Laboraroty Animal Research

Korean Association for Laboratory Animal Science (한국실험동물학회)
권호 표지

Single Oral Dose Toxicity Test of Water Extracts of Mori radicis Cortex in ICR Mouse

Ree, Jung-Woo;Seong, Seung-Kyoo;Lee, Sang-Ho;Kim, Yong-Sung;Leem, Moon-Jeong;Oh, Seong-Jun;Ryu, Jei-Man;Ku, Sae-Kwang

  • Published : 20060000
⟨ Previous Next ⟩

Abstract

The object of this study was to obtain acute information of lyophilized water extract of Mori radicis (MR) cortex in male and female mice. In order to observe the 50% lethal dose (LD₅₀), approximate lethal dosage (LD) and target organs, test articles were once orally administered to female and male ICR mice at dose levels of 2,000 and 0 (control) ㎎/㎏ (body weight) according to the recommendation of KFDA Guidelines. The mortality and changes in body weight, clinical signs and gross observation were monitored during 14 days after dosing, in addition to organ weight and histopathology of 12 types of principle organs. As the results, we could not find any mortality, clinical signs, changes in the body weight and gross findings except for significantly increase in body weight and gains at some periods and hypertrophy of popliteal lymph nodes in treated females. In addition, there were no abnormal changes related with MR extracts-treatment related abnormal changes in the organ weight and histopathology of principle organs except for increase of the frequency of hyperplasia of lymphoid cells and follicles in spleen and popliteal lymph nodes- considered as the results of pharmacological effects as enhance the immune system, and some sporadic accidental findings. The results obtained in this study suggest that the MR extracts does not cause any toxicological signs except for pharmacological effects of enhancement of immune system. The LD₅₀ and approximate LD of MR extracts in both female and male mice were considered as over 2,000 ㎎/㎏.

Keywords

References

  1. Chen, F., Nakashima, N., Kimura, I. and Kimura, M. (1995) Hypoglycemic activity and mechanisms of extracts from mulberry leaves (Folium mori) and cortex mori radicis in streptozotocin-induced diabetic mice. Yakugaku Zasshi 115, 476-482 https://doi.org/10.1248/yakushi1947.115.6_476
  2. Choi, H.J., Kim, N.J. and Kim, D.H. (2000) Inhibitory effects of crude drugs on alpha-glucosidase. Arch. Pharm. Res. 23, 261-266 https://doi.org/10.1007/BF02976457
  3. Dourish, C.T. (1987) Effects of drugs on spontaneous motor activity. In Experimental Psychopharmacology (Greenshaw, A.J. ed.), pp. 325-334, Humana Press, Clifton
  4. Hodge, H.C. and Sterner, J.H. (1949) Tabulation of toxicity classes. Am.lnd. Hyg. Q. 10, 93
  5. Hwang, S.Y., Kwon, W., Chai, H.Y., Cho, Y.M., Lee, N.J., Ryu, J.M., Shin, J.S., Kim, T.M., Cho, J.H., Kim, E.J., Park, J.H., Kang, J.K. and Kim, Y.B. (2004) Four-week repeated-dose toxicity study on Mori radicis cortex. Korean J. Lab. Anim. Sci. 20, 283-290
  6. Irwin, S. (1968) Comprehensive observational assessment: Ia. A systemic, quantitative procedure for assessing the behavioral and physiological state of the mouse. Psychopharmacology 13, 222-257 https://doi.org/10.1007/BF00401402
  7. Kim, H.M., Han, S.B., Lee, K.H., Lee, C.W., Kim, C.Y., Lee, E.J. and Huh, H. (2000) Immunomodulating activity of a polysaccharide isolated from Mori cortex radicis. Arch. Pharm. Res. 23, 240-242 https://doi.org/10.1007/BF02976452
  8. Korea Food and Drug Administration (2005) Testing Guidelines for Safety Evaluation of Drugs (Notification No. 2005-60, issued by the Korea Food and Drug Administration on October 21, 2005)
  9. Lee, J.E., Kim, H.J., Choi, E.K, Chai, H.Y., Yun, Y.W., Kim, D.J., Nam, S.Y., Lee, B.J., Ahn, B.W., Kang, H.G. and Kim, Y.B. (2003) Four-week repeated-dose toxicity study on Pinellia extract. Korean J. Lab. Anim. Sci. 19: 127-141
  10. Lee, H.S., Lee, I.G. and Ku, SK. (2006) Single oral dose toxicity study of water extracts of Picrorrhiza rhizoma in mice. J. Toxicol. Pub. Health 22, 117-126
  11. Lee, H.S, Yang, K.J., Shin, H.D., Park, B.R., Son, C.W., lang, H.J., Park, D.C., Jung, Y.M. and Ku, S.K. (2005a) Single oral dose toxicity study of Polycan, $\beta$-glucan originated from Aureobasidium in mice. J. Toxicol. Pub. Health 21, 361-365
  12. Lee, J.H, Yang, K.J., Shin, H.D., Park, B.R., Son, C.W., Jang, H.J., Park, D.C., Lee, H.S. and Ku, S.K. (2005b) Single subcutaneous dose toxicity of Polycan ?, a $\beta$-glucan originated from Aureobasidium in mice. Lab. Anim. Res. 21, 299-305
  13. Organization for Economic Co-Operation and Development (ed.)(1987) OECD Guideline (401) for Testing of Chemicals, Acute Oral Toxicity (deleted 2002)
  14. Organization for Economic Co-Operation and Development (ed.)(2001). OECD Gideline (423) for Testing of Chemicals-Acute Oral Toxicity-Acute Toxic Class Method
  15. Plata, E.J. and Murphy, W.H. (1972) Growth and haematologic properties of the BALB/wm strain of inbred mice. Lab. Anim. Sci. 22, 712-720
  16. US Environmental Protection Agency (1998) Health Effects Test Guidelines OPPTS 870.100, Acute Toxicity Testing Background. US EPA August, Washington
  17. Yamaguchi, C., Fujita, S., Obara, T. and Ueda, T. (1983) Effects of room temperature on reproduction, body weight and organ weights, food and water intakes, and hematology in mice. Exp. Anim. 32, 1-11 https://doi.org/10.1538/expanim1978.32.1_1