Journal of Korean Society on Water Environment (한국물환경학회지)

Korean Society on Water Environment (한국물환경학회)
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Granulation and Characteristics of Sludges in the Combined SHARON/ANAMMOX Processes

SHARON/ANAMMOX 결합공정에서 슬러지의 입상화와 특성

  • Hwang, In-Su (Office of Livestock Wastewater Treatment Plant, Sanju city) ;
  • Min, Kyung-Sok (Department of Environmental Engineering, Kyungpook National University)
  • 황인수 (상주시축산폐수처리사업소) ;
  • 민경석 (경북대학교 환경공학과)
  • Received : 2005.10.20
  • Accepted : 2005.12.21
  • Published : 2006.03.30
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Abstract

The combined SHARON (Single reactor system for High ammonium Removal Over Nitrite)-ANAMMOX (Anaerobic ammonium oxidation) reactor were operated in mesophilic condition ($35^{\circ}C$). In this study, microbial granulation and characteristics of SHARON and ANAMMOX sludges were investigated using settling test, Scanning Electron Microscopy (SEM) and Fluorescence In Situ Hybridization (FISH). In SHARON reactor, Aerobic granulation with diameter of 1.5~2.5 mm was accomplished but aerobic granulation was weaker than anaerobic granular sludge. Initial seed sludge of ANAMMOX reactor was used as attached media for biofilm growth. ANAMMOX sludge was more compact and rounder rather than seed sludge. Though ANAMMOX sludge has high activity, it has lower settling ability than the seed granule. The color of ANAMMOX sludge was changed from dark to redish brown granular with diameter of 1~2 mm. In FISH of ANAMMOX sludge, high fraction of Candidatus B. stuttgartiensis which paid great role of nitrogen conversion was detected. Also, FISH results reveals that ANAMMOX bacteria inhabit at inner parts near surface, having advantages in utilization of substrates and protection from oxygen inhibition.

Keywords

References

  1. 김성민, 김성훈, 김인수, 연속회분식 반응기에서 형성된 입상슬러지의 특성 규명, 한국물환경학회/대한상하수도학회 공동춘계학술발표회 논문집, P-92, 코엑스 컨퍼런스 센터, 서울, pp. 897-882 (2004)
  2. 배진연, ADEPT 공정을 이용한 돈사폐수의 처리, 경북대학교 석사학위논문 (2003)
  3. 윤주환, 장희란, 한종훈, 한혜정, SBR 에서 호기성 입상슬러지의 형성, 한국물환경학회지 20(5), pp. 534-538 (2004)
  4. 이희자, 지재성, 김광수, 안광호, 입상활성슬러지와 활성슬러지의 특성 비교, 한국물환경학회/대한상하수도학회 공동춘계학술발표회 논문집, P-84, 코엑스 컨퍼런스센터, 서울, pp. 847-850 (2004)
  5. 황인수, 민경석, ANAMMOX 공정을 이용한 돈사폐수의 질소제거 메카니즘, 대한상하수도학회/한국물환경학회 공동추계학술발표회 논문집, D-4, 대덕컨벤션센터, 대전, pp. D20-23 (2004)
  6. 황인수, 민경석, 혐기성 슬러지상 반응조를 이용한 돈사폐수의 암모늄제거, 한국물환경학회/대한상하수도학회 공동춘계 학술 발표회 논문집, D-12, 충주대학교, 충주, pp 341-344 (2002a)
  7. 황인수, 안영호, 민경석, 혐기성 슬러지상 반응조를 이용한 돈사폐수의 암모늄제거, 대한토목학회논문집, 22(4B) , pp 615-621 (2002b)
  8. 황인수, 안영호, 민경석, 돈사폐수의 혐기적 질소제거, 대한 상하수도학회/한국물환경학회 공동추계학술발표회 논문집, E-7, 대구컨벤션센터, 대구, pp. E25-27 (2002c)
  9. Ahn, Y. H., Hwang, I. S. and Min, K. S., ANAMMOX and Partial denitritation in Anaerobic Nitrogen Removal from Piggery Waste, Wat. Sci. Tech., 49(5-6), pp. 145-153 (2004)
  10. APHA, WEF and ASCE, Standard Methods for the Examination of Water and Wastewater. 20th Eds., Washington DC. USA (1998)
  11. Beeftink, H. H., Anaerobic Bacterial Aggregates, Ph.D. Thesis, University of Amsterdam, The Netherands (1987)
  12. Beun, J. J., Hendriks, A., van Loosdrecht, M. C. M., Morgenroth, E., Wilderer, P. A. and Heijcn, J. J., Aerobic Granulation in a Sequencing Batch Reactor, Wat. Sci. Tech., 33(10), pp. 2283-2290 (1999)
  13. de Beer, J. J., van den Heuvel, J. C. and Ottengraf, S. P. P., Microelectrode Measurements of the Activity Distribution in Nitrifying Bacteria Aggregates, Appl. Environ. Microbiol., 59(2), pp. 573-279 (1993)
  14. Egli, K., Fanger, U., Alvarez, P. J. J., Siegrist, H., van der Meer, J. R. and Zehnder, A. J. B., Enrichment and Characterization of an Anammox Bacterium from a Rotating Biological Contactor Treating Ammonium-rich Leachate, Arch. Microbiol., 175, pp. 198-207 (2001) https://doi.org/10.1007/s002030100255
  15. Hellinga, C., Schellen, A. A. J. C., Mulder, J. W., van Loosdrecht, M. C. M. and Heijnen, J. J., The SHARON Process; An Innovative Method for Nitrogen Removal from Ammonium Rich Wastewater, Wat. Sci. Tech., 37(9), pp. 135-142 (1998)
  16. Hwang, l. S., Min, K. S., Choi, E. and Yun, Z., Nitrogen Removal from Piggery Waste Using the Combined SHARON and ANAMMOX Reactor, Proc. of 2004 World Water Congress, Nitogen removal II, 19-24 Sep., Marrakech, Morocco (2004b)
  17. Hwang, l. S., Min, K. S. and Lee, Y. 0., Nitrogen Removal from Piggery Wastewater Using ANAMMOX Reactor, Proc. of Anaerobic Digestion 2004 10th WORLD CONGRESS, 29 Aug.-2 Sep., Montreal, Canada (2004a)
  18. Imajo, U., Tokutomi, T. and Furukawa, K., Granulation of Anammox Microorganism in Up-flow Reactors, Wat. Sci. Tech., 49(5-6), pp. 155-163 (2004)
  19. Imajo, U., Ishida, H., Fujii, T., Sugino, H., Rouse, J. D. and Furukawa, K., Detection of Anammox Activity from Activated Sludge, ASIAN WATERQUAL 2001, Asia-Pacific Regional Conference, Proc. II, Poster Presentation, Sea Hawk hotel & resort, Fukuoka, Japan, Sep. 12-16 (2001)
  20. Kwok, W. K., Picioreanu, C., Ong, S. L., van Loosdrecht, M. C. M., Ng, W. J. and Heijen, J. J., Influence of Biomass Production and Detachment Forces on Biofilm Structures in a Biofilm Airlift Suspension Reactor, Biotech. & Bioeng., 58(4), pp. 400-407 (1998) https://doi.org/10.1002/(SICI)1097-0290(19980520)58:4<400::AID-BIT7>3.0.CO;2-N
  21. Lettinga, G., van Velsen, A. F. M., Hobma, S. W., De Zeeuw, W. and Klapwijk, A., Use of Upflow Sludge Blanket Reactor Concept for Biological Wastewater Treatment, especially for Anaerobic Treatment, Biotechnol. Bioeng., 22, pp. 399-734 (1980)
  22. Lin, Y. F. and Chen, K. C., Denitrification and Methanogenesis in a Co-immobilized Mixed Culture System, Wat. Res., 29(1), pp. 35-43 (1997) https://doi.org/10.1016/0043-1354(94)00144-V
  23. Logemann, S., Schantl, J., Bijvank, S., van Loosdrecht, M., Kuenen, J. G. and Jetten, M. S. M., Molecular Microbial Diversity in a Nitifying Reactor System without Sludge Retention, FEMS Microbial. Ecol., 27, pp. 239-249 (1998) https://doi.org/10.1111/j.1574-6941.1998.tb00540.x
  24. Min, K. S., Ahn, Y. H., Hwang, I. S. and Choi, E., Feasibility of Ammonium Removal from Piggery Waste by an Anaerobic Sludge Bed Reactor, Proc. Animal Residuals 2002 Conference and Workshop, May 6-8, Washington, USA, pp. 373-386 (2002)
  25. Morgenroth, E., Sherden, T., van Loosdrecht, M. C. M., Heijen, J. J. and Wilderer, P. A., Aerobic Granular Sludge in Sequencing Batch Reactor, Wat. Res., 31(12), pp. 31913194 (1997) https://doi.org/10.1016/S0043-1354(97)00216-9
  26. Muyzer, A., van de Graaf, A. A., Robertson, L. A. and Kuenen, J. G., Anaerobic Ammonium Oxidation Discovered in a Denitrifying Fluidized Reactor, FEMS Microbiol., 59, pp. 695-700 (1993)
  27. Neef A., Amann, R. I., Schlesner, H. and Schleifer, K. H., Monitoring a Widespread Bacterial Group: In Situ Detection of Planctornycetes with 16S rRNA-targetted Probes, Microbiology, 144, pp. 3257-3266 (1998) https://doi.org/10.1099/00221287-144-12-3257
  28. Schmid, M., Twachtrnann, U., Klein, M., Strous, M., Juretschko, S., Jetten, M., S., M., Metzger, J. W., Schleifer K. H. and Wagner, M., Molecular Evidence for Genus Level Diversity of Bacteria Capable of Catalyzing Anaerobic Ammonium Oxidation, Syst. Appl. Microbiol., 23, pp. 93-106 (2000) https://doi.org/10.1016/S0723-2020(00)80050-8
  29. Strous, M., Heijnen, J. J., Kuenen, J. G. and Jetten, M. S. M., The Sequencing Batch Reactor as a Powerful Tool for the Study of Slowly Growing Anaerobic Ammonium-oxidizing Microorganisms, Appl. Microbiol. Biotechnol., 50, pp. 589-596 (1998) https://doi.org/10.1007/s002530051340
  30. Tanaka, Y., Kurosawa, H. and Murakami, H., Ethanol Production from Starch by a Co-immobilized Mixed Culture System of Aspergillus awamori and Zymomonas mobilis, Biotechnol. Bioengng., 28, pp. 1761-1768 (1986) https://doi.org/10.1002/bit.260281202
  31. Tay, J. H., Liu, Q. S. and Liu, Y., The Role of Cellular Polysaccarides In the Formation and Stability of Aerobic Granules, Lett. Appl. Microbiol., 33, pp. 222-226 (2001) https://doi.org/10.1046/j.1472-765x.2001.00986.x
  32. van de Graaf, A. A., de Bruijin, P., Robertson, L. A., Jetton, M. S. M. and Kuenen, J. G., Autotrophic Growth of Anaerobic aAmonium Oxidizing Microorganism in a Fluidized Bed Reactor, Microbiology, 142, pp. 2187-2196 (1996) https://doi.org/10.1099/13500872-142-8-2187
  33. van de Hoek, J. P., Granulation of Denitrifying Sludge, University of Wageningen, The Netherlands (1988)
  34. Wang, J. and Kang, J., The Characteristics of Anaerobic Ammonium Oxidation (ANAMMOX) by Granular Sludge an EGSB Reactor, precess Biochemistry, 40, pp. 1973-1978 (2005) https://doi.org/10.1016/j.procbio.2004.08.001