-terpineol과, sesquiterpene인 ${\alpha}$-cedrol, nerolidol, ${\beta}$-eudesmol을 선택하였다. 단일 terpene의 항진균 활성 비교 시 monoterpene보다 sesquiterpene이 더 높은 활성을 보였다. 높은 활성을 보이는 sesquiterpene의 단일 및 혼합 성분들과 편백정유의 분획 중 항진균 활성이 높고 sesquiterpene의 함유량이 높은 분획 F의 비교 시, sesquiterpene의 조합 성분들이 높은 항진균 활성을 나타냈다. 이는 sesquiterpene 성분들의 혼합에 의하여 항진균 활성의 synergy 효과가 발현됨을 의미하였다.

Keywords

Acknowledgement

Supported by : 농림기술관리센타

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